In silico design of an epitope-based vaccine ensemble for fasliolopsiasis
IntroductionFasciolopsiasis, a food-borne intestinal disease is most common in Asia and the Indian subcontinent. Pigs are the reservoir host, and fasciolopsiasis is most widespread in locations where pigs are reared and aquatic plants are widely consumed. Human infection has been most commonly docum...
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Frontiers Media S.A.
2025-01-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2024.1451853/full |
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| author | Ruchishree Konhar Ruchishree Konhar Kanhu Charan Das Kanhu Charan Das Aiboklang Nongrum Rohan Raj Samal Shailesh Kumar Sarangi Devendra Kumar Biswal Devendra Kumar Biswal |
| author_facet | Ruchishree Konhar Ruchishree Konhar Kanhu Charan Das Kanhu Charan Das Aiboklang Nongrum Rohan Raj Samal Shailesh Kumar Sarangi Devendra Kumar Biswal Devendra Kumar Biswal |
| author_sort | Ruchishree Konhar |
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| description | IntroductionFasciolopsiasis, a food-borne intestinal disease is most common in Asia and the Indian subcontinent. Pigs are the reservoir host, and fasciolopsiasis is most widespread in locations where pigs are reared and aquatic plants are widely consumed. Human infection has been most commonly documented in China, Bangladesh, Southeast Asia, and parts of India. It predominates in school-age children, and significant worm burdens are not uncommon. The causal organism is Fasciolopsis buski, a giant intestinal fluke that infects humans and causes diarrhoea, fever, ascites, and intestinal blockage. The increasing prevalence of medication resistance and the necessity for an effective vaccination make controlling these diseases challenging.MethodsOver the last decade, we have achieved major advances in our understanding of intestinal fluke biology by in-depth interrogation and analysis of evolving F. buski omics datasets. The creation of large omics datasets for F. buski by our group has accelerated the discovery of key molecules involved in intestinal fluke biology, toxicity, and virulence that can be targeted for vaccine development. Finding successful vaccination antigen combinations from these huge number of genes/proteins in the available omics datasets is the key in combating these neglected tropical diseases. In the present study, we developed an in silico workflow to select antigens for composing a chimeric vaccine, which could be a significant technique for developing a fasciolopsiasis vaccine that prevents the parasite from causing serious harm.Results and discussionThis chimeric vaccine can now be tested experimentally and compared to other vaccine candidates to determine its potential influence on human health. Although the results are encouraging, additional validation is needed both in vivo and in vitro. Considering the extensive genetic data available for intestinal flukes that has expanded with technological advancements, we may need to reassess our methods and suggest a more sophisticated technique in the future for identifying vaccine molecules. |
| format | Article |
| id | doaj-art-5ac30327dd1e41738e6dd3ac7cb1aa20 |
| institution | Kabale University |
| issn | 1664-8021 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Genetics |
| spelling | doaj-art-5ac30327dd1e41738e6dd3ac7cb1aa202025-01-22T07:11:08ZengFrontiers Media S.A.Frontiers in Genetics1664-80212025-01-011510.3389/fgene.2024.14518531451853In silico design of an epitope-based vaccine ensemble for fasliolopsiasisRuchishree Konhar0Ruchishree Konhar1Kanhu Charan Das2Kanhu Charan Das3Aiboklang Nongrum4Rohan Raj Samal5Shailesh Kumar Sarangi6Devendra Kumar Biswal7Devendra Kumar Biswal8Informatics and Big Data, CSIR-Institute of Genomics and Integrative Biology, Delhi, IndiaAcademy of Scientific and Innovative Research, Ghaziabad, Uttar Pradesh, IndiaDepartment of Zoology, North-Eastern Hill University, Shillong, Meghalaya, IndiaBioinformatics Centre, North-Eastern Hill University, Shillong, Meghalaya, IndiaDepartment of Zoology, North-Eastern Hill University, Shillong, Meghalaya, IndiaBioinformatics Centre, North-Eastern Hill University, Shillong, Meghalaya, IndiaBioinformatics Centre, North-Eastern Hill University, Shillong, Meghalaya, IndiaDepartment of Zoology, North-Eastern Hill University, Shillong, Meghalaya, IndiaBioinformatics Centre, North-Eastern Hill University, Shillong, Meghalaya, IndiaIntroductionFasciolopsiasis, a food-borne intestinal disease is most common in Asia and the Indian subcontinent. Pigs are the reservoir host, and fasciolopsiasis is most widespread in locations where pigs are reared and aquatic plants are widely consumed. Human infection has been most commonly documented in China, Bangladesh, Southeast Asia, and parts of India. It predominates in school-age children, and significant worm burdens are not uncommon. The causal organism is Fasciolopsis buski, a giant intestinal fluke that infects humans and causes diarrhoea, fever, ascites, and intestinal blockage. The increasing prevalence of medication resistance and the necessity for an effective vaccination make controlling these diseases challenging.MethodsOver the last decade, we have achieved major advances in our understanding of intestinal fluke biology by in-depth interrogation and analysis of evolving F. buski omics datasets. The creation of large omics datasets for F. buski by our group has accelerated the discovery of key molecules involved in intestinal fluke biology, toxicity, and virulence that can be targeted for vaccine development. Finding successful vaccination antigen combinations from these huge number of genes/proteins in the available omics datasets is the key in combating these neglected tropical diseases. In the present study, we developed an in silico workflow to select antigens for composing a chimeric vaccine, which could be a significant technique for developing a fasciolopsiasis vaccine that prevents the parasite from causing serious harm.Results and discussionThis chimeric vaccine can now be tested experimentally and compared to other vaccine candidates to determine its potential influence on human health. Although the results are encouraging, additional validation is needed both in vivo and in vitro. Considering the extensive genetic data available for intestinal flukes that has expanded with technological advancements, we may need to reassess our methods and suggest a more sophisticated technique in the future for identifying vaccine molecules.https://www.frontiersin.org/articles/10.3389/fgene.2024.1451853/fullFasciolopsis buskiTLR2immune simulationintestinal flukevaccine construct |
| spellingShingle | Ruchishree Konhar Ruchishree Konhar Kanhu Charan Das Kanhu Charan Das Aiboklang Nongrum Rohan Raj Samal Shailesh Kumar Sarangi Devendra Kumar Biswal Devendra Kumar Biswal In silico design of an epitope-based vaccine ensemble for fasliolopsiasis Frontiers in Genetics Fasciolopsis buski TLR2 immune simulation intestinal fluke vaccine construct |
| title | In silico design of an epitope-based vaccine ensemble for fasliolopsiasis |
| title_full | In silico design of an epitope-based vaccine ensemble for fasliolopsiasis |
| title_fullStr | In silico design of an epitope-based vaccine ensemble for fasliolopsiasis |
| title_full_unstemmed | In silico design of an epitope-based vaccine ensemble for fasliolopsiasis |
| title_short | In silico design of an epitope-based vaccine ensemble for fasliolopsiasis |
| title_sort | in silico design of an epitope based vaccine ensemble for fasliolopsiasis |
| topic | Fasciolopsis buski TLR2 immune simulation intestinal fluke vaccine construct |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2024.1451853/full |
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