Photochemical corneal cross-linking: Evaluating the potential of a hand-held biopen
The generation of organized 3D tissue constructs that combines cells and photo-crosslinkable biomaterials has been demonstrated using a variety of 3D bioprinting technologies. These technologies have inspired the application for “in situ” bioprinting, resulting on hand-held tools called “Biopens” th...
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Elsevier
2025-04-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425000705 |
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| author | Nadina Usseglio Julia López de Andrés Juan Antonio Marchal Lorenzo Moroni Daniel Nieto |
| author_facet | Nadina Usseglio Julia López de Andrés Juan Antonio Marchal Lorenzo Moroni Daniel Nieto |
| author_sort | Nadina Usseglio |
| collection | DOAJ |
| description | The generation of organized 3D tissue constructs that combines cells and photo-crosslinkable biomaterials has been demonstrated using a variety of 3D bioprinting technologies. These technologies have inspired the application for “in situ” bioprinting, resulting on hand-held tools called “Biopens” that can transfer bioprinting capabilities directly into the hands of the surgeons. Here, we have developed and validated a biopen for ophthalmological applications, specifically for corneal stromal regeneration using photochemical corneal crosslinking (CXL), as well as for cell bioprinting and, potentially, for corneal wound healing. We used the biopen to CXL, but also for fast crosslinking processes. Cytotoxicity, cell viability and immunofluorescence experiments were performed with human corneal stroma keratocytes (HCK) loaded inside the proposed bioink compositions. Photochemical cross-linking was performed to evaluate the biopen bioprinting functionality for corneal wound closure in porcine eyes. A full-thickness penetrating incision, 5 mm in length parallel to the limbus and perpendicular to the corneal surface, was made in the enucleated porcine cornea. The mechanical properties of cornea are imitated by tuning the proposed (GelMA/PEGDA/PI) bioink composition and crosslinking parameters, which envisage the potential for being translated to a clinical environment to corneal wound closure. |
| format | Article |
| id | doaj-art-5d732a3679fe4907aab9cf52c0885ae8 |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-5d732a3679fe4907aab9cf52c0885ae82025-01-29T05:01:39ZengElsevierMaterials Today Bio2590-00642025-04-0131101512Photochemical corneal cross-linking: Evaluating the potential of a hand-held biopenNadina Usseglio0Julia López de Andrés1Juan Antonio Marchal2Lorenzo Moroni3Daniel Nieto4Advanced Biofabrication Laboratory - DNIETO LAB, Center for Interdisciplinary Chemical and Biology, CICA, University of La Coruña, SpainBiopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research (CIBM), University of Granada, Granada, E-18100, SpainBiopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research (CIBM), University of Granada, Granada, E-18100, Spain; Instituto de Investigación Biosanitaria ibs.GRANADA, University Hospitals of Granada- University of Granada, 18100, Granada, SpainComplex Tissue Regeneration Department, MERLN Institute for Technology-Inspired Regenerative Medicine, Universiteitssingel 40, 6229ER, Maastricht, the NetherlandsAdvanced Biofabrication Laboratory - DNIETO LAB, Center for Interdisciplinary Chemical and Biology, CICA, University of La Coruña, Spain; Opportunius. Axencia Galega de Innovación, 15702, Santiago de Compostela, Spain; Corresponding author. Advanced Biofabrication Laboratory - DNIETO LAB, Center for Interdisciplinary Chemical and Biology, CICA, University of La Coruña, Spain.The generation of organized 3D tissue constructs that combines cells and photo-crosslinkable biomaterials has been demonstrated using a variety of 3D bioprinting technologies. These technologies have inspired the application for “in situ” bioprinting, resulting on hand-held tools called “Biopens” that can transfer bioprinting capabilities directly into the hands of the surgeons. Here, we have developed and validated a biopen for ophthalmological applications, specifically for corneal stromal regeneration using photochemical corneal crosslinking (CXL), as well as for cell bioprinting and, potentially, for corneal wound healing. We used the biopen to CXL, but also for fast crosslinking processes. Cytotoxicity, cell viability and immunofluorescence experiments were performed with human corneal stroma keratocytes (HCK) loaded inside the proposed bioink compositions. Photochemical cross-linking was performed to evaluate the biopen bioprinting functionality for corneal wound closure in porcine eyes. A full-thickness penetrating incision, 5 mm in length parallel to the limbus and perpendicular to the corneal surface, was made in the enucleated porcine cornea. The mechanical properties of cornea are imitated by tuning the proposed (GelMA/PEGDA/PI) bioink composition and crosslinking parameters, which envisage the potential for being translated to a clinical environment to corneal wound closure.http://www.sciencedirect.com/science/article/pii/S2590006425000705Hand-held biopenBioprintingPhotocrosslinkingCornea repairingCornea Photocrosslinking |
| spellingShingle | Nadina Usseglio Julia López de Andrés Juan Antonio Marchal Lorenzo Moroni Daniel Nieto Photochemical corneal cross-linking: Evaluating the potential of a hand-held biopen Materials Today Bio Hand-held biopen Bioprinting Photocrosslinking Cornea repairing Cornea Photocrosslinking |
| title | Photochemical corneal cross-linking: Evaluating the potential of a hand-held biopen |
| title_full | Photochemical corneal cross-linking: Evaluating the potential of a hand-held biopen |
| title_fullStr | Photochemical corneal cross-linking: Evaluating the potential of a hand-held biopen |
| title_full_unstemmed | Photochemical corneal cross-linking: Evaluating the potential of a hand-held biopen |
| title_short | Photochemical corneal cross-linking: Evaluating the potential of a hand-held biopen |
| title_sort | photochemical corneal cross linking evaluating the potential of a hand held biopen |
| topic | Hand-held biopen Bioprinting Photocrosslinking Cornea repairing Cornea Photocrosslinking |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425000705 |
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