Prevalence of foodborne zoonotic pathogens in milk and milk products along the water buffalo milk value chain in Bangladesh
Buffalo milk and milk products are considered highly valued due to their superior nutritional quality and health benefits. Therefore, assessing the prevalence of zoonotic pathogens in buffalo milk is crucial for ensuring the safety of the consumers. The present study aimed to estimate the prevalenc...
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Universidad del Zulia
2023-11-01
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| Series: | Revista Científica |
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| Online Access: | https://www.produccioncientificaluz.org/index.php/cientifica/article/view/43423 |
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| author | Shuvo Singha Gerrit Koop Md. Mizanur Rahman Fabrizio Ceciliani Md. Matiar Rahman Howlader |
| author_facet | Shuvo Singha Gerrit Koop Md. Mizanur Rahman Fabrizio Ceciliani Md. Matiar Rahman Howlader |
| author_sort | Shuvo Singha |
| collection | DOAJ |
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Buffalo milk and milk products are considered highly valued due to their superior nutritional quality and health benefits. Therefore, assessing the prevalence of zoonotic pathogens in buffalo milk is crucial for ensuring the safety of the consumers. The present study aimed to estimate the prevalence of important foodborne zoonotic pathogens in milk and milk products and identify the associated factors in the buffalo milk chain nodes in Bangladesh. One hundred and forty-three samples were collected from farm bulk milk (n = 34), middleman (n = 37), milk collection center (n = 37), and milk product shops (n= 35) and were analyzed using RT-PCR to estimate the prevalence of seven important zoonotic pathogens: Staphylococ cus aureus, Escherichia (E.) coli, Shiga toxin- producing Esch erichia (E.) coli O157:H7, Campylobacter (C.) jejuni, Listeria (L.) monocytogenes, Salmonella (S.) enterica, and Yersinia (Y.) enterocolitica. Escherichia coli was the most prevalent pathogen along the milk chain nodes. The prevalence of the pathogens was high over the buffalo milk value chain. Three classical enterotoxin- encoded genes for E. coli O157:H7 were tested e.g., eae, stx1, and stx2 of which the stx2 genotype was most prevalent and was most common in milk products (74%). The prevalence of L. monocytogenes and Y. enterocolitica were more prevalent on the farms (65 - 79%) than in the later milk value chain nodes. The prevalence of S. enterica was rather low (0 - 2.9%) in all the milk chain nodes and all the samples were negative for C. jejuni. These results suggest that buffalo milk consumers in Bangladesh are at risk for L. monocytogenes and Y. enterocolitica on the farms and E. coli O157:H7 with stx2 genotype at the milk product shops. S. enterica and C. jejuni are not frequent contaminants of the buffalo milk chain in Bangladesh. Avoiding plastic containers and dirty clothes for cleaning milk containers may help reduce contamination in the buffalo milk value chain.
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| format | Article |
| id | doaj-art-5d0f50a54739486ea904c938ab2159b1 |
| institution | Kabale University |
| issn | 0798-2259 2521-9715 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Universidad del Zulia |
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| series | Revista Científica |
| spelling | doaj-art-5d0f50a54739486ea904c938ab2159b12025-01-31T15:36:56ZengUniversidad del ZuliaRevista Científica0798-22592521-97152023-11-0133SuplementoPrevalence of foodborne zoonotic pathogens in milk and milk products along the water buffalo milk value chain in BangladeshShuvo Singha0Gerrit Koop1Md. Mizanur Rahman2Fabrizio Ceciliani3Md. Matiar Rahman Howlader4Department of Veterinary Medicine and Animal Sciences, Università degli Studi di Milano, ItalyUdder Health Bangladesh, Chattogram, BangladeshDepartment of Medicine and Surgery, Chattogram Veterinary and Animal Sciences University, Chattogram, BangladeshDepartment of Veterinary Medicine and Animal Sciences, Università degli Studi di Milano, ItalyDepartment of Physiology, Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh Buffalo milk and milk products are considered highly valued due to their superior nutritional quality and health benefits. Therefore, assessing the prevalence of zoonotic pathogens in buffalo milk is crucial for ensuring the safety of the consumers. The present study aimed to estimate the prevalence of important foodborne zoonotic pathogens in milk and milk products and identify the associated factors in the buffalo milk chain nodes in Bangladesh. One hundred and forty-three samples were collected from farm bulk milk (n = 34), middleman (n = 37), milk collection center (n = 37), and milk product shops (n= 35) and were analyzed using RT-PCR to estimate the prevalence of seven important zoonotic pathogens: Staphylococ cus aureus, Escherichia (E.) coli, Shiga toxin- producing Esch erichia (E.) coli O157:H7, Campylobacter (C.) jejuni, Listeria (L.) monocytogenes, Salmonella (S.) enterica, and Yersinia (Y.) enterocolitica. Escherichia coli was the most prevalent pathogen along the milk chain nodes. The prevalence of the pathogens was high over the buffalo milk value chain. Three classical enterotoxin- encoded genes for E. coli O157:H7 were tested e.g., eae, stx1, and stx2 of which the stx2 genotype was most prevalent and was most common in milk products (74%). The prevalence of L. monocytogenes and Y. enterocolitica were more prevalent on the farms (65 - 79%) than in the later milk value chain nodes. The prevalence of S. enterica was rather low (0 - 2.9%) in all the milk chain nodes and all the samples were negative for C. jejuni. These results suggest that buffalo milk consumers in Bangladesh are at risk for L. monocytogenes and Y. enterocolitica on the farms and E. coli O157:H7 with stx2 genotype at the milk product shops. S. enterica and C. jejuni are not frequent contaminants of the buffalo milk chain in Bangladesh. Avoiding plastic containers and dirty clothes for cleaning milk containers may help reduce contamination in the buffalo milk value chain. https://www.produccioncientificaluz.org/index.php/cientifica/article/view/43423water buffaloEscherichia coli O157H7Listeria monocytogenesYersinia enterocolitica |
| spellingShingle | Shuvo Singha Gerrit Koop Md. Mizanur Rahman Fabrizio Ceciliani Md. Matiar Rahman Howlader Prevalence of foodborne zoonotic pathogens in milk and milk products along the water buffalo milk value chain in Bangladesh Revista Científica water buffalo Escherichia coli O157 H7 Listeria monocytogenes Yersinia enterocolitica |
| title | Prevalence of foodborne zoonotic pathogens in milk and milk products along the water buffalo milk value chain in Bangladesh |
| title_full | Prevalence of foodborne zoonotic pathogens in milk and milk products along the water buffalo milk value chain in Bangladesh |
| title_fullStr | Prevalence of foodborne zoonotic pathogens in milk and milk products along the water buffalo milk value chain in Bangladesh |
| title_full_unstemmed | Prevalence of foodborne zoonotic pathogens in milk and milk products along the water buffalo milk value chain in Bangladesh |
| title_short | Prevalence of foodborne zoonotic pathogens in milk and milk products along the water buffalo milk value chain in Bangladesh |
| title_sort | prevalence of foodborne zoonotic pathogens in milk and milk products along the water buffalo milk value chain in bangladesh |
| topic | water buffalo Escherichia coli O157 H7 Listeria monocytogenes Yersinia enterocolitica |
| url | https://www.produccioncientificaluz.org/index.php/cientifica/article/view/43423 |
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