Regulation of Ghrelin Receptor by Periodontal Bacteria In Vitro and In Vivo
Ghrelin plays a major role in obesity-related diseases which have been shown to be associated with periodontitis. This study sought to analyze the expression of the functional receptor for ghrelin (GHS-R1a) in periodontal cells and tissues under microbial conditions in vitro and in vivo. The GHS-R1a...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/4916971 |
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| author | Marjan Nokhbehsaim Anna Damanaki Andressa Vilas Boas Nogueira Sigrun Eick Svenja Memmert Xiaoyan Zhou Shanika Nanayakkara Werner Götz Joni Augusto Cirelli Andreas Jäger James Deschner |
| author_facet | Marjan Nokhbehsaim Anna Damanaki Andressa Vilas Boas Nogueira Sigrun Eick Svenja Memmert Xiaoyan Zhou Shanika Nanayakkara Werner Götz Joni Augusto Cirelli Andreas Jäger James Deschner |
| author_sort | Marjan Nokhbehsaim |
| collection | DOAJ |
| description | Ghrelin plays a major role in obesity-related diseases which have been shown to be associated with periodontitis. This study sought to analyze the expression of the functional receptor for ghrelin (GHS-R1a) in periodontal cells and tissues under microbial conditions in vitro and in vivo. The GHS-R1a expression in human periodontal cells challenged with the periodontopathogen Fusobacterium nucleatum, in gingival biopsies from periodontally healthy and diseased individuals, and from rats with and without ligature-induced periodontitis was analyzed by real-time PCR, immunocytochemistry, and immunofluorescence. F. nucleatum induced an initial upregulation and subsequent downregulation of GHS-R1a in periodontal cells. In rat experimental periodontitis, the GHS-R1a expression at periodontitis sites was increased during the early stage of periodontitis, but significantly reduced afterwards, when compared with healthy sites. In human gingival biopsies, periodontally diseased sites showed a significantly lower GHS-R1a expression than the healthy sites. The expression of the functional ghrelin receptor in periodontal cells and tissues is modulated by periodontal bacteria. Due to the downregulation of the functional ghrelin receptor by long-term exposure to periodontal bacteria, the anti-inflammatory actions of ghrelin may be diminished in chronic periodontal infections, which could lead to an enhanced periodontal inflammation and tissue destruction. |
| format | Article |
| id | doaj-art-5b5c36b6e9c54fdc992d844854756153 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-5b5c36b6e9c54fdc992d8448547561532025-02-03T06:12:45ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/49169714916971Regulation of Ghrelin Receptor by Periodontal Bacteria In Vitro and In VivoMarjan Nokhbehsaim0Anna Damanaki1Andressa Vilas Boas Nogueira2Sigrun Eick3Svenja Memmert4Xiaoyan Zhou5Shanika Nanayakkara6Werner Götz7Joni Augusto Cirelli8Andreas Jäger9James Deschner10Section of Experimental Dento-Maxillo-Facial Medicine, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, GermanySection of Experimental Dento-Maxillo-Facial Medicine, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, GermanyDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, São Paulo State University (UNESP), Araraquara, SP, BrazilDepartment of Periodontology, Laboratory of Oral Microbiology, University of Bern, Bern, SwitzerlandSection of Experimental Dento-Maxillo-Facial Medicine, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, GermanyFaculty of Dentistry, University of Sydney, Sydney, AustraliaFaculty of Dentistry, University of Sydney, Sydney, AustraliaDepartment of Orthodontics, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, GermanyDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, São Paulo State University (UNESP), Araraquara, SP, BrazilDepartment of Orthodontics, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, GermanySection of Experimental Dento-Maxillo-Facial Medicine, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, GermanyGhrelin plays a major role in obesity-related diseases which have been shown to be associated with periodontitis. This study sought to analyze the expression of the functional receptor for ghrelin (GHS-R1a) in periodontal cells and tissues under microbial conditions in vitro and in vivo. The GHS-R1a expression in human periodontal cells challenged with the periodontopathogen Fusobacterium nucleatum, in gingival biopsies from periodontally healthy and diseased individuals, and from rats with and without ligature-induced periodontitis was analyzed by real-time PCR, immunocytochemistry, and immunofluorescence. F. nucleatum induced an initial upregulation and subsequent downregulation of GHS-R1a in periodontal cells. In rat experimental periodontitis, the GHS-R1a expression at periodontitis sites was increased during the early stage of periodontitis, but significantly reduced afterwards, when compared with healthy sites. In human gingival biopsies, periodontally diseased sites showed a significantly lower GHS-R1a expression than the healthy sites. The expression of the functional ghrelin receptor in periodontal cells and tissues is modulated by periodontal bacteria. Due to the downregulation of the functional ghrelin receptor by long-term exposure to periodontal bacteria, the anti-inflammatory actions of ghrelin may be diminished in chronic periodontal infections, which could lead to an enhanced periodontal inflammation and tissue destruction.http://dx.doi.org/10.1155/2017/4916971 |
| spellingShingle | Marjan Nokhbehsaim Anna Damanaki Andressa Vilas Boas Nogueira Sigrun Eick Svenja Memmert Xiaoyan Zhou Shanika Nanayakkara Werner Götz Joni Augusto Cirelli Andreas Jäger James Deschner Regulation of Ghrelin Receptor by Periodontal Bacteria In Vitro and In Vivo Mediators of Inflammation |
| title | Regulation of Ghrelin Receptor by Periodontal Bacteria In Vitro and In Vivo |
| title_full | Regulation of Ghrelin Receptor by Periodontal Bacteria In Vitro and In Vivo |
| title_fullStr | Regulation of Ghrelin Receptor by Periodontal Bacteria In Vitro and In Vivo |
| title_full_unstemmed | Regulation of Ghrelin Receptor by Periodontal Bacteria In Vitro and In Vivo |
| title_short | Regulation of Ghrelin Receptor by Periodontal Bacteria In Vitro and In Vivo |
| title_sort | regulation of ghrelin receptor by periodontal bacteria in vitro and in vivo |
| url | http://dx.doi.org/10.1155/2017/4916971 |
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