R-Loop Formation In Trans at an AGGAG Repeat
Formation of RNA-DNA hybrid, or R-loop, was studied in vitro by transcribing an AGGAG repeat with T7 RNA polymerase. When ribonuclease T1 was present, R-loop formation in cis was diminished, indicating that the transcript was separated from the template and reassociated with it. The transcript was f...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Nucleic Acids |
| Online Access: | http://dx.doi.org/10.1155/2013/629218 |
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| _version_ | 1832551949235388416 |
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| author | Kazuya Toriumi Takuma Tsukahara Ryo Hanai |
| author_facet | Kazuya Toriumi Takuma Tsukahara Ryo Hanai |
| author_sort | Kazuya Toriumi |
| collection | DOAJ |
| description | Formation of RNA-DNA hybrid, or R-loop, was studied in vitro by transcribing an AGGAG repeat with T7 RNA polymerase. When ribonuclease T1 was present, R-loop formation in cis was diminished, indicating that the transcript was separated from the template and reassociated with it. The transcript was found to form an R-loop in trans with DNA comprising the AGGAG repeat, when the DNA was supercoiled. Results of chemical modification indicated that the duplex opened at the AGGAG repeat under negative supercoiling. |
| format | Article |
| id | doaj-art-5acf9de78f0c4c368e272608ae9b7fc6 |
| institution | Kabale University |
| issn | 2090-0201 2090-021X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nucleic Acids |
| spelling | doaj-art-5acf9de78f0c4c368e272608ae9b7fc62025-02-03T06:00:04ZengWileyJournal of Nucleic Acids2090-02012090-021X2013-01-01201310.1155/2013/629218629218R-Loop Formation In Trans at an AGGAG RepeatKazuya Toriumi0Takuma Tsukahara1Ryo Hanai2Department of Life Science and Research Center for Life Science, Rikkyo (St. Paul’s) University, 3-34-1 Nishi-Ikebukuro, Toshima-ku, Tokyo 171-8501, JapanDepartment of Life Science and Research Center for Life Science, Rikkyo (St. Paul’s) University, 3-34-1 Nishi-Ikebukuro, Toshima-ku, Tokyo 171-8501, JapanDepartment of Life Science and Research Center for Life Science, Rikkyo (St. Paul’s) University, 3-34-1 Nishi-Ikebukuro, Toshima-ku, Tokyo 171-8501, JapanFormation of RNA-DNA hybrid, or R-loop, was studied in vitro by transcribing an AGGAG repeat with T7 RNA polymerase. When ribonuclease T1 was present, R-loop formation in cis was diminished, indicating that the transcript was separated from the template and reassociated with it. The transcript was found to form an R-loop in trans with DNA comprising the AGGAG repeat, when the DNA was supercoiled. Results of chemical modification indicated that the duplex opened at the AGGAG repeat under negative supercoiling.http://dx.doi.org/10.1155/2013/629218 |
| spellingShingle | Kazuya Toriumi Takuma Tsukahara Ryo Hanai R-Loop Formation In Trans at an AGGAG Repeat Journal of Nucleic Acids |
| title | R-Loop Formation In Trans at an AGGAG Repeat |
| title_full | R-Loop Formation In Trans at an AGGAG Repeat |
| title_fullStr | R-Loop Formation In Trans at an AGGAG Repeat |
| title_full_unstemmed | R-Loop Formation In Trans at an AGGAG Repeat |
| title_short | R-Loop Formation In Trans at an AGGAG Repeat |
| title_sort | r loop formation in trans at an aggag repeat |
| url | http://dx.doi.org/10.1155/2013/629218 |
| work_keys_str_mv | AT kazuyatoriumi rloopformationintransatanaggagrepeat AT takumatsukahara rloopformationintransatanaggagrepeat AT ryohanai rloopformationintransatanaggagrepeat |