Haemaphysalis longicornis subolesin controls the infection and transmission of severe fever with thrombocytopenia syndrome virus
Abstract Severe fever with thrombocytopenia syndrome (SFTS) caused by the SFTS virus (SFTSV) is an emerging tick-borne disease with a high mortality rate. Haemaphysalis longicornis is the primary reservoir and vector of SFTSV. Here, we found that targeting subolesin (SUB), an anti-tick vaccine candi...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | npj Vaccines |
| Online Access: | https://doi.org/10.1038/s41541-024-01061-1 |
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| author | Chuanfei Yuan Yu Sun Jingjing Chen Qiong Xu Xiang Zhou Zhen Zou Qianfeng Xia |
| author_facet | Chuanfei Yuan Yu Sun Jingjing Chen Qiong Xu Xiang Zhou Zhen Zou Qianfeng Xia |
| author_sort | Chuanfei Yuan |
| collection | DOAJ |
| description | Abstract Severe fever with thrombocytopenia syndrome (SFTS) caused by the SFTS virus (SFTSV) is an emerging tick-borne disease with a high mortality rate. Haemaphysalis longicornis is the primary reservoir and vector of SFTSV. Here, we found that targeting subolesin (SUB), an anti-tick vaccine candidate, affects the infection and transmission of SFTSV in H. longicornis. RNAi-mediated knockdown of SUB repressed SFTSV infection in the salivary glands but not in the gut of H. longicornis, which may be associated with the modulation of protein processing in endoplasmic reticulum revealed by transcriptomic analysis. Knockdown of SUB decreased the survival and engorgement rates of ticks and impaired the horizontal and co-feeding transmission of SFTSV. Furthermore, active immunization with recombinant SUB inhibited the co-feeding transmission of SFTSV, although it had no significant effect on the blood-feeding behavior of infected ticks. Collectively, these results provide a potential target for controlling SFTS and other tick-borne viral diseases. |
| format | Article |
| id | doaj-art-5b8333609dd6439aa2ed7f1b294eefd0 |
| institution | Kabale University |
| issn | 2059-0105 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Vaccines |
| spelling | doaj-art-5b8333609dd6439aa2ed7f1b294eefd02025-01-26T12:14:11ZengNature Portfolionpj Vaccines2059-01052025-01-0110111010.1038/s41541-024-01061-1Haemaphysalis longicornis subolesin controls the infection and transmission of severe fever with thrombocytopenia syndrome virusChuanfei Yuan0Yu Sun1Jingjing Chen2Qiong Xu3Xiang Zhou4Zhen Zou5Qianfeng Xia6NHC Key Laboratory of Tropical Disease Control, School of Tropical Medicine, Hainan Medical UniversityNHC Key Laboratory of Tropical Disease Control, School of Tropical Medicine, Hainan Medical UniversityNHC Key Laboratory of Tropical Disease Control, School of Tropical Medicine, Hainan Medical UniversityNHC Key Laboratory of Tropical Disease Control, School of Tropical Medicine, Hainan Medical UniversityNHC Key Laboratory of Tropical Disease Control, School of Tropical Medicine, Hainan Medical UniversityState Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of SciencesNHC Key Laboratory of Tropical Disease Control, School of Tropical Medicine, Hainan Medical UniversityAbstract Severe fever with thrombocytopenia syndrome (SFTS) caused by the SFTS virus (SFTSV) is an emerging tick-borne disease with a high mortality rate. Haemaphysalis longicornis is the primary reservoir and vector of SFTSV. Here, we found that targeting subolesin (SUB), an anti-tick vaccine candidate, affects the infection and transmission of SFTSV in H. longicornis. RNAi-mediated knockdown of SUB repressed SFTSV infection in the salivary glands but not in the gut of H. longicornis, which may be associated with the modulation of protein processing in endoplasmic reticulum revealed by transcriptomic analysis. Knockdown of SUB decreased the survival and engorgement rates of ticks and impaired the horizontal and co-feeding transmission of SFTSV. Furthermore, active immunization with recombinant SUB inhibited the co-feeding transmission of SFTSV, although it had no significant effect on the blood-feeding behavior of infected ticks. Collectively, these results provide a potential target for controlling SFTS and other tick-borne viral diseases.https://doi.org/10.1038/s41541-024-01061-1 |
| spellingShingle | Chuanfei Yuan Yu Sun Jingjing Chen Qiong Xu Xiang Zhou Zhen Zou Qianfeng Xia Haemaphysalis longicornis subolesin controls the infection and transmission of severe fever with thrombocytopenia syndrome virus npj Vaccines |
| title | Haemaphysalis longicornis subolesin controls the infection and transmission of severe fever with thrombocytopenia syndrome virus |
| title_full | Haemaphysalis longicornis subolesin controls the infection and transmission of severe fever with thrombocytopenia syndrome virus |
| title_fullStr | Haemaphysalis longicornis subolesin controls the infection and transmission of severe fever with thrombocytopenia syndrome virus |
| title_full_unstemmed | Haemaphysalis longicornis subolesin controls the infection and transmission of severe fever with thrombocytopenia syndrome virus |
| title_short | Haemaphysalis longicornis subolesin controls the infection and transmission of severe fever with thrombocytopenia syndrome virus |
| title_sort | haemaphysalis longicornis subolesin controls the infection and transmission of severe fever with thrombocytopenia syndrome virus |
| url | https://doi.org/10.1038/s41541-024-01061-1 |
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