CD14loCD301b+ macrophages gathering as a proangiogenic marker in adipose tissues
The role of the monocyte marker CD14 in the regulation of obesity is increasingly recognized. Our observations indicated that Cd14−/− mice exhibited a leaner body shape compared to their wild-type (WT) counterparts. And the loss of CD14 alleviated high-fat diet–induced obesity in mice. In human subj...
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Elsevier
2025-01-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227524002256 |
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| author | Yibing Lv Yidan Zheng Shanshan Su Junyi Xiao Jie Yang Lingyun Xiong Yanyan Guo Xiaoqi Zhou Nengqiang Guo Ping Lei |
| author_facet | Yibing Lv Yidan Zheng Shanshan Su Junyi Xiao Jie Yang Lingyun Xiong Yanyan Guo Xiaoqi Zhou Nengqiang Guo Ping Lei |
| author_sort | Yibing Lv |
| collection | DOAJ |
| description | The role of the monocyte marker CD14 in the regulation of obesity is increasingly recognized. Our observations indicated that Cd14−/− mice exhibited a leaner body shape compared to their wild-type (WT) counterparts. And the loss of CD14 alleviated high-fat diet–induced obesity in mice. In human subjects, CD14 level was tested to be positively correlated with overweight and obesity. However, the relationship between CD14 and the development of obesity remains only partially understood. To investigate the underlying mechanisms, adipose tissues (ATs) from Cd14−/− and WT mice were subjected to deep RNA sequencing. Gene Ontology enrichment analysis revealed a significant enhancement of angiogenesis-related function in the Cd14−/− epididymal adipose tissues compared to WT counterpart, which was accompanied by an upregulation of Cd301b. Subsequent assays confirmed the enhanced angiogenesis and more accumulation of CD301b+ macrophages in Cd14−/− epididymal adipose tissues. Because Igf1 expression has been suggested to be associated with Cd301b expression through pseudotime analysis, we found it was insulin-like growth factor 1 secreted from Cd14−/− macrophages that mediated the angiogenesis enhancement. Collectively, our findings indicate that CD14 deficiency increased the accumulation of CD14loCD301b+ macrophages in ATs, which may serve as a proangiogenic marker, providing novel insights into the relationship between CD14 and obesity development. |
| format | Article |
| id | doaj-art-5cda3f5b92854697ac2c6ea527bee7df |
| institution | Kabale University |
| issn | 0022-2275 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj-art-5cda3f5b92854697ac2c6ea527bee7df2025-01-30T05:12:39ZengElsevierJournal of Lipid Research0022-22752025-01-01661100720CD14loCD301b+ macrophages gathering as a proangiogenic marker in adipose tissuesYibing Lv0Yidan Zheng1Shanshan Su2Junyi Xiao3Jie Yang4Lingyun Xiong5Yanyan Guo6Xiaoqi Zhou7Nengqiang Guo8Ping Lei9Department of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Henan Provincial Key Laboratory of Genetic Diseases and Functional Genomics, Medical Genetic Institute of Henan Province, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Transfusion Medicine, Wuhan Hospital of Traditional Chinese and Western Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; For correspondence: Nengqiang Guo; Ping LeiDepartment of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; For correspondence: Nengqiang Guo; Ping LeiThe role of the monocyte marker CD14 in the regulation of obesity is increasingly recognized. Our observations indicated that Cd14−/− mice exhibited a leaner body shape compared to their wild-type (WT) counterparts. And the loss of CD14 alleviated high-fat diet–induced obesity in mice. In human subjects, CD14 level was tested to be positively correlated with overweight and obesity. However, the relationship between CD14 and the development of obesity remains only partially understood. To investigate the underlying mechanisms, adipose tissues (ATs) from Cd14−/− and WT mice were subjected to deep RNA sequencing. Gene Ontology enrichment analysis revealed a significant enhancement of angiogenesis-related function in the Cd14−/− epididymal adipose tissues compared to WT counterpart, which was accompanied by an upregulation of Cd301b. Subsequent assays confirmed the enhanced angiogenesis and more accumulation of CD301b+ macrophages in Cd14−/− epididymal adipose tissues. Because Igf1 expression has been suggested to be associated with Cd301b expression through pseudotime analysis, we found it was insulin-like growth factor 1 secreted from Cd14−/− macrophages that mediated the angiogenesis enhancement. Collectively, our findings indicate that CD14 deficiency increased the accumulation of CD14loCD301b+ macrophages in ATs, which may serve as a proangiogenic marker, providing novel insights into the relationship between CD14 and obesity development.http://www.sciencedirect.com/science/article/pii/S0022227524002256angiogenesisCD14CD301bobesitymacrophages |
| spellingShingle | Yibing Lv Yidan Zheng Shanshan Su Junyi Xiao Jie Yang Lingyun Xiong Yanyan Guo Xiaoqi Zhou Nengqiang Guo Ping Lei CD14loCD301b+ macrophages gathering as a proangiogenic marker in adipose tissues Journal of Lipid Research angiogenesis CD14 CD301b obesity macrophages |
| title | CD14loCD301b+ macrophages gathering as a proangiogenic marker in adipose tissues |
| title_full | CD14loCD301b+ macrophages gathering as a proangiogenic marker in adipose tissues |
| title_fullStr | CD14loCD301b+ macrophages gathering as a proangiogenic marker in adipose tissues |
| title_full_unstemmed | CD14loCD301b+ macrophages gathering as a proangiogenic marker in adipose tissues |
| title_short | CD14loCD301b+ macrophages gathering as a proangiogenic marker in adipose tissues |
| title_sort | cd14locd301b macrophages gathering as a proangiogenic marker in adipose tissues |
| topic | angiogenesis CD14 CD301b obesity macrophages |
| url | http://www.sciencedirect.com/science/article/pii/S0022227524002256 |
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