Genome-wide characterization of the MADS-box gene family in Paeonia ostii and expression analysis of genes related to floral organ development
Abstract Background Paeonia section Moutan DC. is a significant perennial subshrub, the ornamental value of which heavily depends on the type of flower it possesses. MADS-box transcription factors have a particular impact on the intricate process of floral organ development and differentiation. The...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-01-01
|
| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-024-11197-y |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832585988753326080 |
|---|---|
| author | Xueting Yang Qi Guo Shaodan Liu Duoduo Wang Dingding Zuo Tongfei Niu Dongfeng Wei Lili Guo Xiaogai Hou |
| author_facet | Xueting Yang Qi Guo Shaodan Liu Duoduo Wang Dingding Zuo Tongfei Niu Dongfeng Wei Lili Guo Xiaogai Hou |
| author_sort | Xueting Yang |
| collection | DOAJ |
| description | Abstract Background Paeonia section Moutan DC. is a significant perennial subshrub, the ornamental value of which heavily depends on the type of flower it possesses. MADS-box transcription factors have a particular impact on the intricate process of floral organ development and differentiation. The release of the whole-genome data from Paeonia ostii now allows us to conduct a thorough investigation of the tree peony MADS-box gene family. Results In this study, we identified 110 MADS-box genes in Paeonia ostii that were classified into 5 subgroups. Gene structure, domain and motif analyses revealed the conservation of the structure of these subgroups. Analysis of the cis-acting elements revealed that the 110 PoMADS genes contained different kinds of hormones and stress-related cis-acting elements in their promoter regions. Quantitative real-time PCR analysis was employed to validate the expression patterns of some PoMADS genes related to floral organ development. Genome collinearity analysis with Arabidopsis and grape revealed the conservation of PoMADS genes during evolution. A total of 857 SSRs were identified by analysing the genome sequences of identified genes. We additionally created protein‒protein interaction networks for PoMADS proteins and analysed proteins that could interact among PoMADSs in Arabidopsis thaliana and grape. Conclusion These findings offer fundamental insights for understanding the function of the MADS-box gene family, which can aid in the selection and breeding of tree peony varieties with high ornamental value in addition to supporting the understanding of the process of tree peony floral organogenesis. |
| format | Article |
| id | doaj-art-5a9fef9666254b5fb7a12eb1daa37218 |
| institution | Kabale University |
| issn | 1471-2164 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj-art-5a9fef9666254b5fb7a12eb1daa372182025-01-26T12:16:38ZengBMCBMC Genomics1471-21642025-01-0126111710.1186/s12864-024-11197-yGenome-wide characterization of the MADS-box gene family in Paeonia ostii and expression analysis of genes related to floral organ developmentXueting Yang0Qi Guo1Shaodan Liu2Duoduo Wang3Dingding Zuo4Tongfei Niu5Dongfeng Wei6Lili Guo7Xiaogai Hou8College of Agriculture / Tree Peony, Henan University of Science and TechnologyCollege of Agriculture / Tree Peony, Henan University of Science and TechnologyLuoyang International Peony GardenCollege of Agriculture / Tree Peony, Henan University of Science and TechnologyCollege of Agriculture / Tree Peony, Henan University of Science and TechnologyCollege of Agriculture / Tree Peony, Henan University of Science and TechnologyLuoyang Vocational and Technical CollegeCollege of Agriculture / Tree Peony, Henan University of Science and TechnologyCollege of Agriculture / Tree Peony, Henan University of Science and TechnologyAbstract Background Paeonia section Moutan DC. is a significant perennial subshrub, the ornamental value of which heavily depends on the type of flower it possesses. MADS-box transcription factors have a particular impact on the intricate process of floral organ development and differentiation. The