Genomic insights into multidrug-resistant Acinetobacter baumannii strains isolated from Dhaka, Bangladesh
Acinetobacter baumannii is one of the most common nosocomial infections causing pathogens. Multidrug-resistant (MDR) A. baumannii is a growing concern to the healthcare system in Bangladesh, necessitating the elucidation of the genetic characteristics of this species. Five MDR, biofilm-forming A. ba...
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Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology
2025-03-01
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| Series: | Journal of Advanced Biotechnology and Experimental Therapeutics |
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| Online Access: | https://www.bsmiab.org/jabet/?mno=220373 |
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| author | Sadia Ferdous K M Shayerul Abedin Shayer Afia Anjum Sabbir R. Shuvo Ishrat Jabeen Kristi Paul Hridi A.K.M. Imrul Hassan Md Imran Khan Mohammad Fazle Alam Rabbi Mahmud Hossain Kazi Nadim Hasan Sohidul Islam |
| author_facet | Sadia Ferdous K M Shayerul Abedin Shayer Afia Anjum Sabbir R. Shuvo Ishrat Jabeen Kristi Paul Hridi A.K.M. Imrul Hassan Md Imran Khan Mohammad Fazle Alam Rabbi Mahmud Hossain Kazi Nadim Hasan Sohidul Islam |
| author_sort | Sadia Ferdous |
| collection | DOAJ |
| description | Acinetobacter baumannii is one of the most common nosocomial infections causing pathogens. Multidrug-resistant (MDR) A. baumannii is a growing concern to the healthcare system in Bangladesh, necessitating the elucidation of the genetic characteristics of this species. Five MDR, biofilm-forming A. baumannii strains isolated from clinical samples in Dhaka, Bangladesh, underwent a comprehensive whole-genome shotgun sequencing. In silico analyses determined strain types, antibiotic resistance, and virulence genes. The phylogenomic and pan-genome comparative analysis included the study samples along with an additional 15 Bangladeshi A. baumannii strains collected from the public database. The antibiotic-resistant profile showed that all the strains were resistant to meropenem. Two strains were resistant to polymyxin B up to 64 µg/ml. The average draft genome size of the strains was 3.93 Mbp, with about 39% GC content. Multilocus sequence typing characterized the strains as ST1, ST2, ST164, and ST623. All strains carried different classes of β-lactamase genes, including the carbapenem hydrolyzing enzyme coding genes blaoxa-23 and blaoxa-51. One of the five strains studied harbored blaNDM-9 and blaNDM-23 genes. The phosphotransferase group of aminoglycosides modifying enzymes was the most common. All the strains had relatively similar virulence profiles. Pan-genome analysis revealed 1944 core and 1302 unique genes. Phylogenomic analysis revealed the distribution of these strains in different clusters with other MDR strains isolated worldwide. The study results suggest the implementation of antibiotic stewardship and genomic surveillance of MDR A. baumannii to control the spread of antibiotic resistance in Bangladesh. [ J Adv Biotechnol Exp Ther 2025; 8(1.000): 64-78] |
| format | Article |
| id | doaj-art-5b565e88e86e4048b1a865eea8e3efaf |
| institution | Kabale University |
| issn | 2616-4760 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology |
| record_format | Article |
| series | Journal of Advanced Biotechnology and Experimental Therapeutics |
| spelling | doaj-art-5b565e88e86e4048b1a865eea8e3efaf2025-01-30T13:01:08ZengBangladesh Society for Microbiology, Immunology, and Advanced BiotechnologyJournal of Advanced Biotechnology and Experimental Therapeutics2616-47602025-03-0181647810.5455/jabet.2025.06220373Genomic insights into multidrug-resistant Acinetobacter baumannii strains isolated from Dhaka, BangladeshSadia Ferdous0K M Shayerul Abedin Shayer1Afia Anjum2Sabbir R. Shuvo3Ishrat Jabeen4Kristi Paul Hridi5A.K.M. Imrul Hassan6Md Imran Khan7Mohammad Fazle Alam Rabbi8Mahmud Hossain9Kazi Nadim Hasan10Sohidul Islam11Department of Biochemistry & Microbiology, North South University, Dhaka, Bangladesh Department of Biochemistry & Microbiology, North