Presence of dengue virus RNA in urine and oral fluid of laboratory-confirmed dengue patients: Implications for wastewater surveillance
Introduction: Dengue cases in the Americas in 2024 have reached record highs, especially in Brazil. However, surveillance remains suboptimal and new methods are needed to monitor Dengue Virus (DENV) spread. To assess whether wastewater-based epidemiology would be a useful tool, we investigated the p...
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Elsevier
2025-01-01
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| Series: | Brazilian Journal of Infectious Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1413867024007670 |
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| author | Christine Stauber Leile Camila Jacob-Nascimento Caroline Grosch Moisés da Silva Sousa Moyra M. Portilho Rosângela O. Anjos Margo A. Brinton Uriel Kitron Mitermayer G. Reis Guilherme S. Ribeiro |
| author_facet | Christine Stauber Leile Camila Jacob-Nascimento Caroline Grosch Moisés da Silva Sousa Moyra M. Portilho Rosângela O. Anjos Margo A. Brinton Uriel Kitron Mitermayer G. Reis Guilherme S. Ribeiro |
| author_sort | Christine Stauber |
| collection | DOAJ |
| description | Introduction: Dengue cases in the Americas in 2024 have reached record highs, especially in Brazil. However, surveillance remains suboptimal and new methods are needed to monitor Dengue Virus (DENV) spread. To assess whether wastewater-based epidemiology would be a useful tool, we investigated the presence of DENV RNA in dengue patients’ urine and oral fluid from an endemic area to inform how shedding in these fluids occurs and provide insight for wastewater surveillance. Methods: We examined how often DENV RNA is detected in urine and oral fluid from dengue patients confirmed by serum RT-qPCR, NS1 ELISA or IgM seroconversion in Salvador, Brazil. Results: Of 88 confirmed cases, 9.1 % were positive for DENV RNA in urine (7/88) or oral fluid (1/88). Of 53 serum RT-qPCR-positive patients, 6 (11.3 %) showed detectable DENV RNA in acute- or convalescent-phase urine. Patients with RT-qPCR-positive urine had a lower frequency of DENV IgG in acute-phase serum (a proxy for secondary infection) (57 % vs. 74 %) and a lower median serum RT-qPCR cycle threshold than those with negative urine (21.8 vs. 23.9). Conclusion: The low presence of DENV RNA in urine suggests that additional research is needed to evaluate whether using wastewater-based epidemiology to monitor DENV transmission is possible. |
| format | Article |
| id | doaj-art-5b73bcdcea274d53a282d50808b3a449 |
| institution | Kabale University |
| issn | 1413-8670 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj-art-5b73bcdcea274d53a282d50808b3a4492025-01-26T05:03:34ZengElsevierBrazilian Journal of Infectious Diseases1413-86702025-01-01291104484Presence of dengue virus RNA in urine and oral fluid of laboratory-confirmed dengue patients: Implications for wastewater surveillanceChristine Stauber0Leile Camila Jacob-Nascimento1Caroline Grosch2Moisés da Silva Sousa3Moyra M. Portilho4Rosângela O. Anjos5Margo A. Brinton6Uriel Kitron7Mitermayer G. Reis8Guilherme S. Ribeiro9Georgia State University, School of Public Health, Atlanta, USAFundação Oswaldo Cruz, Instituto Gonçalo Moniz, Salvador, BA, Brazil; Universidade Federal da Bahia, Faculdade de Medicina da Bahia, Salvador, BA, BrazilGeorgia State University, School of Public Health, Atlanta, USAFundação Oswaldo Cruz, Instituto Gonçalo Moniz, Salvador, BA, BrazilFundação Oswaldo Cruz, Instituto Gonçalo Moniz, Salvador, BA, BrazilFundação Oswaldo Cruz, Instituto Gonçalo Moniz, Salvador, BA, BrazilGeorgia State University, College of Arts and Sciences, Department of Biology, Atlanta, USAEmory University, Department of Environmental Sciences, Atlanta, USAFundação Oswaldo Cruz, Instituto Gonçalo Moniz, Salvador, BA, Brazil; Universidade Federal da Bahia, Faculdade de Medicina da Bahia, Salvador, BA, Brazil; Yale University, Yale School of Public Health, New Haven, USAFundação Oswaldo Cruz, Instituto Gonçalo Moniz, Salvador, BA, Brazil; Universidade Federal da Bahia, Faculdade de Medicina da Bahia, Salvador, BA, Brazil; Corresponding author.Introduction: Dengue cases in the Americas in 2024 have reached record highs, especially in Brazil. However, surveillance remains suboptimal and new methods are needed to monitor Dengue Virus (DENV) spread. To assess whether wastewater-based epidemiology would be a useful tool, we investigated the presence of DENV RNA in dengue patients’ urine and oral fluid from an endemic area to inform how shedding in these fluids occurs and provide insight for wastewater surveillance. Methods: We examined how often DENV RNA is detected in urine and oral fluid from dengue patients confirmed by serum RT-qPCR, NS1 ELISA or IgM seroconversion in Salvador, Brazil. Results: Of 88 confirmed cases, 9.1 % were positive for DENV RNA in urine (7/88) or oral fluid (1/88). Of 53 serum RT-qPCR-positive patients, 6 (11.3 %) showed detectable DENV RNA in acute- or convalescent-phase urine. Patients with RT-qPCR-positive urine had a lower frequency of DENV IgG in acute-phase serum (a proxy for secondary infection) (57 % vs. 74 %) and a lower median serum RT-qPCR cycle threshold than those with negative urine (21.8 vs. 23.9). Conclusion: The low presence of DENV RNA in urine suggests that additional research is needed to evaluate whether using wastewater-based epidemiology to monitor DENV transmission is possible.http://www.sciencedirect.com/science/article/pii/S1413867024007670EpidemiologyDengue virusWastewater-based epidemiology |
| spellingShingle | Christine Stauber Leile Camila Jacob-Nascimento Caroline Grosch Moisés da Silva Sousa Moyra M. Portilho Rosângela O. Anjos Margo A. Brinton Uriel Kitron Mitermayer G. Reis Guilherme S. Ribeiro Presence of dengue virus RNA in urine and oral fluid of laboratory-confirmed dengue patients: Implications for wastewater surveillance Brazilian Journal of Infectious Diseases Epidemiology Dengue virus Wastewater-based epidemiology |
| title | Presence of dengue virus RNA in urine and oral fluid of laboratory-confirmed dengue patients: Implications for wastewater surveillance |
| title_full | Presence of dengue virus RNA in urine and oral fluid of laboratory-confirmed dengue patients: Implications for wastewater surveillance |
| title_fullStr | Presence of dengue virus RNA in urine and oral fluid of laboratory-confirmed dengue patients: Implications for wastewater surveillance |
| title_full_unstemmed | Presence of dengue virus RNA in urine and oral fluid of laboratory-confirmed dengue patients: Implications for wastewater surveillance |
| title_short | Presence of dengue virus RNA in urine and oral fluid of laboratory-confirmed dengue patients: Implications for wastewater surveillance |
| title_sort | presence of dengue virus rna in urine and oral fluid of laboratory confirmed dengue patients implications for wastewater surveillance |
| topic | Epidemiology Dengue virus Wastewater-based epidemiology |
| url | http://www.sciencedirect.com/science/article/pii/S1413867024007670 |
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