R-Loop Formation In Trans at an AGGAG Repeat

Formation of RNA-DNA hybrid, or R-loop, was studied in vitro by transcribing an AGGAG repeat with T7 RNA polymerase. When ribonuclease T1 was present, R-loop formation in cis was diminished, indicating that the transcript was separated from the template and reassociated with it. The transcript was f...

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Bibliographic Details
Main Authors: Kazuya Toriumi, Takuma Tsukahara, Ryo Hanai
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:Journal of Nucleic Acids
Online Access:http://dx.doi.org/10.1155/2013/629218
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