Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column
Tocopherol separations employing the same Kinetex™ C18 column via supercritical fluid chromatography (SFC) and reversed-phase liquid chromatography (RP-LC) were compared. The application of the SFC system with UV diode array detection (DAD) resulted in rapid separation of all four tocopherol homolog...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/5307340 |
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| author | Elise Urvaka Inga Mišina Arianne Soliven Paweł Górnaś |
| author_facet | Elise Urvaka Inga Mišina Arianne Soliven Paweł Górnaś |
| author_sort | Elise Urvaka |
| collection | DOAJ |
| description | Tocopherol separations employing the same Kinetex™ C18 column via supercritical fluid chromatography (SFC) and reversed-phase liquid chromatography (RP-LC) were compared. The application of the SFC system with UV diode array detection (DAD) resulted in rapid separation of all four tocopherol homologues with a total analysis time below 2 min. The RP-LC approach could not separate the isomers β and γ. The developed SFC-DAD method was precise, accurate, and most importantly more environmentally friendlier compared to the RP-LC method due to the 125-fold decrease in methanol consumption. The present study illustrated the selectivity differences between LC and SFC and how the C18 column can be used for tocopherol characterization. The optimized SFC method was successfully applied for the tocopherol determination in the seeds of nine different fruit species. |
| format | Article |
| id | doaj-art-58508869819e4cf7853d13beb349d85d |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-58508869819e4cf7853d13beb349d85d2025-02-03T06:12:47ZengWileyJournal of Chemistry2090-90632090-90712019-01-01201910.1155/2019/53073405307340Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 ColumnElise Urvaka0Inga Mišina1Arianne Soliven2Paweł Górnaś3Institute of Horticulture, Latvia University of Life Sciences and Technologies, Graudu 1, Dobele LV-3701, LatviaInstitute of Horticulture, Latvia University of Life Sciences and Technologies, Graudu 1, Dobele LV-3701, LatviaGrupo de Análisis de Contaminantes Traza, Cátedra de Farmacognosia y Productos Naturales, Facultad de Química, Universidad de la República, General Flores 2124, 11800 Montevideo, UruguayInstitute of Horticulture, Latvia University of Life Sciences and Technologies, Graudu 1, Dobele LV-3701, LatviaTocopherol separations employing the same Kinetex™ C18 column via supercritical fluid chromatography (SFC) and reversed-phase liquid chromatography (RP-LC) were compared. The application of the SFC system with UV diode array detection (DAD) resulted in rapid separation of all four tocopherol homologues with a total analysis time below 2 min. The RP-LC approach could not separate the isomers β and γ. The developed SFC-DAD method was precise, accurate, and most importantly more environmentally friendlier compared to the RP-LC method due to the 125-fold decrease in methanol consumption. The present study illustrated the selectivity differences between LC and SFC and how the C18 column can be used for tocopherol characterization. The optimized SFC method was successfully applied for the tocopherol determination in the seeds of nine different fruit species.http://dx.doi.org/10.1155/2019/5307340 |
| spellingShingle | Elise Urvaka Inga Mišina Arianne Soliven Paweł Górnaś Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column Journal of Chemistry |
| title | Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column |
| title_full | Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column |
| title_fullStr | Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column |
| title_full_unstemmed | Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column |
| title_short | Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column |
| title_sort | rapid separation of all four tocopherol homologues in selected fruit seeds via supercritical fluid chromatography using a solid core c18 column |
| url | http://dx.doi.org/10.1155/2019/5307340 |
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