Comparing monolithic and fused core HPLC columns for fast chromatographic analysis of fat-soluble vitamins
HPLC stationary phases of monolithic and fused core type can be used to achieve fast chromatographic separation as an alternative to UPLC. In this study, monolithic and fused core stationary phases are compared for fast separation of four fat-soluble vitamins. Three new methods on the first and seco...
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| Format: | Article |
| Language: | English |
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Sciendo
2017-06-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.1515/acph-2017-0013 |
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| author | Kurdi Said El Muaileq Dina Abu Alhazmi Hassan A. Bratty Mohammed Al Deeb Sami El |
| author_facet | Kurdi Said El Muaileq Dina Abu Alhazmi Hassan A. Bratty Mohammed Al Deeb Sami El |
| author_sort | Kurdi Said El |
| collection | DOAJ |
| description | HPLC stationary phases of monolithic and fused core type can be used to achieve fast chromatographic separation as an alternative to UPLC. In this study, monolithic and fused core stationary phases are compared for fast separation of four fat-soluble vitamins. Three new methods on the first and second generation monolithic silica RP-18e columns and a fused core pentafluoro-phenyl propyl column were developed. Application of three fused core columns offered comparable separations of retinyl palmitate, DL-α-tocopheryl acetate, cholecalciferol and menadione in terms of elution speed and separation efficiency. Separation was achieved in approx. 5 min with good resolution (Rs > 5) and precision (RSD ≤ 0.6 %). Monolithic columns showed, however, a higher number of theoretical plates, better precision and lower column backpressure than the fused core column. The three developed methods were successfully applied to separate and quantitate fat-soluble vitamins in commercial products. |
| format | Article |
| id | doaj-art-5c18235d38424548964478143a2822e3 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2017-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-5c18235d38424548964478143a2822e32025-02-02T11:50:20ZengSciendoActa Pharmaceutica1846-95582017-06-0167220321310.1515/acph-2017-0013acph-2017-0013Comparing monolithic and fused core HPLC columns for fast chromatographic analysis of fat-soluble vitaminsKurdi Said El0Muaileq Dina Abu1Alhazmi Hassan A.2Bratty Mohammed Al3Deeb Sami El4Chemistry Department Faculty of Science, Islamic University Gaza, Gaza, Palestine, State ofPharmaceutical Chemistry Department, Faculty of Pharmacy Al-Azhar University-Gaza, Gaza, Palestine, State ofPharmaceutical Chemistry Department, Faculty of Pharmacy Jazan University, Jazan, Saudi ArabiaPharmaceutical Chemistry Department, Faculty of Pharmacy Jazan University, Jazan, Saudi ArabiaInstitute of Medicinal and Pharmaceutical Chemistry TU Braunschweig, D-38106 Braunschweig, GermanyHPLC stationary phases of monolithic and fused core type can be used to achieve fast chromatographic separation as an alternative to UPLC. In this study, monolithic and fused core stationary phases are compared for fast separation of four fat-soluble vitamins. Three new methods on the first and second generation monolithic silica RP-18e columns and a fused core pentafluoro-phenyl propyl column were developed. Application of three fused core columns offered comparable separations of retinyl palmitate, DL-α-tocopheryl acetate, cholecalciferol and menadione in terms of elution speed and separation efficiency. Separation was achieved in approx. 5 min with good resolution (Rs > 5) and precision (RSD ≤ 0.6 %). Monolithic columns showed, however, a higher number of theoretical plates, better precision and lower column backpressure than the fused core column. The three developed methods were successfully applied to separate and quantitate fat-soluble vitamins in commercial products.https://doi.org/10.1515/acph-2017-0013monolithic silica columnfused core columnhplcfat-soluble vitaminsanalytical performance |
| spellingShingle | Kurdi Said El Muaileq Dina Abu Alhazmi Hassan A. Bratty Mohammed Al Deeb Sami El Comparing monolithic and fused core HPLC columns for fast chromatographic analysis of fat-soluble vitamins Acta Pharmaceutica monolithic silica column fused core column hplc fat-soluble vitamins analytical performance |
| title | Comparing monolithic and fused core HPLC columns for fast chromatographic analysis of fat-soluble vitamins |
| title_full | Comparing monolithic and fused core HPLC columns for fast chromatographic analysis of fat-soluble vitamins |
| title_fullStr | Comparing monolithic and fused core HPLC columns for fast chromatographic analysis of fat-soluble vitamins |
| title_full_unstemmed | Comparing monolithic and fused core HPLC columns for fast chromatographic analysis of fat-soluble vitamins |
| title_short | Comparing monolithic and fused core HPLC columns for fast chromatographic analysis of fat-soluble vitamins |
| title_sort | comparing monolithic and fused core hplc columns for fast chromatographic analysis of fat soluble vitamins |
| topic | monolithic silica column fused core column hplc fat-soluble vitamins analytical performance |
| url | https://doi.org/10.1515/acph-2017-0013 |
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