Thyme Essential Oil as a Potential Tool Against Common and Re-Emerging Foodborne Pathogens: Biocidal Effect on Bacterial Membrane Permeability
Over the past decade, foodborne diseases have become a significant public health concern, affecting millions of people globally. Major pathogens like <i>Salmonella</i> spp., <i>Escherichia coli</i>, <i>Listeria monocytogenes</i>, and <i>Staphylococcus aureus...
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2024-12-01
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| author | Martina Di Rosario Leonardo Continisio Giuseppe Mantova Francesca Carraturo Elena Scaglione Daniela Sateriale Giuseppina Forgione Chiara Pagliuca Caterina Pagliarulo Roberta Colicchio Mariateresa Vitiello Paola Salvatore |
| author_facet | Martina Di Rosario Leonardo Continisio Giuseppe Mantova Francesca Carraturo Elena Scaglione Daniela Sateriale Giuseppina Forgione Chiara Pagliuca Caterina Pagliarulo Roberta Colicchio Mariateresa Vitiello Paola Salvatore |
| author_sort | Martina Di Rosario |
| collection | DOAJ |
| description | Over the past decade, foodborne diseases have become a significant public health concern, affecting millions of people globally. Major pathogens like <i>Salmonella</i> spp., <i>Escherichia coli</i>, <i>Listeria monocytogenes</i>, and <i>Staphylococcus aureus</i> contaminate food and cause several infections. This study investigates the potential of thyme essential oil (Thy-EO) as a natural antimicrobial agent against most common and re-emerging foodborne bacteria, including <i>S. enterica</i>, <i>Yersinia enterocolitica</i>, and <i>L. monocytogenes</i>. Preliminary tests provided qualitative evidence of Thy-EO’s efficacy by evaluating its antibacterial activity through direct contact and vapor phase exposure. Then, the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were defined to quantitatively evaluate the bacteriostatic and bactericidal effects of Thy-EO, revealing a strong inhibitory effect against <i>S. enterica</i>, <i>Y. enterocolitica</i> and <i>L. monocytogenes</i>. Additionally, Thy-EO exerted rapid bactericidal kinetics characterized by the disruption of bacterial cell membrane integrity over time. Results highlight Thy-EO’s potential as an alternative antimicrobial agent, demonstrating that treatment with Thy-EO significantly and irreversibly affects the growth of the tested foodborne pathogens. |
| format | Article |
| id | doaj-art-58d6b54ad6814930a056c9e3f283c274 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-58d6b54ad6814930a056c9e3f283c2742025-01-24T13:42:23ZengMDPI AGMicroorganisms2076-26072024-12-011313710.3390/microorganisms13010037Thyme Essential Oil as a Potential Tool Against Common and Re-Emerging Foodborne Pathogens: Biocidal Effect on Bacterial Membrane PermeabilityMartina Di Rosario0Leonardo Continisio1Giuseppe Mantova2Francesca Carraturo3Elena Scaglione4Daniela Sateriale5Giuseppina Forgione6Chiara Pagliuca7Caterina Pagliarulo8Roberta Colicchio9Mariateresa Vitiello10Paola Salvatore11Department of Molecular Medicine and Medical Biotechnologies, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Molecular Medicine and Medical Biotechnologies, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Molecular Medicine and Medical Biotechnologies, University of Naples “Federico II”, 80131 Naples, ItalyPediatric Surgery Unit, Department of Transalational Medical Science, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Molecular Medicine and Medical Biotechnologies, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Science and Technology, University of Sannio, Via F. De Sanctis snc, 82100 Benevento, ItalyDepartment of Science and Technology, University of Sannio, Via F. De Sanctis snc, 82100 Benevento, ItalyDepartment of Molecular Medicine and Medical Biotechnologies, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Science and Technology, University of Sannio, Via F. De Sanctis snc, 82100 Benevento, ItalyDepartment of Molecular Medicine and Medical Biotechnologies, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Molecular Medicine and Medical Biotechnologies, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Molecular Medicine and Medical Biotechnologies, University of Naples “Federico II”, 80131 Naples, ItalyOver the past decade, foodborne diseases have become a significant public health concern, affecting millions of people globally. Major pathogens like <i>Salmonella</i> spp., <i>Escherichia coli</i>, <i>Listeria monocytogenes</i>, and <i>Staphylococcus aureus</i> contaminate food and cause several infections. This study investigates the potential of thyme essential oil (Thy-EO) as a natural antimicrobial agent against most common and re-emerging foodborne bacteria, including <i>S. enterica</i>, <i>Yersinia enterocolitica</i>, and <i>L. monocytogenes</i>. Preliminary tests provided qualitative evidence of Thy-EO’s efficacy by evaluating its antibacterial activity through direct contact and vapor phase exposure. Then, the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were defined to quantitatively evaluate the bacteriostatic and bactericidal effects of Thy-EO, revealing a strong inhibitory effect against <i>S. enterica</i>, <i>Y. enterocolitica</i> and <i>L. monocytogenes</i>. Additionally, Thy-EO exerted rapid bactericidal kinetics characterized by the disruption of bacterial cell membrane integrity over time. Results highlight Thy-EO’s potential as an alternative antimicrobial agent, demonstrating that treatment with Thy-EO significantly and irreversibly affects the growth of the tested foodborne pathogens.https://www.mdpi.com/2076-2607/13/1/37thyme essential oilantibacterial effectfoodborne pathogensbacterial membrane damage |
| spellingShingle | Martina Di Rosario Leonardo Continisio Giuseppe Mantova Francesca Carraturo Elena Scaglione Daniela Sateriale Giuseppina Forgione Chiara Pagliuca Caterina Pagliarulo Roberta Colicchio Mariateresa Vitiello Paola Salvatore Thyme Essential Oil as a Potential Tool Against Common and Re-Emerging Foodborne Pathogens: Biocidal Effect on Bacterial Membrane Permeability Microorganisms thyme essential oil antibacterial effect foodborne pathogens bacterial membrane damage |
| title | Thyme Essential Oil as a Potential Tool Against Common and Re-Emerging Foodborne Pathogens: Biocidal Effect on Bacterial Membrane Permeability |
| title_full | Thyme Essential Oil as a Potential Tool Against Common and Re-Emerging Foodborne Pathogens: Biocidal Effect on Bacterial Membrane Permeability |
| title_fullStr | Thyme Essential Oil as a Potential Tool Against Common and Re-Emerging Foodborne Pathogens: Biocidal Effect on Bacterial Membrane Permeability |
| title_full_unstemmed | Thyme Essential Oil as a Potential Tool Against Common and Re-Emerging Foodborne Pathogens: Biocidal Effect on Bacterial Membrane Permeability |
| title_short | Thyme Essential Oil as a Potential Tool Against Common and Re-Emerging Foodborne Pathogens: Biocidal Effect on Bacterial Membrane Permeability |
| title_sort | thyme essential oil as a potential tool against common and re emerging foodborne pathogens biocidal effect on bacterial membrane permeability |
| topic | thyme essential oil antibacterial effect foodborne pathogens bacterial membrane damage |
| url | https://www.mdpi.com/2076-2607/13/1/37 |
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