E. coli-Produced BMP-2 as a Chemopreventive Strategy for Colon Cancer: A Proof-of-Concept Study
Colon cancer is a serious health problem, and novel preventive and therapeutical avenues are urgently called for. Delivery of proteins with anticancer activity through genetically modified bacteria provides an interesting, potentially specific, economic and effective approach here. Interestingly, bo...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2012/895462 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558657100840960 |
|---|---|
| author | Saravanan Yuvaraj Sa’ad H. Al-Lahham Rajesh Somasundaram Patrick A. Figaroa Maikel P. Peppelenbosch Nicolaas A. Bos |
| author_facet | Saravanan Yuvaraj Sa’ad H. Al-Lahham Rajesh Somasundaram Patrick A. Figaroa Maikel P. Peppelenbosch Nicolaas A. Bos |
| author_sort | Saravanan Yuvaraj |
| collection | DOAJ |
| description | Colon cancer is a serious health problem, and novel preventive and therapeutical avenues are urgently called for. Delivery of proteins with anticancer activity through genetically modified bacteria provides an interesting, potentially specific, economic and effective approach here. Interestingly, bone morphogenetic protein 2 (BMP-2) is an important and powerful tumour suppressor in the colon and is thus an attractive candidate protein for delivery through genetically modified bacteria. It has not been shown, however, that BMP production in the bacterial context is effective on colon cancer cells. Here we demonstrate that transforming E. coli with a cDNA encoding an ileal-derived mature human BMP-2 induces effective apoptosis in an in vitro model system for colorectal cancer, whereas the maternal organism was not effective in this respect. Furthermore, these effects were sensitive to cotreatment with the BMP inhibitor Noggin. We propose that prevention and treatment of colorectal cancer using transgenic bacteria is feasible. |
| format | Article |
| id | doaj-art-5c3a035ef2a44593b1050c19517e233f |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-5c3a035ef2a44593b1050c19517e233f2025-02-03T01:31:48ZengWileyGastroenterology Research and Practice1687-61211687-630X2012-01-01201210.1155/2012/895462895462E. coli-Produced BMP-2 as a Chemopreventive Strategy for Colon Cancer: A Proof-of-Concept StudySaravanan Yuvaraj0Sa’ad H. Al-Lahham1Rajesh Somasundaram2Patrick A. Figaroa3Maikel P. Peppelenbosch4Nicolaas A. Bos5Department of Cell Biology, University Medical Center Groningen, University of Groningen, A. Deusinglaan 1, 9713 AV Groningen, The NetherlandsDepartment of Cell Biology, University Medical Center Groningen, University of Groningen, A. Deusinglaan 1, 9713 AV Groningen, The NetherlandsDepartment of Cell Biology, University Medical Center Groningen, University of Groningen, A. Deusinglaan 1, 9713 AV Groningen, The NetherlandsDepartment of Cell Biology, University Medical Center Groningen, University of Groningen, A. Deusinglaan 1, 9713 AV Groningen, The NetherlandsDepartment of Cell Biology, University Medical Center Groningen, University of Groningen, A. Deusinglaan 1, 9713 AV Groningen, The NetherlandsDepartment of Cell Biology, University Medical Center Groningen, University of Groningen, A. Deusinglaan 1, 9713 AV Groningen, The NetherlandsColon cancer is a serious health problem, and novel preventive and therapeutical avenues are urgently called for. Delivery of proteins with anticancer activity through genetically modified bacteria provides an interesting, potentially specific, economic and effective approach here. Interestingly, bone morphogenetic protein 2 (BMP-2) is an important and powerful tumour suppressor in the colon and is thus an attractive candidate protein for delivery through genetically modified bacteria. It has not been shown, however, that BMP production in the bacterial context is effective on colon cancer cells. Here we demonstrate that transforming E. coli with a cDNA encoding an ileal-derived mature human BMP-2 induces effective apoptosis in an in vitro model system for colorectal cancer, whereas the maternal organism was not effective in this respect. Furthermore, these effects were sensitive to cotreatment with the BMP inhibitor Noggin. We propose that prevention and treatment of colorectal cancer using transgenic bacteria is feasible.http://dx.doi.org/10.1155/2012/895462 |
| spellingShingle | Saravanan Yuvaraj Sa’ad H. Al-Lahham Rajesh Somasundaram Patrick A. Figaroa Maikel P. Peppelenbosch Nicolaas A. Bos E. coli-Produced BMP-2 as a Chemopreventive Strategy for Colon Cancer: A Proof-of-Concept Study Gastroenterology Research and Practice |
| title | E. coli-Produced BMP-2 as a Chemopreventive Strategy for Colon Cancer: A Proof-of-Concept Study |
| title_full | E. coli-Produced BMP-2 as a Chemopreventive Strategy for Colon Cancer: A Proof-of-Concept Study |
| title_fullStr | E. coli-Produced BMP-2 as a Chemopreventive Strategy for Colon Cancer: A Proof-of-Concept Study |
| title_full_unstemmed | E. coli-Produced BMP-2 as a Chemopreventive Strategy for Colon Cancer: A Proof-of-Concept Study |
| title_short | E. coli-Produced BMP-2 as a Chemopreventive Strategy for Colon Cancer: A Proof-of-Concept Study |
| title_sort | e coli produced bmp 2 as a chemopreventive strategy for colon cancer a proof of concept study |
| url | http://dx.doi.org/10.1155/2012/895462 |
| work_keys_str_mv | AT saravananyuvaraj ecoliproducedbmp2asachemopreventivestrategyforcoloncanceraproofofconceptstudy AT saadhallahham ecoliproducedbmp2asachemopreventivestrategyforcoloncanceraproofofconceptstudy AT rajeshsomasundaram ecoliproducedbmp2asachemopreventivestrategyforcoloncanceraproofofconceptstudy AT patrickafigaroa ecoliproducedbmp2asachemopreventivestrategyforcoloncanceraproofofconceptstudy AT maikelppeppelenbosch ecoliproducedbmp2asachemopreventivestrategyforcoloncanceraproofofconceptstudy AT nicolaasabos ecoliproducedbmp2asachemopreventivestrategyforcoloncanceraproofofconceptstudy |