Derivation of Two New Human Embryonic Stem Cell Lines from Nonviable Human Embryos
We report the derivation and characterization of two new human embryonic stem cells (hESC) lines (CU1 and CU2) from embryos with an irreversible loss of integrated organismic function. In addition, we analyzed retrospective data of morphological progression from embryonic day (ED) 5 to ED6 for 2480...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.4061/2011/765378 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832545851494367232 |
|---|---|
| author | Svetlana Gavrilov Darja Marolt Nataki C. Douglas Robert W. Prosser Imran Khalid Mark V. Sauer Donald W. Landry Gordana Vunjak-Novakovic Virginia E. Papaioannou |
| author_facet | Svetlana Gavrilov Darja Marolt Nataki C. Douglas Robert W. Prosser Imran Khalid Mark V. Sauer Donald W. Landry Gordana Vunjak-Novakovic Virginia E. Papaioannou |
| author_sort | Svetlana Gavrilov |
| collection | DOAJ |
| description | We report the derivation and characterization of two new human embryonic stem cells (hESC) lines (CU1 and CU2) from embryos with an irreversible loss of integrated organismic function. In addition, we analyzed retrospective data of morphological progression from embryonic day (ED) 5 to ED6 for 2480 embryos not suitable for clinical use to assess grading criteria indicative of loss of viability on ED5. Our analysis indicated that a large proportion of in vitro fertilization (IVF) embryos not suitable for clinical use could be used for hESC derivation. Based on these combined findings, we propose that criteria commonly used in IVF clinics to determine optimal embryos for uterine transfer can be employed to predict the potential for hESC derivation from poor quality embryos without the destruction of vital human embryos. |
| format | Article |
| id | doaj-art-5c3c130cebf24fc89935f6afa2c9b3dc |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-5c3c130cebf24fc89935f6afa2c9b3dc2025-02-03T07:24:25ZengWileyStem Cells International1687-966X1687-96782011-01-01201110.4061/2011/765378765378Derivation of Two New Human Embryonic Stem Cell Lines from Nonviable Human EmbryosSvetlana Gavrilov0Darja Marolt1Nataki C. Douglas2Robert W. Prosser3Imran Khalid4Mark V. Sauer5Donald W. Landry6Gordana Vunjak-Novakovic7Virginia E. Papaioannou8Department of Genetics and Development, College of Physicians and Surgeons of Columbia University, 701 West 168th Street, New York, NY 10032, USADepartment of Biomedical Engineering, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USADepartment of Obstetrics and Gynecology, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USADepartment of Obstetrics and Gynecology, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USADivision of Experimental Therapeutics, Department of Medicine, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USADepartment of Obstetrics and Gynecology, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USADivision of Experimental Therapeutics, Department of Medicine, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USADepartment of Biomedical Engineering, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USADepartment of Genetics and Development, College of Physicians and Surgeons of Columbia University, 701 West 168th Street, New York, NY 10032, USAWe report the derivation and characterization of two new human embryonic stem cells (hESC) lines (CU1 and CU2) from embryos with an irreversible loss of integrated organismic function. In addition, we analyzed retrospective data of morphological progression from embryonic day (ED) 5 to ED6 for 2480 embryos not suitable for clinical use to assess grading criteria indicative of loss of viability on ED5. Our analysis indicated that a large proportion of in vitro fertilization (IVF) embryos not suitable for clinical use could be used for hESC derivation. Based on these combined findings, we propose that criteria commonly used in IVF clinics to determine optimal embryos for uterine transfer can be employed to predict the potential for hESC derivation from poor quality embryos without the destruction of vital human embryos.http://dx.doi.org/10.4061/2011/765378 |
| spellingShingle | Svetlana Gavrilov Darja Marolt Nataki C. Douglas Robert W. Prosser Imran Khalid Mark V. Sauer Donald W. Landry Gordana Vunjak-Novakovic Virginia E. Papaioannou Derivation of Two New Human Embryonic Stem Cell Lines from Nonviable Human Embryos Stem Cells International |
| title | Derivation of Two New Human Embryonic Stem Cell Lines from Nonviable Human Embryos |
| title_full | Derivation of Two New Human Embryonic Stem Cell Lines from Nonviable Human Embryos |
| title_fullStr | Derivation of Two New Human Embryonic Stem Cell Lines from Nonviable Human Embryos |
| title_full_unstemmed | Derivation of Two New Human Embryonic Stem Cell Lines from Nonviable Human Embryos |
| title_short | Derivation of Two New Human Embryonic Stem Cell Lines from Nonviable Human Embryos |
| title_sort | derivation of two new human embryonic stem cell lines from nonviable human embryos |
| url | http://dx.doi.org/10.4061/2011/765378 |
| work_keys_str_mv | AT svetlanagavrilov derivationoftwonewhumanembryonicstemcelllinesfromnonviablehumanembryos AT darjamarolt derivationoftwonewhumanembryonicstemcelllinesfromnonviablehumanembryos AT natakicdouglas derivationoftwonewhumanembryonicstemcelllinesfromnonviablehumanembryos AT robertwprosser derivationoftwonewhumanembryonicstemcelllinesfromnonviablehumanembryos AT imrankhalid derivationoftwonewhumanembryonicstemcelllinesfromnonviablehumanembryos AT markvsauer derivationoftwonewhumanembryonicstemcelllinesfromnonviablehumanembryos AT donaldwlandry derivationoftwonewhumanembryonicstemcelllinesfromnonviablehumanembryos AT gordanavunjaknovakovic derivationoftwonewhumanembryonicstemcelllinesfromnonviablehumanembryos AT virginiaepapaioannou derivationoftwonewhumanembryonicstemcelllinesfromnonviablehumanembryos |