Role of Transforming Growth Factor-β1 and Smads Signaling Pathway in Intrauterine Adhesion
The aim of the study was to evaluate the role of Smad3, Smad7, and TGF-β1 in intrauterine adhesion (IUA) patients and experimental rabbit models. 60 IUA patients, 30 control participants, and 18 female rabbits were enrolled in this study. We found that the plasma concentrations and protein expressio...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/4158287 |
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| author | Umme Salma Min Xue Md Sayed Ali Sheikh Xiaoming Guan Bin Xu Aiqian Zhang Lihua Huang Dabao Xu |
| author_facet | Umme Salma Min Xue Md Sayed Ali Sheikh Xiaoming Guan Bin Xu Aiqian Zhang Lihua Huang Dabao Xu |
| author_sort | Umme Salma |
| collection | DOAJ |
| description | The aim of the study was to evaluate the role of Smad3, Smad7, and TGF-β1 in intrauterine adhesion (IUA) patients and experimental rabbit models. 60 IUA patients, 30 control participants, and 18 female rabbits were enrolled in this study. We found that the plasma concentrations and protein expressions of TGF-β1 were significantly increased in patients and experimental rabbits compared to those in controls (P<0.05). Furthermore, the mRNA and protein expression levels of Smad3 were significantly elevated, while Smad7 level was markedly decreased in the patients and experimental rabbits compared with controls (P<0.05). This altered ratio recommended that IUA was positively correlated to the mRNA and protein expression levels of Smad3, Smad7, and TGF-β1 in blood and uterine tissue. Moreover, we used the specific inhibitor of Smad3 (SIS3) in experimental rabbit. SIS3 obviously reduced the mRNA and protein expression of smad3 and TGF-β1, while it increased Smad7 expression in the treatment groups as compared with IUA rabbits (P<0.05). Our study suggested that TGF-β1/Smad3/smad7 is a major pathway which plays an important role in the regulation of the IUA and specific inhibitor of Smad3 (SIS3) may provide a new therapeutic strategy for IUA. |
| format | Article |
| id | doaj-art-597f03ea37ed47b1b424a07d8e3aefaf |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-597f03ea37ed47b1b424a07d8e3aefaf2025-02-03T07:24:05ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/41582874158287Role of Transforming Growth Factor-β1 and Smads Signaling Pathway in Intrauterine AdhesionUmme Salma0Min Xue1Md Sayed Ali Sheikh2Xiaoming Guan3Bin Xu4Aiqian Zhang5Lihua Huang6Dabao Xu7Department of Gynecology, Third Xiangya Hospital of Central South University, 138 Tongzipo Road, Changsha, Hunan 410013, ChinaDepartment of Gynecology, Third Xiangya Hospital of Central South University, 138 Tongzipo Road, Changsha, Hunan 410013, ChinaDepartment of Cardiology, Xiangya Hospital, Central South University, Changsha, Hunan, ChinaDepartment of OB/GYN, Baylor College of Medicine, 6651 Main Street, 10th Floor, Houston, TX 77030, USADepartment of Gynecology, Third Xiangya Hospital of Central South University, 138 Tongzipo Road, Changsha, Hunan 410013, ChinaDepartment of Gynecology, Third Xiangya Hospital of Central South University, 138 Tongzipo Road, Changsha, Hunan 410013, ChinaCenter for Medical Experiments, Third Xiangya Hospital, Central South University, Changsha, Hunan, ChinaDepartment of Gynecology, Third Xiangya Hospital of Central South University, 138 Tongzipo Road, Changsha, Hunan 410013, ChinaThe aim of the study was to evaluate the role of Smad3, Smad7, and TGF-β1 in intrauterine adhesion (IUA) patients and experimental rabbit models. 60 IUA patients, 30 control participants, and 18 female rabbits were enrolled in this study. We found that the plasma concentrations and protein expressions of TGF-β1 were significantly increased in patients and experimental rabbits compared to those in controls (P<0.05). Furthermore, the mRNA and protein expression levels of Smad3 were significantly elevated, while Smad7 level was markedly decreased in the patients and experimental rabbits compared with controls (P<0.05). This altered ratio recommended that IUA was positively correlated to the mRNA and protein expression levels of Smad3, Smad7, and TGF-β1 in blood and uterine tissue. Moreover, we used the specific inhibitor of Smad3 (SIS3) in experimental rabbit. SIS3 obviously reduced the mRNA and protein expression of smad3 and TGF-β1, while it increased Smad7 expression in the treatment groups as compared with IUA rabbits (P<0.05). Our study suggested that TGF-β1/Smad3/smad7 is a major pathway which plays an important role in the regulation of the IUA and specific inhibitor of Smad3 (SIS3) may provide a new therapeutic strategy for IUA.http://dx.doi.org/10.1155/2016/4158287 |
| spellingShingle | Umme Salma Min Xue Md Sayed Ali Sheikh Xiaoming Guan Bin Xu Aiqian Zhang Lihua Huang Dabao Xu Role of Transforming Growth Factor-β1 and Smads Signaling Pathway in Intrauterine Adhesion Mediators of Inflammation |
| title | Role of Transforming Growth Factor-β1 and Smads Signaling Pathway in Intrauterine Adhesion |
| title_full | Role of Transforming Growth Factor-β1 and Smads Signaling Pathway in Intrauterine Adhesion |
| title_fullStr | Role of Transforming Growth Factor-β1 and Smads Signaling Pathway in Intrauterine Adhesion |
| title_full_unstemmed | Role of Transforming Growth Factor-β1 and Smads Signaling Pathway in Intrauterine Adhesion |
| title_short | Role of Transforming Growth Factor-β1 and Smads Signaling Pathway in Intrauterine Adhesion |
| title_sort | role of transforming growth factor β1 and smads signaling pathway in intrauterine adhesion |
| url | http://dx.doi.org/10.1155/2016/4158287 |
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