A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and Its Application to Italian Greater Horseshoe Bat Faecal Sample Surveys
Bats are source of coronaviruses closely related to the severe acute respiratory syndrome (SARS) virus. Numerous studies have been carried out to identify new bat viruses related to SARS-coronavirus (bat-SARS-like CoVs) using a reverse-transcribed-polymerase chain reaction assay. However, a qualitat...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/989514 |
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| author | Andrea Balboni Laura Gallina Alessandra Palladini Santino Prosperi Mara Battilani |
| author_facet | Andrea Balboni Laura Gallina Alessandra Palladini Santino Prosperi Mara Battilani |
| author_sort | Andrea Balboni |
| collection | DOAJ |
| description | Bats are source of coronaviruses closely related to the severe acute respiratory syndrome (SARS) virus. Numerous studies have been carried out to identify new bat viruses related to SARS-coronavirus (bat-SARS-like CoVs) using a reverse-transcribed-polymerase chain reaction assay. However, a qualitative PCR could underestimate the prevalence of infection, affecting the epidemiological evaluation of bats in viral ecology. In this work an SYBR Green-real time PCR assay was developed for diagnosing infection with SARS-related coronaviruses from bat guano and was applied as screening tool in a survey carried out on 45 greater horseshoe bats (Rhinolophus ferrumequinum) sampled in Italy in 2009. The assay showed high sensitivity and reproducibility. Its application on bats screening resulted in a prevalence of 42%. This method could be suitable as screening tool in epidemiological surveys about the presence of bat-SARS-like CoVs, consequently to obtain a more realistic scenario of the viral prevalence in the population. |
| format | Article |
| id | doaj-art-5857a729be14430c877c2683715f19cd |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-5857a729be14430c877c2683715f19cd2025-02-03T05:53:56ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/989514989514A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and Its Application to Italian Greater Horseshoe Bat Faecal Sample SurveysAndrea Balboni0Laura Gallina1Alessandra Palladini2Santino Prosperi3Mara Battilani4Dipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, ItalyDipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, ItalyDipartimento di Biologia Animale, Università degli Studi di Pavia, Via Taramelli 24, 27100 Pavia, ItalyDipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, ItalyDipartimento di Scienze Mediche Veterinarie, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, ItalyBats are source of coronaviruses closely related to the severe acute respiratory syndrome (SARS) virus. Numerous studies have been carried out to identify new bat viruses related to SARS-coronavirus (bat-SARS-like CoVs) using a reverse-transcribed-polymerase chain reaction assay. However, a qualitative PCR could underestimate the prevalence of infection, affecting the epidemiological evaluation of bats in viral ecology. In this work an SYBR Green-real time PCR assay was developed for diagnosing infection with SARS-related coronaviruses from bat guano and was applied as screening tool in a survey carried out on 45 greater horseshoe bats (Rhinolophus ferrumequinum) sampled in Italy in 2009. The assay showed high sensitivity and reproducibility. Its application on bats screening resulted in a prevalence of 42%. This method could be suitable as screening tool in epidemiological surveys about the presence of bat-SARS-like CoVs, consequently to obtain a more realistic scenario of the viral prevalence in the population.http://dx.doi.org/10.1100/2012/989514 |
| spellingShingle | Andrea Balboni Laura Gallina Alessandra Palladini Santino Prosperi Mara Battilani A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and Its Application to Italian Greater Horseshoe Bat Faecal Sample Surveys The Scientific World Journal |
| title | A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and Its Application to Italian Greater Horseshoe Bat Faecal Sample Surveys |
| title_full | A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and Its Application to Italian Greater Horseshoe Bat Faecal Sample Surveys |
| title_fullStr | A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and Its Application to Italian Greater Horseshoe Bat Faecal Sample Surveys |
| title_full_unstemmed | A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and Its Application to Italian Greater Horseshoe Bat Faecal Sample Surveys |
| title_short | A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and Its Application to Italian Greater Horseshoe Bat Faecal Sample Surveys |
| title_sort | real time pcr assay for bat sars like coronavirus detection and its application to italian greater horseshoe bat faecal sample surveys |
| url | http://dx.doi.org/10.1100/2012/989514 |
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