Detection of Vibrio harveyi using hemolysin primer in tiger shrimp Penaeus monodon
ABSTRACT This study was aimed to analyze the sensitivity and ability of primer hemolysin in detecting pathogenetic Vibrio on tiger shrimp post-larvae (PL) exposed under different exposure times in media inoculated with Vibrio harveyi. The PL of tiger shrimp were infected with 106 cfu/mL of V. harv...
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Asosiasi Sains Akuakultur Indonesia
2015-05-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/jai/article/view/9362 |
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| author | Irma Suriyani Ince Ayu Khairana Kadriah ilmiah Kuruseng |
| author_facet | Irma Suriyani Ince Ayu Khairana Kadriah ilmiah Kuruseng |
| author_sort | Irma Suriyani |
| collection | DOAJ |
| description | ABSTRACT
This study was aimed to analyze the sensitivity and ability of primer hemolysin in detecting pathogenetic Vibrio on tiger shrimp post-larvae (PL) exposed under different exposure times in media inoculated with Vibrio harveyi. The PL of tiger shrimp were infected with 106 cfu/mL of V. harveyi by immersion method for three, six, 12, 24, 48 and 72 hours. The presence of hemolisin genes was detected by PCR techniques. The electrophoresis detected narrow hemolysin genes after PL were exposed for three and six hours. Clear visible bands of DNA Vibrio were observed for 12 hours of exposure. In contrast, no detected hemolysin gene of Vibrio was observed for PL exposed within 24, 48, and 72 hours. The rapid detection on Vibrio pathogenic for tiger shrimp PL should be conducted within three to 12 hours of exposure. No recommendation in utilizing this rapid detection for tiger shrimp PL exposed beyond 12 hours of V. harveyi.
Keywords: specific primer, luminous Vibrio bacteria, pathogenic, PCR method, hemolysin gene
ABSTRAK
Penelitian ini bertujuan untuk mengetahui kemampuan atau sensitivitas primer hemolisin dalam mendeteksi Vibrio patogen dengan lama pemaparan berbeda. Penelitian ini dilakukan dengan menginfeksikan Vibrio harveyi pada benur udang dengan metode perendaman pada konsentrasi 106 cfu/mL. Pengambilan sampel dilakukan pada waktu tiga, enam, 12, 24, 48, dan 72 jam pascainfeksi. Keberadaan gen hemolisin pada bakteri V. harveyi dideteksi menggunakan teknik polymerase chain reaction (PCR). Hasil elektroforesis memperlihatkan bahwa pada pemaparan tiga dan enam jam keberadaan gen hemolisin dari bakteri Vibrio patogen yang diinfeksikan sudah dapat terdeteksi pada benur walaupun masih terlihat tipis. Pada pemaparan 12 jam terlihat sangat jelas pita-pita DNA dari bakteri patogen. Sedangkan pada pemaparan 24, 48, dan 72 jam sudah tidak terdeteksi lagi gen hemolisin dari bakteri Vibrio. Hal ini diduga disebabkan terjadinya penurunan populasi bakteri Vibrio yang hidup dalam tubuh dan media pemeliharaan udang. Pentingnya deteksi cepat diawal udang terinfeksi bakteri (3–12 jam) karena setelah 12 jam infeksi sudah sulit untuk mendeteksi keberadaan bakteri patogen di dalam tubuh udang.
