Detection of antibodies to non-structural proteins of foot-and-mouth disease virus (review)
Foot-and-mouth disease (FMD) isa highlycontagiousviraldiseaseof cloven-hoovedanimals that can causeepidemicsandgreateconomic losses. Thedisease remains a huge problem in developing countries and poses a constant threat to developed countries. Detection of antibodies to FMD virus non-structural prote...
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Da Vinci Media
2023-09-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/738 |
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| author | A. S. Yakovleva A. V. Scherbakov |
| author_facet | A. S. Yakovleva A. V. Scherbakov |
| author_sort | A. S. Yakovleva |
| collection | DOAJ |
| description | Foot-and-mouth disease (FMD) isa highlycontagiousviraldiseaseof cloven-hoovedanimals that can causeepidemicsandgreateconomic losses. Thedisease remains a huge problem in developing countries and poses a constant threat to developed countries. Detection of antibodies to FMD virus non-structural proteins in thebloodof susceptibleanimals isan important tool for thediseasecontrol. This is theonly way for reliabledifferentiation of vaccinatedanimals fromconvalescentandvirus-carrieranimals. Variousmodificationsof solid-phaseenzymeimmunosorbentassay (ELISA) havebeen developedfordetection of antibodies toFMD virus nonstructuralproteins. RecombinantFMD virus non-structuralproteins, mostly 3АВС or 3АВ, areusedasan antigen for such assay. In ashort time, recombinant FMD virusantigen-basedELISA hasevolvedfrom an in-houselaboratory methodtocommonlyavailablecommercial test systems, mostof which have high diagnostic specificityandsensitivity. Thesaid methodis widelyusedforFMD surveillance. In thecountriesandzones freefrom FMD withoutvaccination theELISA fordetection ofantibodiesagainstFMD virus non-structuralproteinsisusedasaprimarymethodforFMD serologicalmonitoring andretrospectivediagnosis. In thecountriesandzones freefromFMD with vaccination thisELISA isusedforconfirmation of thevirus infection absencein vaccinated herds. In South America, ELISA fordetection ofantibodiesagainstFMD virus non-structuralproteins wasusedfordetection of infectedanimalsduring thediseaseeradication. Currently, it isusedfor monitoringfor thevirus circulation in stillFMD infected Asian and African countries implementingprogressive FMD controlprogramme. In Russia havingzones where anti-FMD vaccination is carriedout this methodis a mandatory tool of thedisease surveillance. The review isbasedon theanalysisof 65 publications. |
| format | Article |
| id | doaj-art-5895cb99455a48d0bb0c245103123559 |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-5895cb99455a48d0bb0c2451031235592025-02-06T09:52:10ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592023-09-0112319019610.29326/2304-196X-2023-12-3-190-196621Detection of antibodies to non-structural proteins of foot-and-mouth disease virus (review)A. S. Yakovleva0A. V. Scherbakov1FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)Foot-and-mouth disease (FMD) isa highlycontagiousviraldiseaseof cloven-hoovedanimals that can causeepidemicsandgreateconomic losses. Thedisease remains a huge problem in developing countries and poses a constant threat to developed countries. Detection of antibodies to FMD virus non-structural proteins in thebloodof susceptibleanimals isan important tool for thediseasecontrol. This is theonly way for reliabledifferentiation of vaccinatedanimals fromconvalescentandvirus-carrieranimals. Variousmodificationsof solid-phaseenzymeimmunosorbentassay (ELISA) havebeen developedfordetection of antibodies toFMD virus nonstructuralproteins. RecombinantFMD virus non-structuralproteins, mostly 3АВС or 3АВ, areusedasan antigen for such assay. In ashort time, recombinant FMD virusantigen-basedELISA hasevolvedfrom an in-houselaboratory methodtocommonlyavailablecommercial test systems, mostof which have high diagnostic specificityandsensitivity. Thesaid methodis widelyusedforFMD surveillance. In thecountriesandzones freefrom FMD withoutvaccination theELISA fordetection ofantibodiesagainstFMD virus non-structuralproteinsisusedasaprimarymethodforFMD serologicalmonitoring andretrospectivediagnosis. In thecountriesandzones freefromFMD with vaccination thisELISA isusedforconfirmation of thevirus infection absencein vaccinated herds. In South America, ELISA fordetection ofantibodiesagainstFMD virus non-structuralproteins wasusedfordetection of infectedanimalsduring thediseaseeradication. Currently, it isusedfor monitoringfor thevirus circulation in stillFMD infected Asian and African countries implementingprogressive FMD controlprogramme. In Russia havingzones where anti-FMD vaccination is carriedout this methodis a mandatory tool of thedisease surveillance. The review isbasedon theanalysisof 65 publications.https://veterinary.arriah.ru/jour/article/view/738reviewfoot-and-mouth disease virusnon-structural proteinsenzyme-linked immunosorbent assay |
| spellingShingle | A. S. Yakovleva A. V. Scherbakov Detection of antibodies to non-structural proteins of foot-and-mouth disease virus (review) Ветеринария сегодня review foot-and-mouth disease virus non-structural proteins enzyme-linked immunosorbent assay |
| title | Detection of antibodies to non-structural proteins of foot-and-mouth disease virus (review) |
| title_full | Detection of antibodies to non-structural proteins of foot-and-mouth disease virus (review) |
| title_fullStr | Detection of antibodies to non-structural proteins of foot-and-mouth disease virus (review) |
| title_full_unstemmed | Detection of antibodies to non-structural proteins of foot-and-mouth disease virus (review) |
| title_short | Detection of antibodies to non-structural proteins of foot-and-mouth disease virus (review) |
| title_sort | detection of antibodies to non structural proteins of foot and mouth disease virus review |
| topic | review foot-and-mouth disease virus non-structural proteins enzyme-linked immunosorbent assay |
| url | https://veterinary.arriah.ru/jour/article/view/738 |
| work_keys_str_mv | AT asyakovleva detectionofantibodiestononstructuralproteinsoffootandmouthdiseasevirusreview AT avscherbakov detectionofantibodiestononstructuralproteinsoffootandmouthdiseasevirusreview |