release of the whole-genome data from Paeonia ostii now allows us to conduct a thorough investigation of the tree peony MADS-box gene family. Results In this study, we identified 110 MADS-box genes in Paeonia ostii that were classified into 5 subgroups. Gene structure, domain and motif analyses revealed the conservation of the structure of these subgroups. Analysis of the cis-acting elements revealed that the 110 PoMADS genes contained different kinds of hormones and stress-related cis-acting elements in their promoter regions. Quantitative real-time PCR analysis was employed to validate the expression patterns of some PoMADS genes related to floral organ development. Genome collinearity analysis with Arabidopsis and grape revealed the conservation of PoMADS genes during evolution. A total of 857 SSRs were identified by analysing the genome sequences of identified genes. We additionally created protein‒protein interaction networks for PoMADS proteins and analysed proteins that could interact among PoMADSs in Arabidopsis thaliana and grape. Conclusion These findings offer fundamental insights for understanding the function of the MADS-box gene family, which can aid in the selection and breeding of tree peony varieties with high ornamental value in addition to supporting the understanding of the process of tree peony floral organogenesis.https://doi.org/10.1186/s12864-024-11197-yFloral organTree peonyMADS-boxExpression analysis |
| spellingShingle | Xueting Yang Qi Guo Shaodan Liu Duoduo Wang Dingding Zuo Tongfei Niu Dongfeng Wei Lili Guo Xiaogai Hou Genome-wide characterization of the MADS-box gene family in Paeonia ostii and expression analysis of genes related to floral organ development BMC Genomics Floral organ Tree peony MADS-box Expression analysis |
| title | Genome-wide characterization of the MADS-box gene family in Paeonia ostii and expression analysis of genes related to floral organ development |
| title_full | Genome-wide characterization of the MADS-box gene family in Paeonia ostii and expression analysis of genes related to floral organ development |
| title_fullStr | Genome-wide characterization of the MADS-box gene family in Paeonia ostii and expression analysis of genes related to floral organ development |
| title_full_unstemmed | Genome-wide characterization of the MADS-box gene family in Paeonia ostii and expression analysis of genes related to floral organ development |
| title_short | Genome-wide characterization of the MADS-box gene family in Paeonia ostii and expression analysis of genes related to floral organ development |
| title_sort | genome wide characterization of the mads box gene family in paeonia ostii and expression analysis of genes related to floral organ development |
| topic | Floral organ Tree peony MADS-box Expression analysis |
| url | https://doi.org/10.1186/s12864-024-11197-y |
| work_keys_str_mv | AT xuetingyang genomewidecharacterizationofthemadsboxgenefamilyinpaeoniaostiiandexpressionanalysisofgenesrelatedtofloralorgandevelopment AT qiguo genomewidecharacterizationofthemadsboxgenefamilyinpaeoniaostiiandexpressionanalysisofgenesrelatedtofloralorgandevelopment AT shaodanliu genomewidecharacterizationofthemadsboxgenefamilyinpaeoniaostiiandexpressionanalysisofgenesrelatedtofloralorgandevelopment AT duoduowang genomewidecharacterizationofthemadsboxgenefamilyinpaeoniaostiiandexpressionanalysisofgenesrelatedtofloralorgandevelopment AT dingdingzuo genomewidecharacterizationofthemadsboxgenefamilyinpaeoniaostiiandexpressionanalysisofgenesrelatedtofloralorgandevelopment AT tongfeiniu genomewidecharacterizationofthemadsboxgenefamilyinpaeoniaostiiandexpressionanalysisofgenesrelatedtofloralorgandevelopment AT dongfengwei genomewidecharacterizationofthemadsboxgenefamilyinpaeoniaostiiandexpressionanalysisofgenesrelatedtofloralorgandevelopment AT liliguo genomewidecharacterizationofthemadsboxgenefamilyinpaeoniaostiiandexpressionanalysisofgenesrelatedtofloralorgandevelopment AT xiaogaihou genomewidecharacterizationofthemadsboxgenefamilyinpaeoniaostiiandexpressionanalysisofgenesrelatedtofloralorgandevelopment |