Study of Zuojin Pill in Treating Chronic Atrophic Gastritis by UPLC-Q-TOF/MS Based on Serum and Urine Metabolomics Combined with Network Pharmacology
Zuojin Pill (ZJP) is widely used for the treatment of gastrointestinal diseases, while its specific mechanism has not been systematically investigated. The aim of this study was to explore the therapeutic effects and potential mechanism of ZJP in chronic atrophic gastritis (CAG) through UPLC-Q-TOF/M...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2021/6649600 |
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| author | Shihua Wu Xing Chen Honghong Liu Ruilin Wang Jianyu Li Jianxia Wen Tao Yang Ying Wei Sichen Ren Shizhang Wei Manyi Jing Haotian Li Min Wang Houlin Xia Yanling Zhao |
| author_facet | Shihua Wu Xing Chen Honghong Liu Ruilin Wang Jianyu Li Jianxia Wen Tao Yang Ying Wei Sichen Ren Shizhang Wei Manyi Jing Haotian Li Min Wang Houlin Xia Yanling Zhao |
| author_sort | Shihua Wu |
| collection | DOAJ |
| description | Zuojin Pill (ZJP) is widely used for the treatment of gastrointestinal diseases, while its specific mechanism has not been systematically investigated. The aim of this study was to explore the therapeutic effects and potential mechanism of ZJP in chronic atrophic gastritis (CAG) through UPLC-Q-TOF/MS-based metabolomics combined with network pharmacology. ZJP and omeprazole significantly reduce contents of IL-1β, IL-6, IL-10, and iNOS and improve pathological characteristics. Metabolomic results indicated that the therapeutic effects of ZJP were mainly related to ten metabolites, namely, choline, L-threonine, hydroxypyruvic acid, creatine, taurine, succinic acid, cis-aconitic acid, citric acid, succinic acid semialdehyde, and uric acid. Pathway analysis showed that the treatment of CAG by ZJP was associated with taurine and hypotaurine metabolism; glyoxylate and dicarboxylate metabolism; glycine, serine, and threonine metabolism; glycerophospholipid metabolism; citrate cycle (TCA cycle), alanine, aspartate, and glutamate metabolism; butanoate metabolism; and purine metabolism. Validation of metabolic markers and key targets of network pharmacology through RT-PCR analysis showed that ZJP significantly downregulated a series of inflammatory markers, such as MAPK1, PKIA, RB1, SCN5A, RXRA, E2F1, PTGS1, IGF2, ADRB1, ADRA1B, PTGS2, and GABRA1. This study was the first to use a combination of metabolomics and network pharmacology to clarify the therapeutic effects of ZJP on CAG and the regulation of multiple metabolic pathways. |
| format | Article |
| id | doaj-art-5d5b148e90a947f09d48204f34a55c84 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-5d5b148e90a947f09d48204f34a55c842025-02-03T01:32:25ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792021-01-01202110.1155/2021/66496006649600Study of Zuojin Pill in Treating Chronic Atrophic Gastritis by UPLC-Q-TOF/MS Based on Serum and Urine Metabolomics Combined with Network PharmacologyShihua Wu0Xing Chen1Honghong Liu2Ruilin Wang3Jianyu Li4Jianxia Wen5Tao Yang6Ying Wei7Sichen Ren8Shizhang Wei9Manyi Jing10Haotian Li11Min Wang12Houlin Xia13Yanling Zhao14College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaIntegrative Medical Center, The Fifth Medical Center of PLA General Hospital, Beijing, ChinaIntegrative Medical Center, The Fifth Medical Center of PLA General Hospital, Beijing, ChinaIntegrative Medical Center, The Fifth Medical Center of PLA General Hospital, Beijing, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Pharmacy, The Fifth Medical Center of PLA General Hospital, Beijing, ChinaDepartment of Pharmacy, The Fifth Medical Center of PLA General Hospital, Beijing, ChinaDepartment of Pharmacy, The Fifth Medical Center of PLA General Hospital, Beijing, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Pharmacy, The Fifth Medical Center of PLA General Hospital, Beijing, ChinaZuojin Pill (ZJP) is widely used for the treatment of gastrointestinal diseases, while its specific mechanism has not been systematically investigated. The aim of this study was to explore the therapeutic effects and potential mechanism of ZJP in chronic atrophic gastritis (CAG) through UPLC-Q-TOF/MS-based metabolomics combined with network pharmacology. ZJP and omeprazole significantly reduce contents of IL-1β, IL-6, IL-10, and iNOS and improve pathological characteristics. Metabolomic results indicated that the therapeutic effects of ZJP were mainly related to ten metabolites, namely, choline, L-threonine, hydroxypyruvic acid, creatine, taurine, succinic acid, cis-aconitic acid, citric acid, succinic acid semialdehyde, and uric acid. Pathway analysis showed that the treatment of CAG by ZJP was associated with taurine and hypotaurine metabolism; glyoxylate and dicarboxylate metabolism; glycine, serine, and threonine metabolism; glycerophospholipid metabolism; citrate cycle (TCA cycle), alanine, aspartate, and glutamate metabolism; butanoate metabolism; and purine metabolism. Validation of metabolic markers and key targets of network pharmacology through RT-PCR analysis showed that ZJP significantly downregulated a series of inflammatory markers, such as MAPK1, PKIA, RB1, SCN5A, RXRA, E2F1, PTGS1, IGF2, ADRB1, ADRA1B, PTGS2, and GABRA1. This study was the first to use a combination of metabolomics and network pharmacology to clarify the therapeutic effects of ZJP on CAG and the regulation of multiple metabolic pathways.http://dx.doi.org/10.1155/2021/6649600 |
| spellingShingle | Shihua Wu Xing Chen Honghong Liu Ruilin Wang Jianyu Li Jianxia Wen Tao Yang Ying Wei Sichen Ren Shizhang Wei Manyi Jing Haotian Li Min Wang Houlin Xia Yanling Zhao Study of Zuojin Pill in Treating Chronic Atrophic Gastritis by UPLC-Q-TOF/MS Based on Serum and Urine Metabolomics Combined with Network Pharmacology International Journal of Analytical Chemistry |
| title | Study of Zuojin Pill in Treating Chronic Atrophic Gastritis by UPLC-Q-TOF/MS Based on Serum and Urine Metabolomics Combined with Network Pharmacology |
| title_full | Study of Zuojin Pill in Treating Chronic Atrophic Gastritis by UPLC-Q-TOF/MS Based on Serum and Urine Metabolomics Combined with Network Pharmacology |
| title_fullStr | Study of Zuojin Pill in Treating Chronic Atrophic Gastritis by UPLC-Q-TOF/MS Based on Serum and Urine Metabolomics Combined with Network Pharmacology |
| title_full_unstemmed | Study of Zuojin Pill in Treating Chronic Atrophic Gastritis by UPLC-Q-TOF/MS Based on Serum and Urine Metabolomics Combined with Network Pharmacology |
| title_short | Study of Zuojin Pill in Treating Chronic Atrophic Gastritis by UPLC-Q-TOF/MS Based on Serum and Urine Metabolomics Combined with Network Pharmacology |
| title_sort | study of zuojin pill in treating chronic atrophic gastritis by uplc q tof ms based on serum and urine metabolomics combined with network pharmacology |
| url | http://dx.doi.org/10.1155/2021/6649600 |
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