The Use of Porous Scaffold as a Tumor Model

Background. Human cancer is a three-dimensional (3D) structure consisting of neighboring cells, extracellular matrix, and blood vessels. It is therefore critical to mimic the cancer cells and their surrounding environment during in vitro study. Our aim was to establish a 3D cancer model using a synt...

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Main Authors: Mei Zhang, Philip Boughton, Barbara Rose, C. Soon Lee, Angela M. Hong
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:International Journal of Biomaterials
Online Access:http://dx.doi.org/10.1155/2013/396056
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author Mei Zhang
Philip Boughton
Barbara Rose
C. Soon Lee
Angela M. Hong
author_facet Mei Zhang
Philip Boughton
Barbara Rose
C. Soon Lee
Angela M. Hong
author_sort Mei Zhang
collection DOAJ
description Background. Human cancer is a three-dimensional (3D) structure consisting of neighboring cells, extracellular matrix, and blood vessels. It is therefore critical to mimic the cancer cells and their surrounding environment during in vitro study. Our aim was to establish a 3D cancer model using a synthetic composite scaffold. Methods. High-density low-volume seeding was used to promote attachment of a non-small-cell lung cancer cell line (NCI-H460) to scaffolds. Growth patterns in 3D culture were compared with those of monolayers. Immunohistochemistry was conducted to compare the expression of Ki67, CD44, and carbonic anhydrase IX. Results. NCI-H460 readily attached to the scaffold without surface pretreatment at a rate of 35% from a load of 1.5 × 106 cells. Most cells grew vertically to form clumps along the surface of the scaffold, and cell morphology resembled tissue origin; 2D cultures exhibited characteristics of adherent epithelial cancer cell lines. Expression patterns of Ki67, CD44, and CA IX varied markedly between 3D and monolayer cultures. Conclusions. The behavior of cancer cells in our 3D model is similar to tumor growth in vivo. This model will provide the basis for future study using 3D cancer culture.
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spelling doaj-art-58aa0bca87c74dde8d24e06333137e952025-02-03T06:14:18ZengWileyInternational Journal of Biomaterials1687-87871687-87952013-01-01201310.1155/2013/396056396056The Use of Porous Scaffold as a Tumor ModelMei Zhang0Philip Boughton1Barbara Rose2C. Soon Lee3Angela M. Hong4Department of Radiation Oncology, Royal Prince Alfred Hospital, Sydney, NSW 2050, AustraliaThe Institute of Biomedical Engineering and Technology, The University of Sydney, Sydney, NSW 2006, AustraliaDepartment of Infectious Diseases and Immunology, Central Clinical School, The University of Sydney, Sydney, NSW 2006, AustraliaDepartment of Infectious Diseases and Immunology, Central Clinical School, The University of Sydney, Sydney, NSW 2006, AustraliaDepartment of Radiation Oncology, Royal Prince Alfred Hospital, Sydney, NSW 2050, AustraliaBackground. Human cancer is a three-dimensional (3D) structure consisting of neighboring cells, extracellular matrix, and blood vessels. It is therefore critical to mimic the cancer cells and their surrounding environment during in vitro study. Our aim was to establish a 3D cancer model using a synthetic composite scaffold. Methods. High-density low-volume seeding was used to promote attachment of a non-small-cell lung cancer cell line (NCI-H460) to scaffolds. Growth patterns in 3D culture were compared with those of monolayers. Immunohistochemistry was conducted to compare the expression of Ki67, CD44, and carbonic anhydrase IX. Results. NCI-H460 readily attached to the scaffold without surface pretreatment at a rate of 35% from a load of 1.5 × 106 cells. Most cells grew vertically to form clumps along the surface of the scaffold, and cell morphology resembled tissue origin; 2D cultures exhibited characteristics of adherent epithelial cancer cell lines. Expression patterns of Ki67, CD44, and CA IX varied markedly between 3D and monolayer cultures. Conclusions. The behavior of cancer cells in our 3D model is similar to tumor growth in vivo. This model will provide the basis for future study using 3D cancer culture.http://dx.doi.org/10.1155/2013/396056
spellingShingle Mei Zhang
Philip Boughton
Barbara Rose
C. Soon Lee
Angela M. Hong
The Use of Porous Scaffold as a Tumor Model
International Journal of Biomaterials
title The Use of Porous Scaffold as a Tumor Model
title_full The Use of Porous Scaffold as a Tumor Model
title_fullStr The Use of Porous Scaffold as a Tumor Model
title_full_unstemmed The Use of Porous Scaffold as a Tumor Model
title_short The Use of Porous Scaffold as a Tumor Model
title_sort use of porous scaffold as a tumor model
url http://dx.doi.org/10.1155/2013/396056
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