Determination of 8 Endogenous Alkaloid Components in Boletus Using Ultrahigh-Performance Liquid Chromatography Combined with Quadrupole-Time of Flight Mass Spectrometry
A ultrahigh performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-TOF/MS) method was developed for simultaneous determination of 8 endogenous alkaloid compounds in Boletus. Boletus samples were extracted by 50% (V/V) methanol-water solution, then separate...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/2020/8865725 |
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| author | Junmei Ma Qiang Li Sufang Fan Liangna He Lei Sun Di Wen Hongwen Zhai Yan Zhang |
| author_facet | Junmei Ma Qiang Li Sufang Fan Liangna He Lei Sun Di Wen Hongwen Zhai Yan Zhang |
| author_sort | Junmei Ma |
| collection | DOAJ |
| description | A ultrahigh performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-TOF/MS) method was developed for simultaneous determination of 8 endogenous alkaloid compounds in Boletus. Boletus samples were extracted by 50% (V/V) methanol-water solution, then separated by CORTECS UPLC HILIC column using a binary solvent system by gradient elution. The analytes were determined by Q-TOF/MS in TOF MS and information dependent acquisition (IDA)-MS/MS mode. The results showed that mass accuracy error of the 8 endogenous alkaloids were lower than 5.0 × 10−6, good linear relationship was got in range of 0.2–500 μg/L, and correlation coefficient was higher than 0.9990. The limit of detection was in the range of 0.002–0.100 mg/kg and the limit of quantification was in the range of 0.004–0.200 mg/kg. Recovery of the method was in range of 80.1%–101.5% with spike levels of 0.004–2.00 mg/kg, relative standard deviations were lower than 10%. The method was simple, specific, and reliable. It could be used for the rapid screening and quantitative analysis of 8 endogenous alkaloids in Boletus. |
| format | Article |
| id | doaj-art-588e08fe812141219d8d43b45351eb10 |
| institution | Kabale University |
| issn | 0146-9428 1745-4557 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Food Quality |
| spelling | doaj-art-588e08fe812141219d8d43b45351eb102025-02-03T01:28:16ZengWileyJournal of Food Quality0146-94281745-45572020-01-01202010.1155/2020/88657258865725Determination of 8 Endogenous Alkaloid Components in Boletus Using Ultrahigh-Performance Liquid Chromatography Combined with Quadrupole-Time of Flight Mass SpectrometryJunmei Ma0Qiang Li1Sufang Fan2Liangna He3Lei Sun4Di Wen5Hongwen Zhai6Yan Zhang7Hebei Key Laboratory of Forensic Medicine, College of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, ChinaHebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang 050227, ChinaHebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang 050227, ChinaHebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang 050227, ChinaHebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang 050227, ChinaHebei Key Laboratory of Forensic Medicine, College of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, ChinaHebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang 050227, ChinaHebei Key Laboratory of Forensic Medicine, College of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, ChinaA ultrahigh performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-TOF/MS) method was developed for simultaneous determination of 8 endogenous alkaloid compounds in Boletus. Boletus samples were extracted by 50% (V/V) methanol-water solution, then separated by CORTECS UPLC HILIC column using a binary solvent system by gradient elution. The analytes were determined by Q-TOF/MS in TOF MS and information dependent acquisition (IDA)-MS/MS mode. The results showed that mass accuracy error of the 8 endogenous alkaloids were lower than 5.0 × 10−6, good linear relationship was got in range of 0.2–500 μg/L, and correlation coefficient was higher than 0.9990. The limit of detection was in the range of 0.002–0.100 mg/kg and the limit of quantification was in the range of 0.004–0.200 mg/kg. Recovery of the method was in range of 80.1%–101.5% with spike levels of 0.004–2.00 mg/kg, relative standard deviations were lower than 10%. The method was simple, specific, and reliable. It could be used for the rapid screening and quantitative analysis of 8 endogenous alkaloids in Boletus.http://dx.doi.org/10.1155/2020/8865725 |
| spellingShingle | Junmei Ma Qiang Li Sufang Fan Liangna He Lei Sun Di Wen Hongwen Zhai Yan Zhang Determination of 8 Endogenous Alkaloid Components in Boletus Using Ultrahigh-Performance Liquid Chromatography Combined with Quadrupole-Time of Flight Mass Spectrometry Journal of Food Quality |
| title | Determination of 8 Endogenous Alkaloid Components in Boletus Using Ultrahigh-Performance Liquid Chromatography Combined with Quadrupole-Time of Flight Mass Spectrometry |
| title_full | Determination of 8 Endogenous Alkaloid Components in Boletus Using Ultrahigh-Performance Liquid Chromatography Combined with Quadrupole-Time of Flight Mass Spectrometry |
| title_fullStr | Determination of 8 Endogenous Alkaloid Components in Boletus Using Ultrahigh-Performance Liquid Chromatography Combined with Quadrupole-Time of Flight Mass Spectrometry |
| title_full_unstemmed | Determination of 8 Endogenous Alkaloid Components in Boletus Using Ultrahigh-Performance Liquid Chromatography Combined with Quadrupole-Time of Flight Mass Spectrometry |
| title_short | Determination of 8 Endogenous Alkaloid Components in Boletus Using Ultrahigh-Performance Liquid Chromatography Combined with Quadrupole-Time of Flight Mass Spectrometry |
| title_sort | determination of 8 endogenous alkaloid components in boletus using ultrahigh performance liquid chromatography combined with quadrupole time of flight mass spectrometry |
| url | http://dx.doi.org/10.1155/2020/8865725 |
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