Puerarin Suppress Apoptosis of Human Osteoblasts via ERK Signaling Pathway
Puerarin, the main isoflavone glycoside extracted from Radix Puerariae, is an isoflavone traditional Chinese herb. Previous studies have demonstrated that puerarin could regulate osteoblast proliferation and differentiation to promote bone formation. However, the effect of puerarin on the process of...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2013/786574 |
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| author | Ling-juan Liu Li-qun Liu Tao Bo Shi-jun Li Zhen Zhu Rong-rong Cui Ding-an Mao |
| author_facet | Ling-juan Liu Li-qun Liu Tao Bo Shi-jun Li Zhen Zhu Rong-rong Cui Ding-an Mao |
| author_sort | Ling-juan Liu |
| collection | DOAJ |
| description | Puerarin, the main isoflavone glycoside extracted from Radix Puerariae, is an isoflavone traditional Chinese herb. Previous studies have demonstrated that puerarin could regulate osteoblast proliferation and differentiation to promote bone formation. However, the effect of puerarin on the process of human osteoblasts (hOBs) apoptosis is still unclear. In this study, we detected the function of puerarin on serum-free-induced cell apoptosis using ELISA and TUNEL arrays and then found that the mortality of hOBs was significantly decreased after exposure to 10−10–10−6 M puerarin and reached the maximal antiapoptotic effect at the concentration of 10−8 M. In addition, compared with the control group, puerarin notably increased the Bcl-2 protein levels while it decreased the Bax protein levels in the hOBs in a dose-dependent way. 10−7 M puerarin decreased the Bax/Bcl-2 ratio with a maximal decrease to 0.08. Moreover, puerarin activated ERK signaling pathways in hOBs, and the antiapoptotic effect induced by puerarin was abolished by incubation of ERK inhibitor PD98059. Similarly, the estrogen receptor antagonist ICI182780 also suppressed the inhibitory effect of puerarin on hOBs apoptosis. In conclusion, puerarin could prevent hOBs apoptosis via ERK signaling pathway, which might be effective in providing protection against bone loss and bone remolding associated with osteoporosis. |
| format | Article |
| id | doaj-art-5bdf6b8aa5544719b8bcfd5496dc7130 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-5bdf6b8aa5544719b8bcfd5496dc71302025-02-03T01:33:01ZengWileyInternational Journal of Endocrinology1687-83371687-83452013-01-01201310.1155/2013/786574786574Puerarin Suppress Apoptosis of Human Osteoblasts via ERK Signaling PathwayLing-juan Liu0Li-qun Liu1Tao Bo2Shi-jun Li3Zhen Zhu4Rong-rong Cui5Ding-an Mao6Department of Pediatrics, The Second Xiang-Ya Hospital, Central South University, 139 Middle Renmin Road, Changsha, Hunan 410011, ChinaDepartment of Pediatrics, The Second Xiang-Ya Hospital, Central South University, 139 Middle Renmin Road, Changsha, Hunan 410011, ChinaDepartment of Pediatrics, The Second Xiang-Ya Hospital, Central South University, 139 Middle Renmin Road, Changsha, Hunan 410011, ChinaDepartment of Pediatrics, The Second Xiang-Ya Hospital, Central South University, 139 Middle Renmin Road, Changsha, Hunan 410011, ChinaDepartment of Pediatrics, The Second Xiang-Ya Hospital, Central South University, 139 Middle Renmin Road, Changsha, Hunan 410011, ChinaInstitute of Metabolism and Endocrinology, The Second Xiang-Ya Hospital, Central South University, 139 Middle Renmin Road, Changsha, Hunan 410011, ChinaDepartment of Pediatrics, The Second Xiang-Ya Hospital, Central South University, 139 Middle Renmin Road, Changsha, Hunan 410011, ChinaPuerarin, the main isoflavone glycoside extracted from Radix Puerariae, is an isoflavone traditional Chinese herb. Previous studies have demonstrated that puerarin could regulate osteoblast proliferation and differentiation to promote bone formation. However, the effect of puerarin on the process of human osteoblasts (hOBs) apoptosis is still unclear. In this study, we detected the function of puerarin on serum-free-induced cell apoptosis using ELISA and TUNEL arrays and then found that the mortality of hOBs was significantly decreased after exposure to 10−10–10−6 M puerarin and reached the maximal antiapoptotic effect at the concentration of 10−8 M. In addition, compared with the control group, puerarin notably increased the Bcl-2 protein levels while it decreased the Bax protein levels in the hOBs in a dose-dependent way. 10−7 M puerarin decreased the Bax/Bcl-2 ratio with a maximal decrease to 0.08. Moreover, puerarin activated ERK signaling pathways in hOBs, and the antiapoptotic effect induced by puerarin was abolished by incubation of ERK inhibitor PD98059. Similarly, the estrogen receptor antagonist ICI182780 also suppressed the inhibitory effect of puerarin on hOBs apoptosis. In conclusion, puerarin could prevent hOBs apoptosis via ERK signaling pathway, which might be effective in providing protection against bone loss and bone remolding associated with osteoporosis.http://dx.doi.org/10.1155/2013/786574 |
| spellingShingle | Ling-juan Liu Li-qun Liu Tao Bo Shi-jun Li Zhen Zhu Rong-rong Cui Ding-an Mao Puerarin Suppress Apoptosis of Human Osteoblasts via ERK Signaling Pathway International Journal of Endocrinology |
| title | Puerarin Suppress Apoptosis of Human Osteoblasts via ERK Signaling Pathway |
| title_full | Puerarin Suppress Apoptosis of Human Osteoblasts via ERK Signaling Pathway |
| title_fullStr | Puerarin Suppress Apoptosis of Human Osteoblasts via ERK Signaling Pathway |
| title_full_unstemmed | Puerarin Suppress Apoptosis of Human Osteoblasts via ERK Signaling Pathway |
| title_short | Puerarin Suppress Apoptosis of Human Osteoblasts via ERK Signaling Pathway |
| title_sort | puerarin suppress apoptosis of human osteoblasts via erk signaling pathway |
| url | http://dx.doi.org/10.1155/2013/786574 |
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