Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient
Ectopic thyroid results from a migration defect of the developing gland during embryogenesis causing congenital hypothyroidism. But it has also been detected in asymptomatic individuals. This study aimed to investigate the histopathological, functional, and genetic features of human ectopic thyroids...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2018/4682876 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832554219684495360 |
|---|---|
| author | Rosalinda Yasato Camargo Cristina Takami Kanamura Celso Ubirajara Friguglietti Célia Regina Nogueira Sonia Iorcansky Alfio José Tincani Ana Karina Bezerra Ester Brust Fernanda Christtanini Koyama Anamaria Aranha Camargo Fernanda Orpinelli R. Rego Pedro Alexandre Favoretto Galante Geraldo Medeiros-Neto Ileana Gabriela Sanchez Rubio |
| author_facet | Rosalinda Yasato Camargo Cristina Takami Kanamura Celso Ubirajara Friguglietti Célia Regina Nogueira Sonia Iorcansky Alfio José Tincani Ana Karina Bezerra Ester Brust Fernanda Christtanini Koyama Anamaria Aranha Camargo Fernanda Orpinelli R. Rego Pedro Alexandre Favoretto Galante Geraldo Medeiros-Neto Ileana Gabriela Sanchez Rubio |
| author_sort | Rosalinda Yasato Camargo |
| collection | DOAJ |
| description | Ectopic thyroid results from a migration defect of the developing gland during embryogenesis causing congenital hypothyroidism. But it has also been detected in asymptomatic individuals. This study aimed to investigate the histopathological, functional, and genetic features of human ectopic thyroids. Six samples were histologically examined, and the expression of the specific thyroid proteins was assessed by immunohistochemistry. Two samples were submitted to whole exome sequencing. An oropharynx sample showed immature fetal architecture tissue with clusters or cords of oval thyrocytes and small follicles; one sample exhibited a normal thyroid pattern while four showed colloid goiter. All ectopic thyroids expressed the specific thyroid genes and T4 at similar locations to those observed in normal thyroid. No somatic mutations associated with ectopic thyroid were found. This is the first immature thyroid fetal tissue observed in an ectopic thyroid due to the arrest of structural differentiation early in the colloid stage of development that proved able to synthesize thyroid hormone but not to respond to TSH. Despite the ability of all ectopic thyroids to synthetize specific thyroid proteins and T4, at some point in life, it may be insufficient to support body growth leading to hypothyroidism, as observed in some of the patients. |
| format | Article |
| id | doaj-art-5a62004b32e44bc9a1f7582f1da35ae3 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-5a62004b32e44bc9a1f7582f1da35ae32025-02-03T05:52:01ZengWileyInternational Journal of Endocrinology1687-83371687-83452018-01-01201810.1155/2018/46828764682876Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult PatientRosalinda Yasato Camargo0Cristina Takami Kanamura1Celso Ubirajara Friguglietti2Célia Regina Nogueira3Sonia Iorcansky4Alfio José Tincani5Ana Karina Bezerra6Ester Brust7Fernanda Christtanini Koyama8Anamaria Aranha Camargo9Fernanda Orpinelli R. Rego10Pedro Alexandre Favoretto Galante11Geraldo Medeiros-Neto12Ileana Gabriela Sanchez Rubio13Thyroid Unit, Cellular and Molecular Endocrine Laboratory, LIM-25, Faculdade de Medicina da Universidade de São Paulo (FMUSP), Avenida Doutor Arnaldo 455, Cerqueira César, 01246-904 São Paulo, SP, BrazilAdolfo Lutz Institute, São Paulo Public Health Service, Av. Dr. Arnaldo 355, Cerqueira César, 01246-000 São Paulo, SP, BrazilHead and Neck Surgery of Santa Catarina Hospital, Av. Paulista 200, Bela Vista, 01310-000 São Paulo, SP, BrazilDepartment of Internal Medicine, Botucatu School of Medicine, UNESP, Av. Prof. Montenegro, s/n Distrito de Rubião Junior, 18618-687 Botucatu, SP, BrazilServicio de Endocrinología, Hospital de Pediatría Dr. Juan Garrahan, Combate de los Pozos 1881, C1245AAM Buenos Aires, ArgentinaDepartamento de Cirurgia na Disciplina de Cirurgia de Cabeça e Pescoço da Faculdade de Ciências Médicas da UNICAMP, R. Tessália Vieira de Camargo 126, 13083-887 Campinas, SP, BrazilMedicine School, Universidade de Fortaleza (Unifor), Av. Washington Soares 1321, Edson Queiroz, 60811-905 Fortaleza, CE, BrazilPostgraduate Program in Biotechnology, Universidade Federal de São Paulo (UNIFESP), Pedro de Toledo 669, 040399-032 São Paulo, SP, BrazilMolecular Oncology Center, Hospital Sírio-Libanés, Rua Prof. Daher Cutait 69, 01308-060 São Paulo, SP, BrazilMolecular Oncology Center, Hospital Sírio-Libanés, Rua Prof. Daher Cutait 69, 01308-060 São Paulo, SP, BrazilMolecular Oncology Center, Hospital Sírio-Libanés, Rua Prof. Daher Cutait 69, 01308-060 São Paulo, SP, BrazilMolecular Oncology Center, Hospital Sírio-Libanés, Rua Prof. Daher Cutait 69, 01308-060 São Paulo, SP, BrazilThyroid Unit, Cellular and