METABOLITIC COMPLEX OF LACTOBACTERIA INFECTED WITH MULTI-RESISTANT STRAINS LELLIOTTIA AMNIGENA WOUNDS IN CONDITIONS IN VIVO
Aim. To study the antimicrobial activity of lactobacilli metabolites in order to create alternative antimicrobial agents with multifunctional activity. Materials and methods. Skin wounds infected with a multiresistant strain of Lelliottia amnigena were modeled on 12 guinea pigs, divided into 4 gr...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
State Institution of Science «Research and Practical Center of Preventive and Clinical Medicine» State Administrative Department
2025-04-01
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| Series: | Клінічна та профілактична медицина |
| Subjects: | |
| Online Access: | https://cp-medical.com/index.php/journal/article/view/538 |
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| Summary: | Aim. To study the antimicrobial activity of lactobacilli metabolites in order to create alternative antimicrobial agents with multifunctional activity.
Materials and methods. Skin wounds infected with a multiresistant strain of Lelliottia amnigena were modeled on 12 guinea pigs, divided into 4 groups of 3 animals each. A 0.9% sodium chloride solution was applied to the control group, ciprofloxacin (A, Ananta Medicare, Great Britain) or a metabolite complex (L I, obtained by cultivating Lactobacillus rhamnosus GG microbial cells in their own ultrasonic disintegrates) to the therapeutic group, to the preventive and therapeutic group (Р) – additionally (before infection) a metabolic complex.
Results and discussion. Lower indicators of the number of microbial cells of L. amnigena were established in the prophylactic-treatment group (P) compared to the treatment groups (А and L I) (by ~ 4.2 times; p=0.04) on the eighth day of the study. There was no significant difference in wound seeding between ML and ciprofloxacin (p=0.1). The healing speed of experimental wounds exceeded the control wounds by 3.9–4.8 times. According to the planimetric indicators, there was also a decrease in wounds in groups P (0.3±0.03 cm²; p=0.03), A (0.67±0.1 cm2; p=0.03), L I (0, 9±0.2 cm²; p=0.005) relative to K (1.8±0.2 cm²) on the eighth day of the experiment. A faster reparative effect was established in group P compared to group A (р=0.01). Complete healing of infected wounds in group P occurred on the 11th day, which confirms the effectiveness of additional preliminary prophylactic application of ML.
Conclusions. The effectiveness of lactobacilli metabolites obtained by the author's method without the use of nutrient media was not inferior to that of the antibiotic. This indicates the possibility of promising application of ML for the construction of alternative antimicrobial agents of the new generation. |
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| ISSN: | 2616-4868 |