A simple method to generate stable cell lines for the analysis of transient protein-protein interactions

A simple method to generate stable cell lines for the analysis of transient protein-protein interactions

Transient protein-protein interactions form the basis of signal transduction pathways in addition to many other biological processes. One tool for studying these interactions is bioluminescence resonance energ y transfer (BRET). This technique has been widely applied to study signaling pathways, in...

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Bibliographic Details
Main Authors: Emilia Elizabeth Savage, Denise Wootten, Arthur Christopoulos, Patrick Michael Sexton, Sebastian George Barton Furness
Format: Article
Language:English
Published: Taylor & Francis Group 2013-04-01
Series:BioTechniques
Subjects:
BRET
flpIN
Gateway
protein interaction
bicistronic vector
arrestin
Online Access:https://www.future-science.com/doi/10.2144/000114013
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https://www.future-science.com/doi/10.2144/000114013

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