Nasal Carriage of Methicillin-Resistant Staphylococcus aureus among Health Care Workers in Tertiary and Regional Hospitals in Dar es Salam, Tanzania
Methicillin-resistant Staphylococcus aureus (MRSA) among health care workers (HCWs) increases the risk of spreading the organism in hospital settings. A cross-sectional study was conducted between June and October 2016 among HCWs in tertiary and regional hospitals in Dar es Salaam, Tanzania, to dete...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2018/5058390 |
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| author | Agricola Joachim Sabrina J. Moyo Lillian Nkinda Mtebe Majigo Sima Rugarabamu Elizabeth G. Mkashabani Elia J. Mmbaga Naboth Mbembati Said Aboud Eligius F. Lyamuya |
| author_facet | Agricola Joachim Sabrina J. Moyo Lillian Nkinda Mtebe Majigo Sima Rugarabamu Elizabeth G. Mkashabani Elia J. Mmbaga Naboth Mbembati Said Aboud Eligius F. Lyamuya |
| author_sort | Agricola Joachim |
| collection | DOAJ |
| description | Methicillin-resistant Staphylococcus aureus (MRSA) among health care workers (HCWs) increases the risk of spreading the organism in hospital settings. A cross-sectional study was conducted between June and October 2016 among HCWs in tertiary and regional hospitals in Dar es Salaam, Tanzania, to determine the MRSA nasal carriage rate. Nasal swabs were collected from HCWs and cultured on mannitol salt agar. S. aureus was identified based on colonial morphology, Gram staining, catalase, coagulase, and DNase test results. MRSA was detected using the cefoxitin disk. Among 379 HCWs enrolled, 157/379 (41.4%) were colonized with S. aureus, of whom 59 (37.6%) were MRSA carriers giving an overall prevalence of 59/379 (15.6%). MRSA carriage was high among HCWs in Temeke (56.9%) and Amana (37.5%) regional hospitals. A high proportion of MRSA carriage was detected among nurses (35, 45.5%). MRSA isolates showed high resistance toward kanamycin (83.7%), gentamicin (83.1%), ciprofloxacin (71.2%), and trimethoprim-sulphamethoxazole (46.8%) compared to methicillin-sensitive S. aureus isolates (p≤0.001). In conclusion, we found a high nasal carriage of MRSA and resistance to commonly prescribed antimicrobial agents among HCWs. Implementation of infection control measures including contact precautions, urgent reporting of MRSA laboratory results, and routine MRSA screening of HCWs is highly needed to reduce MRSA spreading. |
| format | Article |
| id | doaj-art-5cbd4a326e6949769f668d039d17252d |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-5cbd4a326e6949769f668d039d17252d2025-02-03T01:25:24ZengWileyInternational Journal of Microbiology1687-918X1687-91982018-01-01201810.1155/2018/50583905058390Nasal Carriage of Methicillin-Resistant Staphylococcus aureus among Health Care Workers in Tertiary and Regional Hospitals in Dar es Salam, TanzaniaAgricola Joachim0Sabrina J. Moyo1Lillian Nkinda2Mtebe Majigo3Sima Rugarabamu4Elizabeth G. Mkashabani5Elia J. Mmbaga6Naboth Mbembati7Said Aboud8Eligius F. Lyamuya9Department of Microbiology and Immunology, Muhimbili University of Health and Allied Sciences, Dar Es Salaam, TanzaniaDepartment of Microbiology and Immunology, Muhimbili University of Health and Allied Sciences, Dar Es Salaam, TanzaniaDepartment of Microbiology and Immunology, Muhimbili University of Health and Allied Sciences, Dar Es Salaam, TanzaniaDepartment of Microbiology and Immunology, Muhimbili University of Health and Allied Sciences, Dar Es Salaam, TanzaniaDepartment of Microbiology and Immunology, Muhimbili University of Health and Allied Sciences, Dar Es Salaam, TanzaniaDepartment of Microbiology and Immunology, Muhimbili University of Health and Allied Sciences, Dar Es Salaam, TanzaniaDepartment of Epidemiology and Biostatistics, Muhimbili University of Health and Allied Sciences, Dar Es Salaam, TanzaniaDepartment of Surgery, Muhimbili University of Health and Allied Sciences, Dar Es Salaam, TanzaniaDepartment of Microbiology and Immunology, Muhimbili University of Health and Allied Sciences, Dar Es Salaam, TanzaniaDepartment of Microbiology and Immunology, Muhimbili University of Health and Allied Sciences, Dar Es Salaam, TanzaniaMethicillin-resistant Staphylococcus aureus (MRSA) among health care workers (HCWs) increases the risk of spreading the organism in hospital settings. A cross-sectional study was conducted between June and October 2016 among HCWs in tertiary and regional hospitals in Dar es Salaam, Tanzania, to determine the MRSA nasal carriage rate. Nasal swabs were collected from HCWs and cultured on mannitol salt agar. S. aureus was identified based on colonial morphology, Gram staining, catalase, coagulase, and DNase test results. MRSA was detected using the cefoxitin disk. Among 379 HCWs enrolled, 157/379 (41.4%) were colonized with S. aureus, of whom 59 (37.6%) were MRSA carriers giving an overall prevalence of 59/379 (15.6%). MRSA carriage was high among HCWs in Temeke (56.9%) and Amana (37.5%) regional hospitals. A high proportion of MRSA carriage was detected among nurses (35, 45.5%). MRSA isolates showed high resistance toward kanamycin (83.7%), gentamicin (83.1%), ciprofloxacin (71.2%), and trimethoprim-sulphamethoxazole (46.8%) compared to methicillin-sensitive S. aureus isolates (p≤0.001). In conclusion, we found a high nasal carriage of MRSA and resistance to commonly prescribed antimicrobial agents among HCWs. Implementation of infection control measures including contact precautions, urgent reporting of MRSA laboratory results, and routine MRSA screening of HCWs is highly needed to reduce MRSA spreading.http://dx.doi.org/10.1155/2018/5058390 |
| spellingShingle | Agricola Joachim Sabrina J. Moyo Lillian Nkinda Mtebe Majigo Sima Rugarabamu Elizabeth G. Mkashabani Elia J. Mmbaga Naboth Mbembati Said Aboud Eligius F. Lyamuya Nasal Carriage of Methicillin-Resistant Staphylococcus aureus among Health Care Workers in Tertiary and Regional Hospitals in Dar es Salam, Tanzania International Journal of Microbiology |
| title | Nasal Carriage of Methicillin-Resistant Staphylococcus aureus among Health Care Workers in Tertiary and Regional Hospitals in Dar es Salam, Tanzania |
| title_full | Nasal Carriage of Methicillin-Resistant Staphylococcus aureus among Health Care Workers in Tertiary and Regional Hospitals in Dar es Salam, Tanzania |
| title_fullStr | Nasal Carriage of Methicillin-Resistant Staphylococcus aureus among Health Care Workers in Tertiary and Regional Hospitals in Dar es Salam, Tanzania |
| title_full_unstemmed | Nasal Carriage of Methicillin-Resistant Staphylococcus aureus among Health Care Workers in Tertiary and Regional Hospitals in Dar es Salam, Tanzania |
| title_short | Nasal Carriage of Methicillin-Resistant Staphylococcus aureus among Health Care Workers in Tertiary and Regional Hospitals in Dar es Salam, Tanzania |
| title_sort | nasal carriage of methicillin resistant staphylococcus aureus among health care workers in tertiary and regional hospitals in dar es salam tanzania |
| url | http://dx.doi.org/10.1155/2018/5058390 |
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