Immobilized Saccharomyces cerevisiae viable cells for electrochemical biosensing of Cu(II)
Abstract Electrodes functionalised with weak electroactive microorganisms offer a viable alternative to conventional chemical sensors for detecting priority pollutants in bioremediation processes. Biofilm-based biosensors have been proposed for this purpose. However, biofilm formation and maturation...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-025-86702-8 |
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| author | Ehtisham Wahid Ohiemi Benjamin Ocheja Sunday Olakunle Oguntomi Run Pan Matteo Grattieri Nicoletta Guaragnella Cataldo Guaragnella Enrico Marsili |
| author_facet | Ehtisham Wahid Ohiemi Benjamin Ocheja Sunday Olakunle Oguntomi Run Pan Matteo Grattieri Nicoletta Guaragnella Cataldo Guaragnella Enrico Marsili |
| author_sort | Ehtisham Wahid |
| collection | DOAJ |
| description | Abstract Electrodes functionalised with weak electroactive microorganisms offer a viable alternative to conventional chemical sensors for detecting priority pollutants in bioremediation processes. Biofilm-based biosensors have been proposed for this purpose. However, biofilm formation and maturation require 24–48 h, and the microstructure and coverage of the electrode surface cannot be controlled, leading to poorly reproducible signal and sensitivity. Alternatively, semiconductive biocompatible coatings can be used for viable cell immobilization, achieving reproducible coverage and resulting in a stable biosensor response. In this work, we use a polydopamine (PDA)-based coating to immobilize Saccharomyces cerevisiae yeast viable cells on carbon screen printed electrodes (SPE) for Cu(II) detection, with potassium ferricyanide (K3[Fe (CN)6]) as a redox mediator. Under these conditions, the current output correlates with Cu (II) concentration, reaching a limit of detection of 2.2 µM, as calculated from the chronoamperometric response. The bioelectrochemical results are supported by standard viability assays, microscopy, and electrochemical impedance spectroscopy. The PDA coatings can be functionalised with different mutant strains, thus expanding the toolbox for biosensor design in bioremediation. |
| format | Article |
| id | doaj-art-5ca56bc9c32c404a952c342d1d584a56 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-5ca56bc9c32c404a952c342d1d584a562025-01-26T12:30:34ZengNature PortfolioScientific Reports2045-23222025-01-0115111110.1038/s41598-025-86702-8Immobilized Saccharomyces cerevisiae viable cells for electrochemical biosensing of Cu(II)Ehtisham Wahid0Ohiemi Benjamin Ocheja1Sunday Olakunle Oguntomi2Run Pan3Matteo Grattieri4Nicoletta Guaragnella5Cataldo Guaragnella6Enrico Marsili7DEI – Department of Electrical and Information Engineering, Polytechnic of BariDepartment of Biosciences, Biotechnology and Environment, University of BariNottingham Ningbo China Beacons of Excellence Research and Innovation Institute, University of Nottingham Ningbo ChinaNottingham Ningbo China Beacons of Excellence Research and Innovation Institute, University of Nottingham Ningbo ChinaDepartment of Chemistry, University of BariDepartment of Biosciences, Biotechnology and Environment, University of BariDEI – Department of Electrical and Information Engineering, Polytechnic of BariNottingham Ningbo China Beacons of Excellence Research and Innovation Institute, University of Nottingham Ningbo ChinaAbstract Electrodes functionalised with weak electroactive microorganisms offer a viable alternative to conventional chemical sensors for detecting priority pollutants in bioremediation processes. Biofilm-based biosensors have been proposed for this purpose. However, biofilm formation and maturation require 24–48 h, and the microstructure and coverage of the electrode surface cannot be controlled, leading to poorly reproducible signal and sensitivity. Alternatively, semiconductive biocompatible coatings can be used for viable cell immobilization, achieving reproducible coverage and resulting in a stable biosensor response. In this work, we use a polydopamine (PDA)-based coating to immobilize Saccharomyces cerevisiae yeast viable cells on carbon screen printed electrodes (SPE) for Cu(II) detection, with potassium ferricyanide (K3[Fe (CN)6]) as a redox mediator. Under these conditions, the current output correlates with Cu (II) concentration, reaching a limit of detection of 2.2 µM, as calculated from the chronoamperometric response. The bioelectrochemical results are supported by standard viability assays, microscopy, and electrochemical impedance spectroscopy. The PDA coatings can be functionalised with different mutant strains, thus expanding the toolbox for biosensor design in bioremediation.https://doi.org/10.1038/s41598-025-86702-8PolydopamineBiosensorsBioremediationSaccharomyces cerevisiaeExtracellular electron transfer |
| spellingShingle | Ehtisham Wahid Ohiemi Benjamin Ocheja Sunday Olakunle Oguntomi Run Pan Matteo Grattieri Nicoletta Guaragnella Cataldo Guaragnella Enrico Marsili Immobilized Saccharomyces cerevisiae viable cells for electrochemical biosensing of Cu(II) Scientific Reports Polydopamine Biosensors Bioremediation Saccharomyces cerevisiae Extracellular electron transfer |
| title | Immobilized Saccharomyces cerevisiae viable cells for electrochemical biosensing of Cu(II) |
| title_full | Immobilized Saccharomyces cerevisiae viable cells for electrochemical biosensing of Cu(II) |
| title_fullStr | Immobilized Saccharomyces cerevisiae viable cells for electrochemical biosensing of Cu(II) |
| title_full_unstemmed | Immobilized Saccharomyces cerevisiae viable cells for electrochemical biosensing of Cu(II) |
| title_short | Immobilized Saccharomyces cerevisiae viable cells for electrochemical biosensing of Cu(II) |
| title_sort | immobilized saccharomyces cerevisiae viable cells for electrochemical biosensing of cu ii |
| topic | Polydopamine Biosensors Bioremediation Saccharomyces cerevisiae Extracellular electron transfer |
| url | https://doi.org/10.1038/s41598-025-86702-8 |
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