South University, Dhaka, Bangladesh Department of Biochemistry & Microbiology, North South University, Dhaka, Bangladesh Department of Biochemistry & Microbiology, North South University, Dhaka, Bangladesh Department of Biochemistry & Microbiology, North South University, Dhaka, Bangladesh Department of Biochemistry & Microbiology, North South University, Dhaka, Bangladesh Department of Biochemistry & Microbiology, North South University, Dhaka, Bangladesh Department of Genetics, Genomics and Bioinformatics, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, USA a. Department of Soil, Water and Environment, University of Dhaka, Dhaka-1000, Bangladesh b. DNA Solution Ltd, Dhaka, Bangladesh Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-1000, Bangladesh Department of Biochemistry & Microbiology, North South University, Dhaka, BangladeshAcinetobacter baumannii is one of the most common nosocomial infections causing pathogens. Multidrug-resistant (MDR) A. baumannii is a growing concern to the healthcare system in Bangladesh, necessitating the elucidation of the genetic characteristics of this species. Five MDR, biofilm-forming A. baumannii strains isolated from clinical samples in Dhaka, Bangladesh, underwent a comprehensive whole-genome shotgun sequencing. In silico analyses determined strain types, antibiotic resistance, and virulence genes. The phylogenomic and pan-genome comparative analysis included the study samples along with an additional 15 Bangladeshi A. baumannii strains collected from the public database. The antibiotic-resistant profile showed that all the strains were resistant to meropenem. Two strains were resistant to polymyxin B up to 64 µg/ml. The average draft genome size of the strains was 3.93 Mbp, with about 39% GC content. Multilocus sequence typing characterized the strains as ST1, ST2, ST164, and ST623. All strains carried different classes of β-lactamase genes, including the carbapenem hydrolyzing enzyme coding genes blaoxa-23 and blaoxa-51. One of the five strains studied harbored blaNDM-9 and blaNDM-23 genes. The phosphotransferase group of aminoglycosides modifying enzymes was the most common. All the strains had relatively similar virulence profiles. Pan-genome analysis revealed 1944 core and 1302 unique genes. Phylogenomic analysis revealed the distribution of these strains in different clusters with other MDR strains isolated worldwide. The study results suggest the implementation of antibiotic stewardship and genomic surveillance of MDR A. baumannii to control the spread of antibiotic resistance in Bangladesh. [ J Adv Biotechnol Exp Ther 2025; 8(1.000): 64-78]https://www.bsmiab.org/jabet/?mno=220373acinetobacter baumanniimultidrug resistancebangladeshgenomics |
| spellingShingle | Sadia Ferdous K M Shayerul Abedin Shayer Afia Anjum Sabbir R. Shuvo Ishrat Jabeen Kristi Paul Hridi A.K.M. Imrul Hassan Md Imran Khan Mohammad Fazle Alam Rabbi Mahmud Hossain Kazi Nadim Hasan Sohidul Islam Genomic insights into multidrug-resistant Acinetobacter baumannii strains isolated from Dhaka, Bangladesh Journal of Advanced Biotechnology and Experimental Therapeutics acinetobacter baumannii multidrug resistance bangladesh genomics |
| title | Genomic insights into multidrug-resistant Acinetobacter baumannii strains isolated from Dhaka, Bangladesh |
| title_full | Genomic insights into multidrug-resistant Acinetobacter baumannii strains isolated from Dhaka, Bangladesh |
| title_fullStr | Genomic insights into multidrug-resistant Acinetobacter baumannii strains isolated from Dhaka, Bangladesh |
| title_full_unstemmed | Genomic insights into multidrug-resistant Acinetobacter baumannii strains isolated from Dhaka, Bangladesh |
| title_short | Genomic insights into multidrug-resistant Acinetobacter baumannii strains isolated from Dhaka, Bangladesh |
| title_sort | genomic insights into multidrug resistant acinetobacter baumannii strains isolated from dhaka bangladesh |
| topic | acinetobacter baumannii multidrug resistance bangladesh genomics |
| url | https://www.bsmiab.org/jabet/?mno=220373 |
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