Lingguizhugan Decoction Protects against High-Fat-Diet-Induced Nonalcoholic Fatty Liver Disease by Alleviating Oxidative Stress and Activating Cholesterol Secretion
Background. Nonalcoholic fatty liver disease (NAFLD) has become a leading cause of liver transplantation. Lingguizhugan decoction (LGZG), a classical Chinese herbal formula, has beneficial effects on NAFLD animal models. Our study examined the impact of LGZG on hepatic global transcriptome of high-f...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2017/2790864 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832561767695253504 |
|---|---|
| author | Lili Yang Weili Lin Colleen A. Nugent Shijun Hao Haiyan Song Tao Liu Peiyong Zheng |
| author_facet | Lili Yang Weili Lin Colleen A. Nugent Shijun Hao Haiyan Song Tao Liu Peiyong Zheng |
| author_sort | Lili Yang |
| collection | DOAJ |
| description | Background. Nonalcoholic fatty liver disease (NAFLD) has become a leading cause of liver transplantation. Lingguizhugan decoction (LGZG), a classical Chinese herbal formula, has beneficial effects on NAFLD animal models. Our study examined the impact of LGZG on hepatic global transcriptome of high-fat-diet-induced NAFLD rats. Methods. Three groups of Wistar rats were included: normal, NAFLD model, and LGZG-treated NAFLD groups. Four weeks for the treatment, liver tissues were harvested for RNA sequencing. Differentially expressed genes (DEGs) and enriched pathways were detected on hepatic global transcriptome profile. Real-time PCR validated the regulatory patterns of LGZG on NAFLD rats. Results. DEGs between the NAFLD model and normal groups indicated the elevated peroxisome proliferator-activated receptor (PPAR) and hedgehog signaling pathways in NAFLD rats. In bile secretion pathway, genes involved in cholesterol secretion were activated by LGZG treatment. Increased expression of antioxidant OSIGN1 and decreased expression of genes (AHR, IRF2BP2, and RASGEF1B) that induce oxidative stress and inflammation were observed in NAFLD rats treated with LGZG. The regulatory patterns of LGZG treatment on these oxidative stress-related genes were confirmed by real-time PCR. Conclusion. Our study revealed a “two-hits-targeting” mechanism of LGZG in the treatment for NAFLD: alleviating oxidative stress and activating cholesterol secretion. |
| format | Article |
| id | doaj-art-5cf4435876d94f67b6c9b824efe65179 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-5cf4435876d94f67b6c9b824efe651792025-02-03T01:24:16ZengWileyInternational Journal of Genomics2314-436X2314-43782017-01-01201710.1155/2017/27908642790864Lingguizhugan Decoction Protects against High-Fat-Diet-Induced Nonalcoholic Fatty Liver Disease by Alleviating Oxidative Stress and Activating Cholesterol SecretionLili Yang0Weili Lin1Colleen A. Nugent2Shijun Hao3Haiyan Song4Tao Liu5Peiyong Zheng6Institute of Digestive Diseases, Longhua Hospital, China-Canada Center of Research for Digestive Diseases (ccCRDD), Shanghai University of Traditional Chinese Medicine, Shanghai 200032, ChinaKey Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, ChinaDigestive Diseases and Nutrition Center, Women and Children’s Hospital of Buffalo, Department of Pediatrics, The State University of New York at Buffalo, Buffalo, NY 14214, USAInstitute of Digestive Diseases, Longhua Hospital, China-Canada Center of Research for Digestive Diseases (ccCRDD), Shanghai University of Traditional Chinese Medicine, Shanghai 200032, ChinaInstitute of Digestive Diseases, Longhua Hospital, China-Canada Center of Research for Digestive Diseases (ccCRDD), Shanghai University of Traditional Chinese Medicine, Shanghai 200032, ChinaInstitute of Digestive Diseases, Longhua Hospital, China-Canada Center of Research for Digestive Diseases (ccCRDD), Shanghai University of Traditional Chinese Medicine, Shanghai 200032, ChinaInstitute of Digestive Diseases, Longhua Hospital, China-Canada Center of Research for Digestive Diseases (ccCRDD), Shanghai University of Traditional Chinese Medicine, Shanghai 200032, ChinaBackground. Nonalcoholic