Molecular Endocrine Laboratory, LIM-25, Faculdade de Medicina da Universidade de São Paulo (FMUSP), Avenida Doutor Arnaldo 455, Cerqueira César, 01246-904 São Paulo, SP, BrazilPostgraduate Program in Biotechnology, Universidade Federal de São Paulo (UNIFESP), Pedro de Toledo 669, 040399-032 São Paulo, SP, BrazilEctopic thyroid results from a migration defect of the developing gland during embryogenesis causing congenital hypothyroidism. But it has also been detected in asymptomatic individuals. This study aimed to investigate the histopathological, functional, and genetic features of human ectopic thyroids. Six samples were histologically examined, and the expression of the specific thyroid proteins was assessed by immunohistochemistry. Two samples were submitted to whole exome sequencing. An oropharynx sample showed immature fetal architecture tissue with clusters or cords of oval thyrocytes and small follicles; one sample exhibited a normal thyroid pattern while four showed colloid goiter. All ectopic thyroids expressed the specific thyroid genes and T4 at similar locations to those observed in normal thyroid. No somatic mutations associated with ectopic thyroid were found. This is the first immature thyroid fetal tissue observed in an ectopic thyroid due to the arrest of structural differentiation early in the colloid stage of development that proved able to synthesize thyroid hormone but not to respond to TSH. Despite the ability of all ectopic thyroids to synthetize specific thyroid proteins and T4, at some point in life, it may be insufficient to support body growth leading to hypothyroidism, as observed in some of the patients.http://dx.doi.org/10.1155/2018/4682876 |
| spellingShingle | Rosalinda Yasato Camargo Cristina Takami Kanamura Celso Ubirajara Friguglietti Célia Regina Nogueira Sonia Iorcansky Alfio José Tincani Ana Karina Bezerra Ester Brust Fernanda Christtanini Koyama Anamaria Aranha Camargo Fernanda Orpinelli R. Rego Pedro Alexandre Favoretto Galante Geraldo Medeiros-Neto Ileana Gabriela Sanchez Rubio Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient International Journal of Endocrinology |
| title | Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient |
| title_full | Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient |
| title_fullStr | Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient |
| title_full_unstemmed | Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient |
| title_short | Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient |
| title_sort | histopathological characterization and whole exome sequencing of ectopic thyroid fetal architecture in a functional ectopic gland from adult patient |
| url | http://dx.doi.org/10.1155/2018/4682876 |
| work_keys_str_mv | AT rosalindayasatocamargo histopathologicalcharacterizationandwholeexomesequencingofectopicthyroidfetalarchitectureinafunctionalectopicglandfromadultpatient AT cristinatakamikanamura histopathologicalcharacterizationandwholeexomesequencingofectopicthyroidfetalarchitectureinafunctionalectopicglandfromadultpatient AT celsoubirajarafriguglietti histopathologicalcharacterizationandwholeexomesequencingofectopicthyroidfetalarchitectureinafunctionalectopicglandfromadultpatient AT celiareginanogueira histopathologicalcharacterizationandwholeexomesequencingofectopicthyroidfetalarchitectureinafunctionalectopicglandfromadultpatient AT soniaiorcansky histopathologicalcharacterizationandwholeexomesequencingofectopicthyroidfetalarchitectureinafunctionalectopicglandfromadultpatient AT alfiojosetincani histopathologicalcharacterizationandwholeexomesequencingofectopicthyroidfetalarchitectureinafunctionalectopicglandfromadultpatient AT anakarinabezerra histopathologicalcharacterizationandwholeexomesequencingofectopicthyroidfetalarchitectureinafunctionalectopicglandfromadultpatient AT esterbrust histopathologicalcharacterizationandwholeexomesequencingofectopicthyroidfetalarchitectureinafunctionalectopicglandfromadultpatient AT fernandachristtaninikoyama histopathologicalcharacterizationandwholeexomesequencingofectopicthyroidfetalarchitectureinafunctionalectopicglandfromadultpatient AT anamariaaranhacamargo histopathologicalcharacterizationandwholeexomesequencingofectopicthyroidfetalarchitectureinafunctionalectopicglandfromadultpatient AT fernandaorpinellirrego histopathologicalcharacterizationandwholeexomesequencingofectopicthyroidfetalarchitectureinafunctionalectopicglandfromadultpatient AT pedroalexandrefavorettogalante histopathologicalcharacterizationandwholeexomesequencingofectopicthyroidfetalarchitectureinafunctionalectopicglandfromadultpatient AT geraldomedeirosneto histopathologicalcharacterizationandwholeexomesequencingofectopicthyroidfetalarchitectureinafunctionalectopicglandfromadultpatient AT ileanagabrielasanchezrubio histopathologicalcharacterizationandwholeexomesequencingofectopicthyroidfetalarchitectureinafunctionalectopicglandfromadultpatient |