Proinflammatory Effects of Diesel Exhaust Nanoparticles on Scleroderma Skin Cells
Autoimmune diseases are complex disorders of unknown etiology thought to result from interactions between genetic and environmental factors. We aimed to verify whether environmental pollution from diesel engine exhaust nanoparticulate (DEP) of actually operating vehicles could play a role in the dev...
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Wiley
2014-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2014/138751 |
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| author | A. Mastrofrancesco M. Alfè E. Rosato V. Gargiulo C. Beatrice G. Di Blasio B. Zhang D. S. Su M. Picardo S. Fiorito |
| author_facet | A. Mastrofrancesco M. Alfè E. Rosato V. Gargiulo C. Beatrice G. Di Blasio B. Zhang D. S. Su M. Picardo S. Fiorito |
| author_sort | A. Mastrofrancesco |
| collection | DOAJ |
| description | Autoimmune diseases are complex disorders of unknown etiology thought to result from interactions between genetic and environmental factors. We aimed to verify whether environmental pollution from diesel engine exhaust nanoparticulate (DEP) of actually operating vehicles could play a role in the development of a rare immune-mediated disease, systemic sclerosis (SSc), in which the pathogenetic role of environment has been highlighted. The effects of carbon-based nanoparticulate collected at the exhaust of newer (Euro 5) and older (Euro 4) diesel engines on SSc skin keratinocytes and fibroblasts were evaluated in vitro by assessing the mRNA expression of inflammatory cytokines (IL-1α, IL-6, IL-8, and TNF-α) and fibroblast chemical mediators (metalloproteases 2, 3, 7, 9, and 12; collagen types I and III; VEGF). DEP was shown to stimulate cytokine gene expression at a higher extent in SSc keratinocytes versus normal cells. Moreover, the mRNA gene expression of all MMPs, collagen types, and VEGF genes was significantly higher in untreated SSc fibroblasts versus controls. Euro 5 particle exposure increased the mRNA expression of MMP-2, -7, and -9 in SSc fibroblasts in a dose dependent manner and only at the highest concentration in normal cells. We suggest that environmental DEP could trigger the development of SSc acting on genetically hyperreactive cell systems. |
| format | Article |
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| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-5c1528056a8744e7941c45b49eb529c42025-02-03T06:14:13ZengWileyJournal of Immunology Research2314-88612314-71562014-01-01201410.1155/2014/138751138751Proinflammatory Effects of Diesel Exhaust Nanoparticles on Scleroderma Skin CellsA. Mastrofrancesco0M. Alfè1E. Rosato2V. Gargiulo3C. Beatrice4G. Di Blasio5B. Zhang6D. S. Su7M. Picardo8S. Fiorito9Laboratorio di Fisiopatologia Cutanea, Istituto Dermatologico S Gallicano, Via E. Chianesi 53, 00144 Roma, ItalyIstituto di Ricerche sulla Combustione (IRC), CNR, Piazzale V. Tecchio 80, 80125 Napoli, ItalyDipartimento di Medicina Clinica, Università Sapienza, CNR, Viale dell'Università 37, 00185 Roma, ItalyIstituto di Ricerche sulla Combustione (IRC), CNR, Piazzale V. Tecchio 80, 80125 Napoli, ItalyIstituto Motori (IM), Via Marconi 8, 80125 Napoli, ItalyIstituto Motori (IM), Via Marconi 8, 80125 Napoli, ItalyShenyang National Laboratory for Materials Science, Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, ChinaShenyang National Laboratory for Materials Science, Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, ChinaLaboratorio di Fisiopatologia Cutanea, Istituto Dermatologico S Gallicano, Via E. Chianesi 53, 00144 Roma, ItalyDipartimento di Medicina Clinica, Università Sapienza, CNR, Viale dell'Università 37, 00185 Roma, ItalyAutoimmune diseases are complex disorders of unknown etiology thought to result from interactions between genetic and environmental factors. We aimed to verify whether environmental pollution from diesel engine exhaust nanoparticulate (DEP) of actually operating vehicles could play a role in the development of a rare immune-mediated disease, systemic sclerosis (SSc), in which the pathogenetic role of environment has been highlighted. The effects of carbon-based nanoparticulate collected at the exhaust of newer (Euro 5) and older (Euro 4) diesel engines on SSc skin keratinocytes and fibroblasts were evaluated in vitro by assessing the mRNA expression of inflammatory cytokines (IL-1α, IL-6, IL-8, and TNF-α) and fibroblast chemical mediators (metalloproteases 2, 3, 7, 9, and 12; collagen types I and III; VEGF). DEP was shown to stimulate cytokine gene expression at a higher extent in SSc keratinocytes versus normal cells. Moreover, the mRNA gene expression of all MMPs, collagen types, and VEGF genes was significantly higher in untreated SSc fibroblasts versus controls. Euro 5 particle exposure increased the mRNA expression of MMP-2, -7, and -9 in SSc fibroblasts in a dose dependent manner and only at the highest concentration in normal cells. We suggest that environmental DEP could trigger the development of SSc acting on genetically hyperreactive cell systems.http://dx.doi.org/10.1155/2014/138751 |
| spellingShingle | A. Mastrofrancesco M. Alfè E. Rosato V. Gargiulo C. Beatrice G. Di Blasio B. Zhang D. S. Su M. Picardo S. Fiorito Proinflammatory Effects of Diesel Exhaust Nanoparticles on Scleroderma Skin Cells Journal of Immunology Research |
| title | Proinflammatory Effects of Diesel Exhaust Nanoparticles on Scleroderma Skin Cells |
| title_full | Proinflammatory Effects of Diesel Exhaust Nanoparticles on Scleroderma Skin Cells |
| title_fullStr | Proinflammatory Effects of Diesel Exhaust Nanoparticles on Scleroderma Skin Cells |
| title_full_unstemmed | Proinflammatory Effects of Diesel Exhaust Nanoparticles on Scleroderma Skin Cells |
| title_short | Proinflammatory Effects of Diesel Exhaust Nanoparticles on Scleroderma Skin Cells |
| title_sort | proinflammatory effects of diesel exhaust nanoparticles on scleroderma skin cells |
| url | http://dx.doi.org/10.1155/2014/138751 |
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