Identifying RBBP7 as a Promising Diagnostic Biomarker for BK Virus-Associated Nephropathy
BK virus-associated nephropathy (BKVN) remains a major infectious complication due to powerful immunosuppression in kidney transplant recipients, and its histologic appearance can mimic rejection, leading to diagnostic and treatment dilemmas thus molecular diagnostic methods would be beneficial. We...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/6934744 |
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| author | Yicun Wang Yuxuan Wang Di Zhang Hao Zhang Wei Wang Xiaopeng Hu |
| author_facet | Yicun Wang Yuxuan Wang Di Zhang Hao Zhang Wei Wang Xiaopeng Hu |
| author_sort | Yicun Wang |
| collection | DOAJ |
| description | BK virus-associated nephropathy (BKVN) remains a major infectious complication due to powerful immunosuppression in kidney transplant recipients, and its histologic appearance can mimic rejection, leading to diagnostic and treatment dilemmas thus molecular diagnostic methods would be beneficial. We collected gene expression profiles of 169 kidney biopsies taken from BKVN, rejection, and stable functioning allografts, based on single sample gene set enrichment analysis and random forest algorithm, and three hallmark activities associated with DNA damage and proliferation were found to be relatively specific in BKVN. Subsequently, weighted gene co-expression network analysis and support vector machines (SVM) algorithm identified RBBP7 as a robust and promising biomarker with high accuracy in both training and validation cohorts (AUC =0.938, 0.977, respectively). Besides, potential drugs for BKVN treatment such as mepacrine were discovered, which may contribute to targeted antiviral therapy and effective patient management rather than simply reducing the doses of immunosuppressive agents in clinical practice. RBBP7 (retinoblastoma binding protein 7) serves as component of serval complexes that regulate chromatin metabolism and functions in affecting DNA replication and controlling cell proliferation. In this research, upregulation of RBBP7 was found to be associated with the higher infiltration of CD8 naïve T, iTreg, and neutrophil cells and the lower amounts of Th1, central memory T, NKT, CD8 T, and dendritic cells. Moreover, the infiltration of Th1, Th17, and NKT cells was steadily different between BKVN and rejection allografts through immune cell assessment. In conclusion, we identified and verified RBBP7 as a molecular biomarker for BKVN diagnosis, which demonstrated great distinguishing ability with allograft rejection and would support clinical decision-making. |
| format | Article |
| id | doaj-art-5bad36df1fdb40828ec709a0e5eee41c |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-5bad36df1fdb40828ec709a0e5eee41c2025-02-03T05:50:44ZengWileyJournal of Immunology Research2314-71562022-01-01202210.1155/2022/6934744Identifying RBBP7 as a Promising Diagnostic Biomarker for BK Virus-Associated NephropathyYicun Wang0Yuxuan Wang1Di Zhang2Hao Zhang3Wei Wang4Xiaopeng Hu5Department of UrologyDepartment of UrologyDepartment of UrologyDepartment of UrologyDepartment of UrologyDepartment of UrologyBK virus-associated nephropathy (BKVN) remains a major infectious complication due to powerful immunosuppression in kidney transplant recipients, and its histologic appearance can mimic rejection, leading to diagnostic and treatment dilemmas thus molecular diagnostic methods would be beneficial. We collected gene expression profiles of 169 kidney biopsies taken from BKVN, rejection, and stable functioning allografts, based on single sample gene set enrichment analysis and random forest algorithm, and three hallmark activities associated with DNA damage and proliferation were found to be relatively specific in BKVN. Subsequently, weighted gene co-expression network analysis and support vector machines (SVM) algorithm identified RBBP7 as a robust and promising biomarker with high accuracy in both training and validation cohorts (AUC =0.938, 0.977, respectively). Besides, potential drugs for BKVN treatment such as mepacrine were discovered, which may contribute to targeted antiviral therapy and effective patient management rather than simply reducing the doses of immunosuppressive agents in clinical practice. RBBP7 (retinoblastoma binding protein 7) serves as component of serval complexes that regulate chromatin metabolism and functions in affecting DNA replication and controlling cell proliferation. In this research, upregulation of RBBP7 was found to be associated with the higher infiltration of CD8 naïve T, iTreg, and neutrophil cells and the lower amounts of Th1, central memory T, NKT, CD8 T, and dendritic cells. Moreover, the infiltration of Th1, Th17, and NKT cells was steadily different between BKVN and rejection allografts through immune cell assessment. In conclusion, we identified and verified RBBP7 as a molecular biomarker for BKVN diagnosis, which demonstrated great distinguishing ability with allograft rejection and would support clinical decision-making.http://dx.doi.org/10.1155/2022/6934744 |
| spellingShingle | Yicun Wang Yuxuan Wang Di Zhang Hao Zhang Wei Wang Xiaopeng Hu Identifying RBBP7 as a Promising Diagnostic Biomarker for BK Virus-Associated Nephropathy Journal of Immunology Research |
| title | Identifying RBBP7 as a Promising Diagnostic Biomarker for BK Virus-Associated Nephropathy |
| title_full | Identifying RBBP7 as a Promising Diagnostic Biomarker for BK Virus-Associated Nephropathy |
| title_fullStr | Identifying RBBP7 as a Promising Diagnostic Biomarker for BK Virus-Associated Nephropathy |
| title_full_unstemmed | Identifying RBBP7 as a Promising Diagnostic Biomarker for BK Virus-Associated Nephropathy |
| title_short | Identifying RBBP7 as a Promising Diagnostic Biomarker for BK Virus-Associated Nephropathy |
| title_sort | identifying rbbp7 as a promising diagnostic biomarker for bk virus associated nephropathy |
| url | http://dx.doi.org/10.1155/2022/6934744 |
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