Nitrate Reductase Inhibition Induces Lipid Enhancement of Dunaliella Tertiolecta for Biodiesel Production
Nitrogen stress increases lipids content in microalgae, the main feedstock for algal biodiesel. Sodium tungstate was used in this study to implement nitrogen stress by inhibiting nitrate reductase (NR) in Dunaliella tertiolecta. The reduction of NR activity was accompanied by reduction of chlorophyl...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2018/6834725 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832549576259665920 |
|---|---|
| author | Redouane Benhima Hicham El Arroussi Issam M. Kadmiri Najib El Mernissi Imane Wahby Iman Bennis AbdelAziz Smouni Najib Bendaou |
| author_facet | Redouane Benhima Hicham El Arroussi Issam M. Kadmiri Najib El Mernissi Imane Wahby Iman Bennis AbdelAziz Smouni Najib Bendaou |
| author_sort | Redouane Benhima |
| collection | DOAJ |
| description | Nitrogen stress increases lipids content in microalgae, the main feedstock for algal biodiesel. Sodium tungstate was used in this study to implement nitrogen stress by inhibiting nitrate reductase (NR) in Dunaliella tertiolecta. The reduction of NR activity was accompanied by reduction of chlorophyll and accumulation of lipids. One-stage and two-stage culture strategies were compared. One-stage culture raised total lipids from 18% (control) to 39% (w: w); however, two-stage culture raised lipids to 50% in which neutral lipids were enhanced 2.14 times. To assess the quality of biodiesel produced, fatty acid methyl esters (FAME) composition was studied. It showed a slight variation of unsaturation. In addition, some physical proprieties of biodiesel were estimated and showed that higher heating values were improved by tungstate treatment. In this study, we tried to shed light on some biological impact of NR inhibition in microalgae cells using sodium tungstate which could be exploited in the improvement of biodiesel production. |
| format | Article |
| id | doaj-art-5ad73d3d902144a6a75ab7565c4d423a |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-5ad73d3d902144a6a75ab7565c4d423a2025-02-03T06:10:53ZengWileyThe Scientific World Journal2356-61401537-744X2018-01-01201810.1155/2018/68347256834725Nitrate Reductase Inhibition Induces Lipid Enhancement of Dunaliella Tertiolecta for Biodiesel ProductionRedouane Benhima0Hicham El Arroussi1Issam M. Kadmiri2Najib El Mernissi3Imane Wahby4Iman Bennis5AbdelAziz Smouni6Najib Bendaou7Green Biotechnology Centre, Moroccan Foundation for Advanced Science Innovation and Research, MoroccoGreen Biotechnology Centre, Moroccan Foundation for Advanced Science Innovation and Research, MoroccoGreen Biotechnology Centre, Moroccan Foundation for Advanced Science Innovation and Research, MoroccoGreen Biotechnology Centre, Moroccan Foundation for Advanced Science Innovation and Research, MoroccoGreen Biotechnology Centre, Moroccan Foundation for Advanced Science Innovation and Research, MoroccoGreen Biotechnology Centre, Moroccan Foundation for Advanced Science Innovation and Research, MoroccoLaboratoire de Physiologie et Biotechnologie Végétale, Faculté des Sciences, Mohammed V University, MoroccoLaboratoire de Physiologie et Biotechnologie Végétale, Faculté des Sciences, Mohammed V University, MoroccoNitrogen stress increases lipids content in microalgae, the main feedstock for algal biodiesel. Sodium tungstate was used in this study to implement nitrogen stress by inhibiting nitrate reductase (NR) in Dunaliella tertiolecta. The reduction of NR activity was accompanied by reduction of chlorophyll and accumulation of lipids. One-stage and two-stage culture strategies were compared. One-stage culture raised total lipids from 18% (control) to 39% (w: w); however, two-stage culture raised lipids to 50% in which neutral lipids were enhanced 2.14 times. To assess the quality of biodiesel produced, fatty acid methyl esters (FAME) composition was studied. It showed a slight variation of unsaturation. In addition, some physical proprieties of biodiesel were estimated and showed that higher heating values were improved by tungstate treatment. In this study, we tried to shed light on some biological impact of NR inhibition in microalgae cells using sodium tungstate which could be exploited in the improvement of biodiesel production.http://dx.doi.org/10.1155/2018/6834725 |
| spellingShingle | Redouane Benhima Hicham El Arroussi Issam M. Kadmiri Najib El Mernissi Imane Wahby Iman Bennis AbdelAziz Smouni Najib Bendaou Nitrate Reductase Inhibition Induces Lipid Enhancement of Dunaliella Tertiolecta for Biodiesel Production The Scientific World Journal |
| title | Nitrate Reductase Inhibition Induces Lipid Enhancement of Dunaliella Tertiolecta for Biodiesel Production |
| title_full | Nitrate Reductase Inhibition Induces Lipid Enhancement of Dunaliella Tertiolecta for Biodiesel Production |
| title_fullStr | Nitrate Reductase Inhibition Induces Lipid Enhancement of Dunaliella Tertiolecta for Biodiesel Production |
| title_full_unstemmed | Nitrate Reductase Inhibition Induces Lipid Enhancement of Dunaliella Tertiolecta for Biodiesel Production |
| title_short | Nitrate Reductase Inhibition Induces Lipid Enhancement of Dunaliella Tertiolecta for Biodiesel Production |
| title_sort | nitrate reductase inhibition induces lipid enhancement of dunaliella tertiolecta for biodiesel production |
| url | http://dx.doi.org/10.1155/2018/6834725 |
| work_keys_str_mv | AT redouanebenhima nitratereductaseinhibitioninduceslipidenhancementofdunaliellatertiolectaforbiodieselproduction AT hichamelarroussi nitratereductaseinhibitioninduceslipidenhancementofdunaliellatertiolectaforbiodieselproduction AT issammkadmiri nitratereductaseinhibitioninduceslipidenhancementofdunaliellatertiolectaforbiodieselproduction AT najibelmernissi nitratereductaseinhibitioninduceslipidenhancementofdunaliellatertiolectaforbiodieselproduction AT imanewahby nitratereductaseinhibitioninduceslipidenhancementofdunaliellatertiolectaforbiodieselproduction AT imanbennis nitratereductaseinhibitioninduceslipidenhancementofdunaliellatertiolectaforbiodieselproduction AT abdelazizsmouni nitratereductaseinhibitioninduceslipidenhancementofdunaliellatertiolectaforbiodieselproduction AT najibbendaou nitratereductaseinhibitioninduceslipidenhancementofdunaliellatertiolectaforbiodieselproduction |