Pathogenicity and Whole Genome Sequence Analysis of a Pseudorabies Virus Strain FJ-2012 Isolated from Fujian, Southern China
The outbreaks of pseudorabies have been frequently reported in Bartha-K61-vaccinated farms in China since 2011. To study the pathogenicity and evolution of the circulating pseudorabies viruses in Fujian Province, mainland China, we isolated and sequenced the whole genome of a wild-type pseudorabies...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2017/9073172 |
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| author | Xue-min Wu Qiu-yong Chen Ru-jing Chen Yong-liang Che Long-bai Wang Chen-yan Wang Shan Yan Yu-tao Liu Jin-sheng Xiu Lun-jiang Zhou |
| author_facet | Xue-min Wu Qiu-yong Chen Ru-jing Chen Yong-liang Che Long-bai Wang Chen-yan Wang Shan Yan Yu-tao Liu Jin-sheng Xiu Lun-jiang Zhou |
| author_sort | Xue-min Wu |
| collection | DOAJ |
| description | The outbreaks of pseudorabies have been frequently reported in Bartha-K61-vaccinated farms in China since 2011. To study the pathogenicity and evolution of the circulating pseudorabies viruses in Fujian Province, mainland China, we isolated and sequenced the whole genome of a wild-type pseudorabies virus strain named “FJ-2012.” We then conducted a few downstream bioinformatics analyses including phylogenetic analysis and pathogenic analysis and used the virus to infect 6 pseudorabies virus-free piglets. FJ-2012-infected piglets developed symptoms like high body temperature and central nervous system disorders and had high mortality rate. In addition, we identified typical micropathological changes such as multiple gross lesions in infected piglets through pathological analysis and conclude that the FJ-2012 genome is significantly different from known pseudorabies viruses, in which insertions, deletions, and substitutions are observed in multiple immune and virulence genes. In summary, this study shed lights on the molecular basis of the prevalence and pathology of the pseudorabies virus strain FJ-2012. The genome of FJ-2012 could be used as a reference to study the evolution of pseudorabies viruses, which is critical to the vaccine development of new emerging pseudorabies viruses. |
| format | Article |
| id | doaj-art-5a39f2f7bbb2459086de189127b90a02 |
| institution | Kabale University |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-5a39f2f7bbb2459086de189127b90a022025-02-03T05:45:48ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932017-01-01201710.1155/2017/90731729073172Pathogenicity and Whole Genome Sequence Analysis of a Pseudorabies Virus Strain FJ-2012 Isolated from Fujian, Southern ChinaXue-min Wu0Qiu-yong Chen1Ru-jing Chen2Yong-liang Che3Long-bai Wang4Chen-yan Wang5Shan Yan6Yu-tao Liu7Jin-sheng Xiu8Lun-jiang Zhou9Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences/Fujian Animal Disease Control Technology Development Center, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences/Fujian Animal Disease Control Technology Development Center, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences/Fujian Animal Disease Control Technology Development Center, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences/Fujian Animal Disease Control Technology Development Center, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences/Fujian Animal Disease Control Technology Development Center, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences/Fujian Animal Disease Control Technology Development Center, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences/Fujian Animal Disease Control Technology Development Center, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences/Fujian Animal Disease Control Technology Development Center, Fuzhou 350013, ChinaCollege of Animal Sciences, Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences/Fujian Animal Disease Control Technology Development Center, Fuzhou 350013, ChinaThe outbreaks of pseudorabies have been frequently reported in Bartha-K61-vaccinated farms in China since 2011. To study the pathogenicity and evolution of the circulating pseudorabies viruses in Fujian Province, mainland China, we isolated and sequenced the whole genome of a wild-type pseudorabies virus strain named “FJ-2012.” We then conducted a few downstream bioinformatics analyses including phylogenetic analysis and pathogenic analysis and used the virus to infect 6 pseudorabies virus-free piglets. FJ-2012-infected piglets developed symptoms like high body temperature and central nervous system disorders and had high mortality rate. In addition, we identified typical micropathological changes such as multiple gross lesions in infected piglets through pathological analysis and conclude that the FJ-2012 genome is significantly different from known pseudorabies viruses, in which insertions, deletions, and substitutions are observed in multiple immune and virulence genes. In summary, this study shed lights on the molecular basis of the prevalence and pathology of the pseudorabies virus strain FJ-2012. The genome of FJ-2012 could be used as a reference to study the evolution of pseudorabies viruses, which is critical to the vaccine development of new emerging pseudorabies viruses.http://dx.doi.org/10.1155/2017/9073172 |
| spellingShingle | Xue-min Wu Qiu-yong Chen Ru-jing Chen Yong-liang Che Long-bai Wang Chen-yan Wang Shan Yan Yu-tao Liu Jin-sheng Xiu Lun-jiang Zhou Pathogenicity and Whole Genome Sequence Analysis of a Pseudorabies Virus Strain FJ-2012 Isolated from Fujian, Southern China Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Pathogenicity and Whole Genome Sequence Analysis of a Pseudorabies Virus Strain FJ-2012 Isolated from Fujian, Southern China |
| title_full | Pathogenicity and Whole Genome Sequence Analysis of a Pseudorabies Virus Strain FJ-2012 Isolated from Fujian, Southern China |
| title_fullStr | Pathogenicity and Whole Genome Sequence Analysis of a Pseudorabies Virus Strain FJ-2012 Isolated from Fujian, Southern China |
| title_full_unstemmed | Pathogenicity and Whole Genome Sequence Analysis of a Pseudorabies Virus Strain FJ-2012 Isolated from Fujian, Southern China |
| title_short | Pathogenicity and Whole Genome Sequence Analysis of a Pseudorabies Virus Strain FJ-2012 Isolated from Fujian, Southern China |
| title_sort | pathogenicity and whole genome sequence analysis of a pseudorabies virus strain fj 2012 isolated from fujian southern china |
| url | http://dx.doi.org/10.1155/2017/9073172 |
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