Protective layer β-cyclodextrin within peanut (Arachis hypogaea L.) shells’ nanoparticles enhances intracellular stable fluorescence for bioimaging applications: An in vitro and in silico study
Owing to their excellent fluorescence behavior in the past decades, carbon nanoparticles have gained remarkable attention for a wide range of biomedical applications. However, the synthesis and solubility of carbon nanoparticles in an aqueous solution remains a challenging area of study. In this wor...
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| Language: | English |
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Elsevier
2025-02-01
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| Series: | Plant Nano Biology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773111125000026 |
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| author | Fasih Bintang Ilhami Sapti Puspitarini Fitriana Astrid Rahmawati Noor Rohmah Mayasari Hepy Herliniati |
| author_facet | Fasih Bintang Ilhami Sapti Puspitarini Fitriana Astrid Rahmawati Noor Rohmah Mayasari Hepy Herliniati |
| author_sort | Fasih Bintang Ilhami |
| collection | DOAJ |
| description | Owing to their excellent fluorescence behavior in the past decades, carbon nanoparticles have gained remarkable attention for a wide range of biomedical applications. However, the synthesis and solubility of carbon nanoparticles in an aqueous solution remains a challenging area of study. In this work, we synthesized carbon nanoparticles from peanut (Arachis hypogaea L.) shells with a protected layer of β-cyclodextrin (β-CD) as a highly stable fluorescent material for bioimaging applications. These nanoparticles possess highly stable photoluminescence and optical absorption properties in an aqueous solution. Additionally, in vitro kinetic stability confirmed that β-CD-coated carbon nanoparticles are stable under serum-rich environments and exhibit antihemolytic properties. Predominantly, in vitro cytotoxicity studies clearly demonstrated that β-CD-coated carbon nanoparticles possess cytotoxicity, rapid intracellular uptake, and stable fluorescence under HeLa cells. Moreover, an in-silico study confirmed that β-CD-coated carbon nanoparticles could specifically interact with the target protein of CDK-2, which plays a role in inhibiting cancer cell growth. Hence, this new study has shown that in tracking cancer cells, high-value-added peanut (Arachis hypogaea L.) shells with a β-CD protective layer are effective in improving solubility and intracellular fluorescence. |
| format | Article |
| id | doaj-art-5d27d3ec00264f298a44d3f84cce18c8 |
| institution | Kabale University |
| issn | 2773-1111 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Nano Biology |
| spelling | doaj-art-5d27d3ec00264f298a44d3f84cce18c82025-08-20T03:42:37ZengElsevierPlant Nano Biology2773-11112025-02-011110013510.1016/j.plana.2025.100135Protective layer β-cyclodextrin within peanut (Arachis hypogaea L.) shells’ nanoparticles enhances intracellular stable fluorescence for bioimaging applications: An in vitro and in silico studyFasih Bintang Ilhami0Sapti Puspitarini1 Fitriana2Astrid Rahmawati3Noor Rohmah Mayasari4Hepy Herliniati5Department of Natural Science, Faculty of Mathematics and Natural Science, Universitas Negeri Surabaya, Surabaya 60231, Indonesia; Corresponding author.Department of Natural Science, Faculty of Mathematics and Natural Science, Universitas Negeri Surabaya, Surabaya 60231, IndonesiaDepartment of Physics, Faculty of Mathematics and Natural Science, Universitas Negeri Surabaya, Surabaya 60231, IndonesiaDepartment of Applied Chemistry, Osaka Institute of Technology, Osaka 535-8585, JapanDepartment of Nutrition, Faculty of Sports and Health Sciences, Universitas Negeri Surabaya, Surabaya 60213, IndonesiaDepartment of Medicine, Institute Kesehatan Helvetia, Medan 20124, Indonesia; Laboratory of Microbiology, Public Hospital of dr. H. Abdul Moeloek, Lampung 35112, IndonesiaOwing to their excellent fluorescence behavior in the past decades, carbon nanoparticles have gained remarkable attention for a wide range of biomedical applications. However, the synthesis and solubility of carbon nanoparticles in an aqueous solution remains a challenging area of study. In this work, we synthesized carbon nanoparticles from peanut (Arachis hypogaea L.) shells with a protected layer of β-cyclodextrin (β-CD) as a highly stable fluorescent material for bioimaging applications. These nanoparticles possess highly stable photoluminescence and optical absorption properties in an aqueous solution. Additionally, in vitro kinetic stability confirmed that β-CD-coated carbon nanoparticles are stable under serum-rich environments and exhibit antihemolytic properties. Predominantly, in vitro cytotoxicity studies clearly demonstrated that β-CD-coated carbon nanoparticles possess cytotoxicity, rapid intracellular uptake, and stable fluorescence under HeLa cells. Moreover, an in-silico study confirmed that β-CD-coated carbon nanoparticles could specifically interact with the target protein of CDK-2, which plays a role in inhibiting cancer cell growth. Hence, this new study has shown that in tracking cancer cells, high-value-added peanut (Arachis hypogaea L.) shells with a β-CD protective layer are effective in improving solubility and intracellular fluorescence.http://www.sciencedirect.com/science/article/pii/S2773111125000026Peanut shellβ-cyclodextrinNanoparticlesStable fluorescenceBioimaging |
| spellingShingle | Fasih Bintang Ilhami Sapti Puspitarini Fitriana Astrid Rahmawati Noor Rohmah Mayasari Hepy Herliniati Protective layer β-cyclodextrin within peanut (Arachis hypogaea L.) shells’ nanoparticles enhances intracellular stable fluorescence for bioimaging applications: An in vitro and in silico study Plant Nano Biology Peanut shell β-cyclodextrin Nanoparticles Stable fluorescence Bioimaging |
| title | Protective layer β-cyclodextrin within peanut (Arachis hypogaea L.) shells’ nanoparticles enhances intracellular stable fluorescence for bioimaging applications: An in vitro and in silico study |
| title_full | Protective layer β-cyclodextrin within peanut (Arachis hypogaea L.) shells’ nanoparticles enhances intracellular stable fluorescence for bioimaging applications: An in vitro and in silico study |
| title_fullStr | Protective layer β-cyclodextrin within peanut (Arachis hypogaea L.) shells’ nanoparticles enhances intracellular stable fluorescence for bioimaging applications: An in vitro and in silico study |
| title_full_unstemmed | Protective layer β-cyclodextrin within peanut (Arachis hypogaea L.) shells’ nanoparticles enhances intracellular stable fluorescence for bioimaging applications: An in vitro and in silico study |
| title_short | Protective layer β-cyclodextrin within peanut (Arachis hypogaea L.) shells’ nanoparticles enhances intracellular stable fluorescence for bioimaging applications: An in vitro and in silico study |
| title_sort | protective layer β cyclodextrin within peanut arachis hypogaea l shells nanoparticles enhances intracellular stable fluorescence for bioimaging applications an in vitro and in silico study |
| topic | Peanut shell β-cyclodextrin Nanoparticles Stable fluorescence Bioimaging |
| url | http://www.sciencedirect.com/science/article/pii/S2773111125000026 |
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