Kata kunci: primer spesifik, bakteri Vibrio berpendar, patogenik, metode PCR, gen hemolisin
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| format | Article |
| id | doaj-art-5c1711b04483450ab798faa2bc2ec971 |
| institution | Kabale University |
| issn | 1412-5269 2354-6700 |
| language | English |
| publishDate | 2015-05-01 |
| publisher | Asosiasi Sains Akuakultur Indonesia |
| record_format | Article |
| series | Jurnal Akuakultur Indonesia |
| spelling | doaj-art-5c1711b04483450ab798faa2bc2ec9712025-01-24T17:59:25ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002015-05-0112210.19027/jai.12.101-1057827Detection of Vibrio harveyi using hemolysin primer in tiger shrimp Penaeus monodonIrma Suriyani0Ince Ayu Khairana Kadriah1ilmiah Kuruseng2Jurusan Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Muslim Indonesia Jl. Urip Sumiharjo. KM. 05 Makassar 90231Balai Penelitian Pengembangan Budidaya Air Payau (BPPBAP) Jl. Makmur Dg. Sitakka No. 129 Maros. 90512Jurusan Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Muslim Indonesia Jl. Urip Sumiharjo. KM. 05 Makassar 90231ABSTRACT This study was aimed to analyze the sensitivity and ability of primer hemolysin in detecting pathogenetic Vibrio on tiger shrimp post-larvae (PL) exposed under different exposure times in media inoculated with Vibrio harveyi. The PL of tiger shrimp were infected with 106 cfu/mL of V. harveyi by immersion method for three, six, 12, 24, 48 and 72 hours. The presence of hemolisin genes was detected by PCR techniques. The electrophoresis detected narrow hemolysin genes after PL were exposed for three and six hours. Clear visible bands of DNA Vibrio were observed for 12 hours of exposure. In contrast, no detected hemolysin gene of Vibrio was observed for PL exposed within 24, 48, and 72 hours. The rapid detection on Vibrio pathogenic for tiger shrimp PL should be conducted within three to 12 hours of exposure. No recommendation in utilizing this rapid detection for tiger shrimp PL exposed beyond 12 hours of V. harveyi. Keywords: specific primer, luminous Vibrio bacteria, pathogenic, PCR method, hemolysin gene ABSTRAK Penelitian ini bertujuan untuk mengetahui kemampuan atau sensitivitas primer hemolisin dalam mendeteksi Vibrio patogen dengan lama pemaparan berbeda. Penelitian ini dilakukan dengan menginfeksikan Vibrio harveyi pada benur udang dengan metode perendaman pada konsentrasi 106 cfu/mL. Pengambilan sampel dilakukan pada waktu tiga, enam, 12, 24, 48, dan 72 jam pascainfeksi. Keberadaan gen hemolisin pada bakteri V. harveyi dideteksi menggunakan teknik polymerase chain reaction (PCR). Hasil elektroforesis memperlihatkan bahwa pada pemaparan tiga dan enam jam keberadaan gen hemolisin dari bakteri Vibrio patogen yang diinfeksikan sudah dapat terdeteksi pada benur walaupun masih terlihat tipis. Pada pemaparan 12 jam terlihat sangat jelas pita-pita DNA dari bakteri patogen. Sedangkan pada pemaparan 24, 48, dan 72 jam sudah tidak terdeteksi lagi gen hemolisin dari bakteri Vibrio. Hal ini diduga disebabkan terjadinya penurunan populasi bakteri Vibrio yang hidup dalam tubuh dan media pemeliharaan udang. Pentingnya deteksi cepat diawal udang terinfeksi bakteri (3–12 jam) karena setelah 12 jam infeksi sudah sulit untuk mendeteksi keberadaan bakteri patogen di dalam tubuh udang. Kata kunci: primer spesifik, bakteri Vibrio berpendar, patogenik, metode PCR, gen hemolisin https://journal.ipb.ac.id/index.php/jai/article/view/9362 |
| spellingShingle | Irma Suriyani Ince Ayu Khairana Kadriah ilmiah Kuruseng Detection of Vibrio harveyi using hemolysin primer in tiger shrimp Penaeus monodon Jurnal Akuakultur Indonesia |
| title | Detection of Vibrio harveyi using hemolysin primer in tiger shrimp Penaeus monodon |
| title_full | Detection of Vibrio harveyi using hemolysin primer in tiger shrimp Penaeus monodon |
| title_fullStr | Detection of Vibrio harveyi using hemolysin primer in tiger shrimp Penaeus monodon |
| title_full_unstemmed | Detection of Vibrio harveyi using hemolysin primer in tiger shrimp Penaeus monodon |
| title_short | Detection of Vibrio harveyi using hemolysin primer in tiger shrimp Penaeus monodon |
| title_sort | detection of vibrio harveyi using hemolysin primer in tiger shrimp penaeus monodon |
| url | https://journal.ipb.ac.id/index.php/jai/article/view/9362 |
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