Aspergilli Response to Benzalkonium Chloride and Novel-Synthesized Fullerenol/Benzalkonium Chloride Nanocomposite
A comprehensive comparative analysis of antifungal potential of benzalkonium chloride and newly synthesized fullerenol/benzalkonium chloride nanocomposite was conducted to assess the possible impact of carbon-based nanocarrier on antimicrobial properties of the commonly used biocide. Physical charac...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2015/109262 |
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| author | Nikola Unković Milica Ljaljević Grbić Miloš Stupar Jelena Vukojević Vesna Janković Danica Jović Aleksandar Djordjević |
| author_facet | Nikola Unković Milica Ljaljević Grbić Miloš Stupar Jelena Vukojević Vesna Janković Danica Jović Aleksandar Djordjević |
| author_sort | Nikola Unković |
| collection | DOAJ |
| description | A comprehensive comparative analysis of antifungal potential of benzalkonium chloride and newly synthesized fullerenol/benzalkonium chloride nanocomposite was conducted to assess the possible impact of carbon-based nanocarrier on antimicrobial properties of the commonly used biocide. Physical characterization of synthesized nanocomposite showed zeta potential of +37.4 mV and inhomogeneous particles size distribution, with nanocomposite particles’ dimensions within 30–143 nm and maximum number of particles at 44 nm. The effect of pure and fullerenol nanocarrier-bound biocide was evaluated in eight Aspergillus species. In mycelial growth assay, nanocomposite was more potent, as fungicidal effect of 1.04/0.6 μg mL−1 was obtained in all but one of the isolates (A. niger), while proportional concentration of pure biocide (0.6 μg mL−1) completely inhibited mycelial growth of only three Aspergillus species. However, conidia appear to be less susceptible to nanocomposite treatment, as lower fungistatic (MIC) and fungicidal (MFC) concentrations were obtained with biocide alone (MIC in range from 0.03 to 0.15 μg mL−1 and MFC from 0.075 to 0.45 μg mL−1). To a different degree, both substances stimulated aflatoxin B1 production and inhibited ochratoxin A synthesis. Very low mycelium biomass yield, in range from 1.0 to 3.0 mg dry weight, was documented in both biocide and nanocomposite enriched medium. |
| format | Article |
| id | doaj-art-591a2de2bbef485daa2d019fa4b82d59 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-591a2de2bbef485daa2d019fa4b82d592025-02-03T07:24:19ZengWileyThe Scientific World Journal2356-61401537-744X2015-01-01201510.1155/2015/109262109262Aspergilli Response to Benzalkonium Chloride and Novel-Synthesized Fullerenol/Benzalkonium Chloride NanocompositeNikola Unković0Milica Ljaljević Grbić1Miloš Stupar2Jelena Vukojević3Vesna Janković4Danica Jović5Aleksandar Djordjević6Department of Algology, Mycology and Lichenology, Faculty of Biology, University of Belgrade, Institute of Botany and Botanical Garden “Jevremovac”, Takovska 43, 11 000 Belgrade, SerbiaDepartment of Algology, Mycology and Lichenology, Faculty of Biology, University of Belgrade, Institute of Botany and Botanical Garden “Jevremovac”, Takovska 43, 11 000 Belgrade, SerbiaDepartment of Algology, Mycology and Lichenology, Faculty of Biology, University of Belgrade, Institute of Botany and Botanical Garden “Jevremovac”, Takovska 43, 11 000 Belgrade, SerbiaDepartment of Algology, Mycology and Lichenology, Faculty of Biology, University of Belgrade, Institute of Botany and Botanical Garden “Jevremovac”, Takovska 43, 11 000 Belgrade, SerbiaInstitute of Meat Hygiene and Technology, Kaćanskog 13, 11 000 Belgrade, SerbiaDepartment of Chemistry, Biochemistry and Environmental Protection, Faculty of Science, University of Novi Sad, Trg Dositeja Obradovića 3, 21000 Novi Sad, SerbiaDepartment of Chemistry, Biochemistry and Environmental Protection, Faculty of Science, University of Novi Sad, Trg Dositeja Obradovića 3, 21000 Novi Sad, SerbiaA comprehensive comparative analysis of antifungal potential of benzalkonium chloride and newly synthesized fullerenol/benzalkonium chloride nanocomposite was conducted to assess the possible impact of carbon-based nanocarrier on antimicrobial properties of the commonly used biocide. Physical characterization of synthesized nanocomposite showed zeta potential of +37.4 mV and inhomogeneous particles size distribution, with nanocomposite particles’ dimensions within 30–143 nm and maximum number of particles at 44 nm. The effect of pure and fullerenol nanocarrier-bound biocide was evaluated in eight Aspergillus species. In mycelial growth assay, nanocomposite was more potent, as fungicidal effect of 1.04/0.6 μg mL−1 was obtained in all but one of the isolates (A. niger), while proportional concentration of pure biocide (0.6 μg mL−1) completely inhibited mycelial growth of only three Aspergillus species. However, conidia appear to be less susceptible to nanocomposite treatment, as lower fungistatic (MIC) and fungicidal (MFC) concentrations were obtained with biocide alone (MIC in range from 0.03 to 0.15 μg mL−1 and MFC from 0.075 to 0.45 μg mL−1). To a different degree, both substances stimulated aflatoxin B1 production and inhibited ochratoxin A synthesis. Very low mycelium biomass yield, in range from 1.0 to 3.0 mg dry weight, was documented in both biocide and nanocomposite enriched medium.http://dx.doi.org/10.1155/2015/109262 |
| spellingShingle | Nikola Unković Milica Ljaljević Grbić Miloš Stupar Jelena Vukojević Vesna Janković Danica Jović Aleksandar Djordjević Aspergilli Response to Benzalkonium Chloride and Novel-Synthesized Fullerenol/Benzalkonium Chloride Nanocomposite The Scientific World Journal |
| title | Aspergilli Response to Benzalkonium Chloride and Novel-Synthesized Fullerenol/Benzalkonium Chloride Nanocomposite |
| title_full | Aspergilli Response to Benzalkonium Chloride and Novel-Synthesized Fullerenol/Benzalkonium Chloride Nanocomposite |
| title_fullStr | Aspergilli Response to Benzalkonium Chloride and Novel-Synthesized Fullerenol/Benzalkonium Chloride Nanocomposite |
| title_full_unstemmed | Aspergilli Response to Benzalkonium Chloride and Novel-Synthesized Fullerenol/Benzalkonium Chloride Nanocomposite |
| title_short | Aspergilli Response to Benzalkonium Chloride and Novel-Synthesized Fullerenol/Benzalkonium Chloride Nanocomposite |
| title_sort | aspergilli response to benzalkonium chloride and novel synthesized fullerenol benzalkonium chloride nanocomposite |
| url | http://dx.doi.org/10.1155/2015/109262 |
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