Critical steps and troubleshooting in sample preparation for wood and phloem formation: from sampling to microscopic observation
We present a technical note that supplements published procedures on optimal sample preparation for performing wood and phloem formation analyses. Before beginning sampling, it is important to learn about the characteristics of the tree or shrub species to be investigated. Some tips are given how t...
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2022-07-01
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| Series: | Les |
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| Online Access: | https://journals.uni-lj.si/les-wood/article/view/11606 |
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| author | Angela Balzano Katarina Čufar Luka Krže Maks Merela |
| author_facet | Angela Balzano Katarina Čufar Luka Krže Maks Merela |
| author_sort | Angela Balzano |
| collection | DOAJ |
| description |
We present a technical note that supplements published procedures on optimal sample preparation for performing wood and phloem formation analyses. Before beginning sampling, it is important to learn about the characteristics of the tree or shrub species to be investigated. Some tips are given how to use the Trephor tool in the best way, how to remove the outer hard bark (periderm), how microcores should be handled after removal from the tree, and how they should be oriented for embedding in paraffin, and cutting thin sections for microscopy. Possible defects that may result from improper handling are illustrated and discussed. We also present optimal images to accurately identify different cell development stages in phloem and xylem, which is particularly challenging in hardwoods and Mediterranean tree and shrub species.
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| format | Article |
| id | doaj-art-5ab60596956144d586956af9129bd407 |
| institution | Kabale University |
| issn | 0024-1067 2590-9932 |
| language | English |
| publishDate | 2022-07-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Les |
| spelling | doaj-art-5ab60596956144d586956af9129bd4072025-02-06T15:19:27ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Les0024-10672590-99322022-07-0171110.26614/les-wood.2022.v71n01a05Critical steps and troubleshooting in sample preparation for wood and phloem formation: from sampling to microscopic observationAngela BalzanoKatarina ČufarLuka KržeMaks Merela We present a technical note that supplements published procedures on optimal sample preparation for performing wood and phloem formation analyses. Before beginning sampling, it is important to learn about the characteristics of the tree or shrub species to be investigated. Some tips are given how to use the Trephor tool in the best way, how to remove the outer hard bark (periderm), how microcores should be handled after removal from the tree, and how they should be oriented for embedding in paraffin, and cutting thin sections for microscopy. Possible defects that may result from improper handling are illustrated and discussed. We also present optimal images to accurately identify different cell development stages in phloem and xylem, which is particularly challenging in hardwoods and Mediterranean tree and shrub species. https://journals.uni-lj.si/les-wood/article/view/11606tissue samplingtissue preparationmicroscopic slidesmicroscopyxylogenesisphloem formation |
| spellingShingle | Angela Balzano Katarina Čufar Luka Krže Maks Merela Critical steps and troubleshooting in sample preparation for wood and phloem formation: from sampling to microscopic observation Les tissue sampling tissue preparation microscopic slides microscopy xylogenesis phloem formation |
| title | Critical steps and troubleshooting in sample preparation for wood and phloem formation: from sampling to microscopic observation |
| title_full | Critical steps and troubleshooting in sample preparation for wood and phloem formation: from sampling to microscopic observation |
| title_fullStr | Critical steps and troubleshooting in sample preparation for wood and phloem formation: from sampling to microscopic observation |
| title_full_unstemmed | Critical steps and troubleshooting in sample preparation for wood and phloem formation: from sampling to microscopic observation |
| title_short | Critical steps and troubleshooting in sample preparation for wood and phloem formation: from sampling to microscopic observation |
| title_sort | critical steps and troubleshooting in sample preparation for wood and phloem formation from sampling to microscopic observation |
| topic | tissue sampling tissue preparation microscopic slides microscopy xylogenesis phloem formation |
| url | https://journals.uni-lj.si/les-wood/article/view/11606 |
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