USE OF REAL-TIME qRT-PCR FOR FMD VIRUS DETECTION IN CATTLE MILK
FMDV can be shed with milk of infected animals and infect susceptible animals. The possibility of using real-time qRT-PCR for FMD virus detection in cattle milk was assessed. To prepare samples of FMDV infected milk the following strains were used: А No. 2177/ Amursky/2013, О No. 2123/South Ossetia/...
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Da Vinci Media
2019-04-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/397 |
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| author | A. M. Timina S. N. Fomina Т. К. Mayorova |
| author_facet | A. M. Timina S. N. Fomina Т. К. Mayorova |
| author_sort | A. M. Timina |
| collection | DOAJ |
| description | FMDV can be shed with milk of infected animals and infect susceptible animals. The possibility of using real-time qRT-PCR for FMD virus detection in cattle milk was assessed. To prepare samples of FMDV infected milk the following strains were used: А No. 2177/ Amursky/2013, О No. 2123/South Ossetia/2011, Asia-1 No. 1987/Amursky/2005, С No. 564, SAT-1 No. 2033/Botswana, SAT-2/Saudi Arabia, SAT-3/Bech 1/65. For specifcity test swine vesicular disease O-72 strain, Vesicular Exanthema of Swine A-48 strain and vesicular stomatitis virus Indiana strain from FGBI “ARRIAH” collection were used. The obtained data showed that real time qRT-PCR can be successfully used for the virus detection in milk of infected cows as well as in epithelium and cell cultures. Analytical sensitivity of real-time qRT-PCR in milk samples is 1–10 TCD50/ml, the method specifcity is 100%. When identifying basic validation characteristics of the method it was proven that real-time qRT-PCR demonstrates precision and sensitivity and can detect FMDV in its genetic diversity. So, the detected data showed that the real-time qRT-PCR is a precious method of milk and dairy product control. |
| format | Article |
| id | doaj-art-5cba3cf3328944bc8834c809e5ab0efd |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2019-04-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-5cba3cf3328944bc8834c809e5ab0efd2025-02-06T09:52:07ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592019-04-0101222810.29326/2304-196X-2019-1-28-22-25393USE OF REAL-TIME qRT-PCR FOR FMD VIRUS DETECTION IN CATTLE MILKA. M. Timina0S. N. Fomina1Т. К. Mayorova2FGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFMDV can be shed with milk of infected animals and infect susceptible animals. The possibility of using real-time qRT-PCR for FMD virus detection in cattle milk was assessed. To prepare samples of FMDV infected milk the following strains were used: А No. 2177/ Amursky/2013, О No. 2123/South Ossetia/2011, Asia-1 No. 1987/Amursky/2005, С No. 564, SAT-1 No. 2033/Botswana, SAT-2/Saudi Arabia, SAT-3/Bech 1/65. For specifcity test swine vesicular disease O-72 strain, Vesicular Exanthema of Swine A-48 strain and vesicular stomatitis virus Indiana strain from FGBI “ARRIAH” collection were used. The obtained data showed that real time qRT-PCR can be successfully used for the virus detection in milk of infected cows as well as in epithelium and cell cultures. Analytical sensitivity of real-time qRT-PCR in milk samples is 1–10 TCD50/ml, the method specifcity is 100%. When identifying basic validation characteristics of the method it was proven that real-time qRT-PCR demonstrates precision and sensitivity and can detect FMDV in its genetic diversity. So, the detected data showed that the real-time qRT-PCR is a precious method of milk and dairy product control.https://veterinary.arriah.ru/jour/article/view/397fmd virusmilkreal-time qrt-pcrvalidation |
| spellingShingle | A. M. Timina S. N. Fomina Т. К. Mayorova USE OF REAL-TIME qRT-PCR FOR FMD VIRUS DETECTION IN CATTLE MILK Ветеринария сегодня fmd virus milk real-time qrt-pcr validation |
| title | USE OF REAL-TIME qRT-PCR FOR FMD VIRUS DETECTION IN CATTLE MILK |
| title_full | USE OF REAL-TIME qRT-PCR FOR FMD VIRUS DETECTION IN CATTLE MILK |
| title_fullStr | USE OF REAL-TIME qRT-PCR FOR FMD VIRUS DETECTION IN CATTLE MILK |
| title_full_unstemmed | USE OF REAL-TIME qRT-PCR FOR FMD VIRUS DETECTION IN CATTLE MILK |
| title_short | USE OF REAL-TIME qRT-PCR FOR FMD VIRUS DETECTION IN CATTLE MILK |
| title_sort | use of real time qrt pcr for fmd virus detection in cattle milk |
| topic | fmd virus milk real-time qrt-pcr validation |
| url | https://veterinary.arriah.ru/jour/article/view/397 |
| work_keys_str_mv | AT amtimina useofrealtimeqrtpcrforfmdvirusdetectionincattlemilk AT snfomina useofrealtimeqrtpcrforfmdvirusdetectionincattlemilk AT tkmayorova useofrealtimeqrtpcrforfmdvirusdetectionincattlemilk |