Exploring potentially synthetic genes related to diarrhetic shellfish toxins production in Prorocentrum sp. via comparative transcriptomics
Harmful algal blooms (HABs), exacerbated by climate change and environmental disturbances, pose global challenges due to marine toxin contamination, particularly diarrhetic shellfish toxins (DSTs). DSTs are prevalent marine toxins, and understanding their synthesis is vital for managing fisheries an...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325000314 |
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| author | Danlin Zheng Jingyi Cen Peiliang Chen Ligong Zou Jian Zou Qun Li Songhui Lu |
| author_facet | Danlin Zheng Jingyi Cen Peiliang Chen Ligong Zou Jian Zou Qun Li Songhui Lu |
| author_sort | Danlin Zheng |
| collection | DOAJ |
| description | Harmful algal blooms (HABs), exacerbated by climate change and environmental disturbances, pose global challenges due to marine toxin contamination, particularly diarrhetic shellfish toxins (DSTs). DSTs are prevalent marine toxins, and understanding their synthesis is vital for managing fisheries and mitigating environmental triggers. This study delves into the synthesis mechanisms of DSTs in Prorocentrum arenarium and Prorocentrum lima, which vary in toxin types and concentrations. We conducted a comprehensive comparative transcriptomic analysis to identify potential toxin-related genes, focusing on polyketide synthases (PKSs) and fatty acid synthases (FASs). Our research predicted 96 PKSs and 91 FASs genes, with a detailed examination of their sequences to elucidate dinophysistoxins (DTXs) synthesis. Additionally, we analyzed differential gene expression of PKSs in P. arenarium under nitrogen and phosphorus-limited conditions, revealing a correlation between specific PKSs gene expression patterns and okadaic acid (OA) content variations. These findings suggest a potential role of the fatty acid biosynthesis pathway in DSTs synthesis. While not completely uncovering the biosynthetic pathway of DSTs, our study offers crucial insights and genomic resources for future research on dinoflagellate toxin production mechanisms. |
| format | Article |
| id | doaj-art-59f06b95c9464cddb1572a61635333f8 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-59f06b95c9464cddb1572a61635333f82025-01-23T05:26:08ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117695Exploring potentially synthetic genes related to diarrhetic shellfish toxins production in Prorocentrum sp. via comparative transcriptomicsDanlin Zheng0Jingyi Cen1Peiliang Chen2Ligong Zou3Jian Zou4Qun Li5Songhui Lu6College of Life Science and Technology, and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Jinan University, Guangzhou 510362, ChinaCollege of Life Science and Technology, and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Jinan University, Guangzhou 510362, ChinaCollege of Life Science and Technology, and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Jinan University, Guangzhou 510362, ChinaCollege of Life Science and Technology, and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Jinan University, Guangzhou 510362, ChinaCollege of Life Science and Technology, and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Jinan University, Guangzhou 510362, ChinaCollege of Life Science and Technology, and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Jinan University, Guangzhou 510362, ChinaCorresponding author.; College of Life Science and Technology, and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Jinan University, Guangzhou 510362, ChinaHarmful algal blooms (HABs), exacerbated by climate change and environmental disturbances, pose global challenges due to marine toxin contamination, particularly diarrhetic shellfish toxins (DSTs). DSTs are prevalent marine toxins, and understanding their synthesis is vital for managing fisheries and mitigating environmental triggers. This study delves into the synthesis mechanisms of DSTs in Prorocentrum arenarium and Prorocentrum lima, which vary in toxin types and concentrations. We conducted a comprehensive comparative transcriptomic analysis to identify potential toxin-related genes, focusing on polyketide synthases (PKSs) and fatty acid synthases (FASs). Our research predicted 96 PKSs and 91 FASs genes, with a detailed examination of their sequences to elucidate dinophysistoxins (DTXs) synthesis. Additionally, we analyzed differential gene expression of PKSs in P. arenarium under nitrogen and phosphorus-limited conditions, revealing a correlation between specific PKSs gene expression patterns and okadaic acid (OA) content variations. These findings suggest a potential role of the fatty acid biosynthesis pathway in DSTs synthesis. While not completely uncovering the biosynthetic pathway of DSTs, our study offers crucial insights and genomic resources for future research on dinoflagellate toxin production mechanisms.http://www.sciencedirect.com/science/article/pii/S0147651325000314ProrocentrumTranscriptomic analysisDiarrhetic shellfish toxins (DSTs)Polyketide synthaseFatty acid synthase |
| spellingShingle | Danlin Zheng Jingyi Cen Peiliang Chen Ligong Zou Jian Zou Qun Li Songhui Lu Exploring potentially synthetic genes related to diarrhetic shellfish toxins production in Prorocentrum sp. via comparative transcriptomics Ecotoxicology and Environmental Safety Prorocentrum Transcriptomic analysis Diarrhetic shellfish toxins (DSTs) Polyketide synthase Fatty acid synthase |
| title | Exploring potentially synthetic genes related to diarrhetic shellfish toxins production in Prorocentrum sp. via comparative transcriptomics |
| title_full | Exploring potentially synthetic genes related to diarrhetic shellfish toxins production in Prorocentrum sp. via comparative transcriptomics |
| title_fullStr | Exploring potentially synthetic genes related to diarrhetic shellfish toxins production in Prorocentrum sp. via comparative transcriptomics |
| title_full_unstemmed | Exploring potentially synthetic genes related to diarrhetic shellfish toxins production in Prorocentrum sp. via comparative transcriptomics |
| title_short | Exploring potentially synthetic genes related to diarrhetic shellfish toxins production in Prorocentrum sp. via comparative transcriptomics |
| title_sort | exploring potentially synthetic genes related to diarrhetic shellfish toxins production in prorocentrum sp via comparative transcriptomics |
| topic | Prorocentrum Transcriptomic analysis Diarrhetic shellfish toxins (DSTs) Polyketide synthase Fatty acid synthase |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325000314 |
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