Genome-Wide Identification of Superoxide Dismutase (<i>SOD</i>) Gene Family in <i>Cymbidium</i> Species and Functional Analysis of <i>CsSOD</i>s Under Salt Stress in <i>Cymbidium sinense</i>

Genome-Wide Identification of Superoxide Dismutase (<i>SOD</i>) Gene Family in <i>Cymbidium</i> Species and Functional Analysis of <i>CsSOD</i>s Under Salt Stress in <i>Cymbidium sinense</i>

Superoxide dismutase (SOD) enzymes are essential for reducing oxidative damage resulting from overabundant reactive oxygen species under abiotic stress. While the <i>SOD</i> gene family has been extensively studied in many species, research focusing on <i>Cymbidium</i> specie...

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Bibliographic Details
Main Authors: Ruyi Li, Songkun Lin, Yin Yan, Yuming Chen, Linying Wang, Yuzhen Zhou, Shuling Tang, Ning Liu
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Horticulturae
Subjects:
orchid
<i>Cymbidium sinense</i>
<i>SOD</i> gene family
salt stress
Online Access:https://www.mdpi.com/2311-7524/11/1/95
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Summary:Superoxide dismutase (SOD) enzymes are essential for reducing oxidative damage resulting from overabundant reactive oxygen species under abiotic stress. While the <i>SOD</i> gene family has been extensively studied in many species, research focusing on <i>Cymbidium</i> species remains limited. In this study, a comprehensive analysis of the <i>SOD</i> gene family in three <i>Cymbidium</i> genomes was conducted. A total of 23 <i>SOD</i> genes were identified, with nine <i>SOD</i>s in <i>C. sinense</i>, eight in <i>C. ensifolium</i>, and six in <i>C. goeringii</i>. These <i>SOD</i> genes were categorized into three clades: Cu/Zn-SOD, Fe-SOD, and Mn-SOD, with the Cu/Zn-SOD being the most abundant in these three types. This classification was supported by analyses of conserved domains, motifs, and phylogenetic relationships. <i>Cis</i>-element prediction showed that stress-responsive elements were identified in most <i>SOD</i>s. Transcriptomic data revealed that seven <i>CsSOD</i>s exhibited a border expression in all sequenced tissues, while two exhibited undetectable expression levels. Further qRT-PCR analysis showed that all <i>CsSOD</i>s were upregulated under salt stress, with some exhibiting significant changes in expression. These findings all highlight the crucial role of <i>CsSOD</i>s in the salt stress response and provide valuable insights for further breeding salt-tolerance varieties of <i>C. sinense</i>.
ISSN:2311-7524

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