Modulation of CXC Chemokine Receptor Expression and Function in Human Neutrophils during Aging In Vitro Suggests a Role in Their Clearance from Circulation
In mice, differential regulation of CXC chemokine receptor expression in circulating polymorphonuclear neutrophils (PMNs) undergoing senescence results in homing to the bone marrow. However, the role of this compartment and of the chemokine receptor CXCR4 is still under discussion, and only scarce d...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2009/790174 |
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| author | Katja C. Weisel Frank Bautz Gabriele Seitz Sedat Yildirim Lothar Kanz Robert Möhle |
| author_facet | Katja C. Weisel Frank Bautz Gabriele Seitz Sedat Yildirim Lothar Kanz Robert Möhle |
| author_sort | Katja C. Weisel |
| collection | DOAJ |
| description | In mice, differential regulation of CXC chemokine receptor expression in circulating polymorphonuclear neutrophils (PMNs) undergoing senescence results in homing to the bone marrow. However, the role of this compartment and of the chemokine receptor
CXCR4 is still under discussion, and only scarce data exist about CXCR4 function in
human PMN. In our study, we provide evidence that also in human neutrophils, expression
(cell surface and mRNA), chemotactic and signaling functions of the homing-related
chemokine receptor CXCR4 are upregulated during aging in vitro, independent of addition
of stimulatory cytokines (TNF, IL-1, IL-8, G-CSF). In contrast, interleukin-8 receptors are
downmodulated (CXCR2) or remain unchanged (CXCR1), suggesting that human PMNs
undergoing senescence acquire a phenotype that impairs inflammatory extravasation and
favors homing to the bone marrow or other tissues involved in sequestration. Partially
retained responsiveness to interleukin-8 may be important for neutrophil function when
senescence occurs after extravasation in inflamed tissues. |
| format | Article |
| id | doaj-art-5a93a1707a2a4f65b3609cbfb3bdd468 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-5a93a1707a2a4f65b3609cbfb3bdd4682025-02-03T05:52:33ZengWileyMediators of Inflammation0962-93511466-18612009-01-01200910.1155/2009/790174790174Modulation of CXC Chemokine Receptor Expression and Function in Human Neutrophils during Aging In Vitro Suggests a Role in Their Clearance from CirculationKatja C. Weisel0Frank Bautz1Gabriele Seitz2Sedat Yildirim3Lothar Kanz4Robert Möhle5Department of Medicine II, University of Tübingen, Otfried-Müller-Street 10, 72076 Tübingen, GermanyDepartment of Medicine II, University of Tübingen, Otfried-Müller-Street 10, 72076 Tübingen, GermanyDepartment of Medicine II, University of Tübingen, Otfried-Müller-Street 10, 72076 Tübingen, GermanyDepartment of Medicine II, University of Tübingen, Otfried-Müller-Street 10, 72076 Tübingen, GermanyDepartment of Medicine II, University of Tübingen, Otfried-Müller-Street 10, 72076 Tübingen, GermanyDepartment of Medicine II, University of Tübingen, Otfried-Müller-Street 10, 72076 Tübingen, GermanyIn mice, differential regulation of CXC chemokine receptor expression in circulating polymorphonuclear neutrophils (PMNs) undergoing senescence results in homing to the bone marrow. However, the role of this compartment and of the chemokine receptor CXCR4 is still under discussion, and only scarce data exist about CXCR4 function in human PMN. In our study, we provide evidence that also in human neutrophils, expression (cell surface and mRNA), chemotactic and signaling functions of the homing-related chemokine receptor CXCR4 are upregulated during aging in vitro, independent of addition of stimulatory cytokines (TNF, IL-1, IL-8, G-CSF). In contrast, interleukin-8 receptors are downmodulated (CXCR2) or remain unchanged (CXCR1), suggesting that human PMNs undergoing senescence acquire a phenotype that impairs inflammatory extravasation and favors homing to the bone marrow or other tissues involved in sequestration. Partially retained responsiveness to interleukin-8 may be important for neutrophil function when senescence occurs after extravasation in inflamed tissues.http://dx.doi.org/10.1155/2009/790174 |
| spellingShingle | Katja C. Weisel Frank Bautz Gabriele Seitz Sedat Yildirim Lothar Kanz Robert Möhle Modulation of CXC Chemokine Receptor Expression and Function in Human Neutrophils during Aging In Vitro Suggests a Role in Their Clearance from Circulation Mediators of Inflammation |
| title | Modulation of CXC Chemokine Receptor Expression and Function in Human Neutrophils during Aging In Vitro Suggests a Role in Their Clearance from Circulation |
| title_full | Modulation of CXC Chemokine Receptor Expression and Function in Human Neutrophils during Aging In Vitro Suggests a Role in Their Clearance from Circulation |
| title_fullStr | Modulation of CXC Chemokine Receptor Expression and Function in Human Neutrophils during Aging In Vitro Suggests a Role in Their Clearance from Circulation |
| title_full_unstemmed | Modulation of CXC Chemokine Receptor Expression and Function in Human Neutrophils during Aging In Vitro Suggests a Role in Their Clearance from Circulation |
| title_short | Modulation of CXC Chemokine Receptor Expression and Function in Human Neutrophils during Aging In Vitro Suggests a Role in Their Clearance from Circulation |
| title_sort | modulation of cxc chemokine receptor expression and function in human neutrophils during aging in vitro suggests a role in their clearance from circulation |
| url | http://dx.doi.org/10.1155/2009/790174 |
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