Optimization of Medium Composition and Fluidized Bed Drying Conditions for Efficient Production of Dry Yeast
Yeast formulations such as dry yeast are essential for supplying microbial starters to the alcoholic beverage industry. In Korea, the expensive freeze-drying method is used to manufacture brewer’s dry yeast, and therefore an economical process such as fluidized bed drying is needed. In the dry yeast...
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MDPI AG
2024-12-01
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| Series: | Microorganisms |
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| author | Hyun-Jin Kang Hwan Hee Yu Chang-Won Cho Young Kyung Rhee Tae-Wan Kim Young-Wook Chin |
| author_facet | Hyun-Jin Kang Hwan Hee Yu Chang-Won Cho Young Kyung Rhee Tae-Wan Kim Young-Wook Chin |
| author_sort | Hyun-Jin Kang |
| collection | DOAJ |
| description | Yeast formulations such as dry yeast are essential for supplying microbial starters to the alcoholic beverage industry. In Korea, the expensive freeze-drying method is used to manufacture brewer’s dry yeast, and therefore an economical process such as fluidized bed drying is needed. In the dry yeast manufacturing process, the medium and drying conditions are key factors that determine its quality and manufacturing cost. In this study, we aimed to optimize the medium composition and fluidized bed drying conditions for the efficient production of dry yeast. Muscovado and corn steep liquor were used as the carbon and nitrogen sources, respectively, and their optimal concentrations were identified using response surface methodology for efficient cultivation of <i>Saccharomyces cerevisiae</i> ReY4-7 isolated from <i>nuruk</i>. Central composite design analysis revealed that the optimal medium composition was 146.12 g/L muscovado and 58.68 g/L corn steep liquor. A dry cell weight of 36 g/L was achieved during 24 h of batch fermentation in a 30-L bioreactor containing this medium. Analysis of protective agents against fluidized bed drying revealed Span 80 as the strongest protective agent for <i>S. cerevisiae</i> ReY4-7. Response surface methodology revealed 50 °C and 41.45 min as the optimal fluidized bed drying conditions, under which the viable cell count reached 10.28 log CFU/g, comparable to that of the commercial dry yeast products. Overall, optimization of the medium and drying conditions significantly improved the final cell concentration in the cultivation process and the viable cell count in the drying process of dry yeast. |
| format | Article |
| id | doaj-art-5b13f8bcb7944b659c1bb5d3f23c3f87 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-5b13f8bcb7944b659c1bb5d3f23c3f872025-01-24T13:42:19ZengMDPI AGMicroorganisms2076-26072024-12-011312210.3390/microorganisms13010022Optimization of Medium Composition and Fluidized Bed Drying Conditions for Efficient Production of Dry YeastHyun-Jin Kang0Hwan Hee Yu1Chang-Won Cho2Young Kyung Rhee3Tae-Wan Kim4Young-Wook Chin5Research Group of Traditional Food, Korea Food Research Institute, Iseo-myeon, Wanju-gun 55365, Jeollabuk-do, Republic of KoreaFood Standard Research Center, Korea Food Research Institute, Iseo-myeon, Wanju-gun 55365, Jeollabuk-do, Republic of KoreaResearch Group of Traditional Food, Korea Food Research Institute, Iseo-myeon, Wanju-gun 55365, Jeollabuk-do, Republic of KoreaResearch Group of Traditional Food, Korea Food Research Institute, Iseo-myeon, Wanju-gun 55365, Jeollabuk-do, Republic of KoreaResearch Group of Traditional Food, Korea Food Research Institute, Iseo-myeon, Wanju-gun 55365, Jeollabuk-do, Republic of KoreaResearch Group of Traditional Food, Korea Food Research Institute, Iseo-myeon, Wanju-gun 55365, Jeollabuk-do, Republic of KoreaYeast formulations such as dry yeast are essential for supplying microbial starters to the alcoholic beverage industry. In Korea, the expensive freeze-drying method is used to manufacture brewer’s dry yeast, and therefore an economical process such as fluidized bed drying is needed. In the dry yeast manufacturing process, the medium and drying conditions are key factors that determine its quality and manufacturing cost. In this study, we aimed to optimize the medium composition and fluidized bed drying conditions for the efficient production of dry yeast. Muscovado and corn steep liquor were used as the carbon and nitrogen sources, respectively, and their optimal concentrations were identified using response surface methodology for efficient cultivation of <i>Saccharomyces cerevisiae</i> ReY4-7 isolated from <i>nuruk</i>. Central composite design analysis revealed that the optimal medium composition was 146.12 g/L muscovado and 58.68 g/L corn steep liquor. A dry cell weight of 36 g/L was achieved during 24 h of batch fermentation in a 30-L bioreactor containing this medium. Analysis of protective agents against fluidized bed drying revealed Span 80 as the strongest protective agent for <i>S. cerevisiae</i> ReY4-7. Response surface methodology revealed 50 °C and 41.45 min as the optimal fluidized bed drying conditions, under which the viable cell count reached 10.28 log CFU/g, comparable to that of the commercial dry yeast products. Overall, optimization of the medium and drying conditions significantly improved the final cell concentration in the cultivation process and the viable cell count in the drying process of dry yeast.https://www.mdpi.com/2076-2607/13/1/22dry yeastfluidized bed dryingmedium compositionresponse surface methodologybatch fermentation |
| spellingShingle | Hyun-Jin Kang Hwan Hee Yu Chang-Won Cho Young Kyung Rhee Tae-Wan Kim Young-Wook Chin Optimization of Medium Composition and Fluidized Bed Drying Conditions for Efficient Production of Dry Yeast Microorganisms dry yeast fluidized bed drying medium composition response surface methodology batch fermentation |
| title | Optimization of Medium Composition and Fluidized Bed Drying Conditions for Efficient Production of Dry Yeast |
| title_full | Optimization of Medium Composition and Fluidized Bed Drying Conditions for Efficient Production of Dry Yeast |
| title_fullStr | Optimization of Medium Composition and Fluidized Bed Drying Conditions for Efficient Production of Dry Yeast |
| title_full_unstemmed | Optimization of Medium Composition and Fluidized Bed Drying Conditions for Efficient Production of Dry Yeast |
| title_short | Optimization of Medium Composition and Fluidized Bed Drying Conditions for Efficient Production of Dry Yeast |
| title_sort | optimization of medium composition and fluidized bed drying conditions for efficient production of dry yeast |
| topic | dry yeast fluidized bed drying medium composition response surface methodology batch fermentation |
| url | https://www.mdpi.com/2076-2607/13/1/22 |
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