Transcripts of Anthocyanidin Reductase and Leucoanthocyanidin Reductase and Measurement of Catechin and Epicatechin in Tartary Buckwheat
Anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) play an important role in the monomeric units biosynthesis of proanthocyanidins (PAs) such as catechin and epicatechin in several plants. The aim of this study was to clone ANR and LAR genes involved in PAs biosynthesis and examine...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/726567 |
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| author | Yeon Bok Kim Aye Aye Thwe YeJi Kim Xiaohua Li Jin Woong Cho Phun Bum Park Mariadhas Valan Arasu Naif Abdullah Al-Dhabi Sun-Ju Kim Tastsuro Suzuki Kwang Hyun Jho Sang Un Park |
| author_facet | Yeon Bok Kim Aye Aye Thwe YeJi Kim Xiaohua Li Jin Woong Cho Phun Bum Park Mariadhas Valan Arasu Naif Abdullah Al-Dhabi Sun-Ju Kim Tastsuro Suzuki Kwang Hyun Jho Sang Un Park |
| author_sort | Yeon Bok Kim |
| collection | DOAJ |
| description | Anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) play an important role in the monomeric units biosynthesis of proanthocyanidins (PAs) such as catechin and epicatechin in several plants. The aim of this study was to clone ANR and LAR genes involved in PAs biosynthesis and examine the expression of these two genes in different organs under different growth conditions in two tartary buckwheat cultivars, Hokkai T8 and T10. Gene expression was carried out by quantitative real-time RT-PCR, and catechin and epicatechin content was analyzed by high performance liquid chromatography. The expression pattern of ANR and LAR did not match the accumulation pattern of PAs in different organs of two cultivars. Epicatechin content was the highest in the flowers of both cultivars and it was affected by light in only Hokkai T8 sprouts. ANR and LAR levels in tartary buckwheat might be regulated by different mechanisms for catechin and epicatechin biosynthesis under light and dark conditions. |
| format | Article |
| id | doaj-art-5d056987caf3449d8cc6799906b6ba1a |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-5d056987caf3449d8cc6799906b6ba1a2025-02-03T05:44:42ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/726567726567Transcripts of Anthocyanidin Reductase and Leucoanthocyanidin Reductase and Measurement of Catechin and Epicatechin in Tartary BuckwheatYeon Bok Kim0Aye Aye Thwe1YeJi Kim2Xiaohua Li3Jin Woong Cho4Phun Bum Park5Mariadhas Valan Arasu6Naif Abdullah Al-Dhabi7Sun-Ju Kim8Tastsuro Suzuki9Kwang Hyun Jho10Sang Un Park11Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of KoreaDepartment of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of KoreaDepartment of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of KoreaDepartment of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of KoreaDepartment of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of KoreaDepartment of Bioscience and Biotechnology, University of Suwon, San 2-2 Wauri Bongdameup, Hwasung 445-743, Republic of KoreaDepartment of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Bio-Environmental Chemistry, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of KoreaHokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Sapporo 062-8555, JapanDepartment of Business Administration, Sahmyook University, Hwarangro 815, Nowon-gu, Seoul 139-742, Republic of KoreaDepartment of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of KoreaAnthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) play an important role in the monomeric units biosynthesis of proanthocyanidins (PAs) such as catechin and epicatechin in several plants. The aim of this study was to clone ANR and LAR genes involved in PAs biosynthesis and examine the expression of these two genes in different organs under different growth conditions in two tartary buckwheat cultivars, Hokkai T8 and T10. Gene expression was carried out by quantitative real-time RT-PCR, and catechin and epicatechin content was analyzed by high performance liquid chromatography. The expression pattern of ANR and LAR did not match the accumulation pattern of PAs in different organs of two cultivars. Epicatechin content was the highest in the flowers of both cultivars and it was affected by light in only Hokkai T8 sprouts. ANR and LAR levels in tartary buckwheat might be regulated by different mechanisms for catechin and epicatechin biosynthesis under light and dark conditions.http://dx.doi.org/10.1155/2014/726567 |
| spellingShingle | Yeon Bok Kim Aye Aye Thwe YeJi Kim Xiaohua Li Jin Woong Cho Phun Bum Park Mariadhas Valan Arasu Naif Abdullah Al-Dhabi Sun-Ju Kim Tastsuro Suzuki Kwang Hyun Jho Sang Un Park Transcripts of Anthocyanidin Reductase and Leucoanthocyanidin Reductase and Measurement of Catechin and Epicatechin in Tartary Buckwheat The Scientific World Journal |
| title | Transcripts of Anthocyanidin Reductase and Leucoanthocyanidin Reductase and Measurement of Catechin and Epicatechin in Tartary Buckwheat |
| title_full | Transcripts of Anthocyanidin Reductase and Leucoanthocyanidin Reductase and Measurement of Catechin and Epicatechin in Tartary Buckwheat |
| title_fullStr | Transcripts of Anthocyanidin Reductase and Leucoanthocyanidin Reductase and Measurement of Catechin and Epicatechin in Tartary Buckwheat |
| title_full_unstemmed | Transcripts of Anthocyanidin Reductase and Leucoanthocyanidin Reductase and Measurement of Catechin and Epicatechin in Tartary Buckwheat |
| title_short | Transcripts of Anthocyanidin Reductase and Leucoanthocyanidin Reductase and Measurement of Catechin and Epicatechin in Tartary Buckwheat |
| title_sort | transcripts of anthocyanidin reductase and leucoanthocyanidin reductase and measurement of catechin and epicatechin in tartary buckwheat |
| url | http://dx.doi.org/10.1155/2014/726567 |
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