Identification and Characterization of Key Chemical Constituents in Processed Gastrodia elata Using UHPLC-MS/MS and Chemometric Methods
Gastrodia elata Blume belongs to the Orchidaceae family. G. elata is often processed when used in traditional Chinese medicine (TCM). In the current study, a traditional processing method, known as “Jianchang Bang,” was applied. Steamed and dried (S&D) G. elata was processed with ginger juice fo...
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Wiley
2019-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/4396201 |
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| author | Xide Ye Yanhong Wang Jianping Zhao Mei Wang Bharathi Avula Qiaozhen Peng Hui Ouyang Zhong Lingyun Jinlian Zhang Ikhlas A. Khan |
| author_facet | Xide Ye Yanhong Wang Jianping Zhao Mei Wang Bharathi Avula Qiaozhen Peng Hui Ouyang Zhong Lingyun Jinlian Zhang Ikhlas A. Khan |
| author_sort | Xide Ye |
| collection | DOAJ |
| description | Gastrodia elata Blume belongs to the Orchidaceae family. G. elata is often processed when used in traditional Chinese medicine (TCM). In the current study, a traditional processing method, known as “Jianchang Bang,” was applied. Steamed and dried (S&D) G. elata was processed with ginger juice for up to 5 days (GEP5D). An UHPLC-MS/MS combined with a chemometric method was developed for the analysis of processed G. elata along with the raw material as well as steamed and dried G. elata. As a result, the primary marker compounds were identified with the aid of TOF-MS and MS/MS analyses. Compared with the raw material of G. elata with GEP5D, three new parishin-type compounds were identified according to their retention time, accurate mass, and fragmentation patterns. The chromatographic peak areas for marker compounds, including S-(gastrodin)-glutathione, S-(4-hydroxybenzylamine)-glutathione, and parishin-type compounds, changed significantly. This result indicated that by applying the “Jianchang Bang” method, changes in chemical composition in G. elata contents were observed. The study also demonstrated that chemometric analysis is helpful in understanding the processing mechanism and will provide scientific support for the clinical application of G. elata. |
| format | Article |
| id | doaj-art-5d1cdbc0fc6f42abb83606afaa762a9a |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-5d1cdbc0fc6f42abb83606afaa762a9a2025-02-03T01:27:15ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732019-01-01201910.1155/2019/43962014396201Identification and Characterization of Key Chemical Constituents in Processed Gastrodia elata Using UHPLC-MS/MS and Chemometric MethodsXide Ye0Yanhong Wang1Jianping Zhao2Mei Wang3Bharathi Avula4Qiaozhen Peng5Hui Ouyang6Zhong Lingyun7Jinlian Zhang8Ikhlas A. Khan9School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 344000, ChinaNational Center for Natural Products Research, School of Pharmacy, The University of Mississippi, Jackson, MS 38677, USANational Center for Natural Products Research, School of Pharmacy, The University of Mississippi, Jackson, MS 38677, USANational Center for Natural Products Research, School of Pharmacy, The University of Mississippi, Jackson, MS 38677, USANational Center for Natural Products Research, School of Pharmacy, The University of Mississippi, Jackson, MS 38677, USASchool of Computer Information Engineering, Nanchang Hangkong University, Nanchang, Jiangxi 330063, ChinaSchool of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 344000, ChinaSchool of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 344000, ChinaSchool of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 344000, ChinaNational Center for Natural Products Research, School of Pharmacy, The University of Mississippi, Jackson, MS 38677, USAGastrodia elata Blume belongs to the Orchidaceae family. G. elata is often processed when used in traditional Chinese medicine (TCM). In the current study, a traditional processing method, known as “Jianchang Bang,” was applied. Steamed and dried (S&D) G. elata was processed with ginger juice for up to 5 days (GEP5D). An UHPLC-MS/MS combined with a chemometric method was developed for the analysis of processed G. elata along with the raw material as well as steamed and dried G. elata. As a result, the primary marker compounds were identified with the aid of TOF-MS and MS/MS analyses. Compared with the raw material of G. elata with GEP5D, three new parishin-type compounds were identified according to their retention time, accurate mass, and fragmentation patterns. The chromatographic peak areas for marker compounds, including S-(gastrodin)-glutathione, S-(4-hydroxybenzylamine)-glutathione, and parishin-type compounds, changed significantly. This result indicated that by applying the “Jianchang Bang” method, changes in chemical composition in G. elata contents were observed. The study also demonstrated that chemometric analysis is helpful in understanding the processing mechanism and will provide scientific support for the clinical application of G. elata.http://dx.doi.org/10.1155/2019/4396201 |
| spellingShingle | Xide Ye Yanhong Wang Jianping Zhao Mei Wang Bharathi Avula Qiaozhen Peng Hui Ouyang Zhong Lingyun Jinlian Zhang Ikhlas A. Khan Identification and Characterization of Key Chemical Constituents in Processed Gastrodia elata Using UHPLC-MS/MS and Chemometric Methods Journal of Analytical Methods in Chemistry |
| title | Identification and Characterization of Key Chemical Constituents in Processed Gastrodia elata Using UHPLC-MS/MS and Chemometric Methods |
| title_full | Identification and Characterization of Key Chemical Constituents in Processed Gastrodia elata Using UHPLC-MS/MS and Chemometric Methods |
| title_fullStr | Identification and Characterization of Key Chemical Constituents in Processed Gastrodia elata Using UHPLC-MS/MS and Chemometric Methods |
| title_full_unstemmed | Identification and Characterization of Key Chemical Constituents in Processed Gastrodia elata Using UHPLC-MS/MS and Chemometric Methods |
| title_short | Identification and Characterization of Key Chemical Constituents in Processed Gastrodia elata Using UHPLC-MS/MS and Chemometric Methods |
| title_sort | identification and characterization of key chemical constituents in processed gastrodia elata using uhplc ms ms and chemometric methods |
| url | http://dx.doi.org/10.1155/2019/4396201 |
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