fatty liver disease (NAFLD) has become a leading cause of liver transplantation. Lingguizhugan decoction (LGZG), a classical Chinese herbal formula, has beneficial effects on NAFLD animal models. Our study examined the impact of LGZG on hepatic global transcriptome of high-fat-diet-induced NAFLD rats. Methods. Three groups of Wistar rats were included: normal, NAFLD model, and LGZG-treated NAFLD groups. Four weeks for the treatment, liver tissues were harvested for RNA sequencing. Differentially expressed genes (DEGs) and enriched pathways were detected on hepatic global transcriptome profile. Real-time PCR validated the regulatory patterns of LGZG on NAFLD rats. Results. DEGs between the NAFLD model and normal groups indicated the elevated peroxisome proliferator-activated receptor (PPAR) and hedgehog signaling pathways in NAFLD rats. In bile secretion pathway, genes involved in cholesterol secretion were activated by LGZG treatment. Increased expression of antioxidant OSIGN1 and decreased expression of genes (AHR, IRF2BP2, and RASGEF1B) that induce oxidative stress and inflammation were observed in NAFLD rats treated with LGZG. The regulatory patterns of LGZG treatment on these oxidative stress-related genes were confirmed by real-time PCR. Conclusion. Our study revealed a “two-hits-targeting” mechanism of LGZG in the treatment for NAFLD: alleviating oxidative stress and activating cholesterol secretion.http://dx.doi.org/10.1155/2017/2790864 |
| spellingShingle | Lili Yang Weili Lin Colleen A. Nugent Shijun Hao Haiyan Song Tao Liu Peiyong Zheng Lingguizhugan Decoction Protects against High-Fat-Diet-Induced Nonalcoholic Fatty Liver Disease by Alleviating Oxidative Stress and Activating Cholesterol Secretion International Journal of Genomics |
| title | Lingguizhugan Decoction Protects against High-Fat-Diet-Induced Nonalcoholic Fatty Liver Disease by Alleviating Oxidative Stress and Activating Cholesterol Secretion |
| title_full | Lingguizhugan Decoction Protects against High-Fat-Diet-Induced Nonalcoholic Fatty Liver Disease by Alleviating Oxidative Stress and Activating Cholesterol Secretion |
| title_fullStr | Lingguizhugan Decoction Protects against High-Fat-Diet-Induced Nonalcoholic Fatty Liver Disease by Alleviating Oxidative Stress and Activating Cholesterol Secretion |
| title_full_unstemmed | Lingguizhugan Decoction Protects against High-Fat-Diet-Induced Nonalcoholic Fatty Liver Disease by Alleviating Oxidative Stress and Activating Cholesterol Secretion |
| title_short | Lingguizhugan Decoction Protects against High-Fat-Diet-Induced Nonalcoholic Fatty Liver Disease by Alleviating Oxidative Stress and Activating Cholesterol Secretion |
| title_sort | lingguizhugan decoction protects against high fat diet induced nonalcoholic fatty liver disease by alleviating oxidative stress and activating cholesterol secretion |
| url | http://dx.doi.org/10.1155/2017/2790864 |
| work_keys_str_mv | AT liliyang lingguizhugandecoctionprotectsagainsthighfatdietinducednonalcoholicfattyliverdiseasebyalleviatingoxidativestressandactivatingcholesterolsecretion AT weililin lingguizhugandecoctionprotectsagainsthighfatdietinducednonalcoholicfattyliverdiseasebyalleviatingoxidativestressandactivatingcholesterolsecretion AT colleenanugent lingguizhugandecoctionprotectsagainsthighfatdietinducednonalcoholicfattyliverdiseasebyalleviatingoxidativestressandactivatingcholesterolsecretion AT shijunhao lingguizhugandecoctionprotectsagainsthighfatdietinducednonalcoholicfattyliverdiseasebyalleviatingoxidativestressandactivatingcholesterolsecretion AT haiyansong lingguizhugandecoctionprotectsagainsthighfatdietinducednonalcoholicfattyliverdiseasebyalleviatingoxidativestressandactivatingcholesterolsecretion AT taoliu lingguizhugandecoctionprotectsagainsthighfatdietinducednonalcoholicfattyliverdiseasebyalleviatingoxidativestressandactivatingcholesterolsecretion AT peiyongzheng lingguizhugandecoctionprotectsagainsthighfatdietinducednonalcoholicfattyliverdiseasebyalleviatingoxidativestressandactivatingcholesterolsecretion |