Invasive mosquito species Aedes albopictus and Aedes aegypti on the Black Sea coast of the Caucasus: genetics (COI, ITS2), Wolbachia and Dirofilaria infections
The area of invasive species Aedes albopictus and Aedes aegypti is expanding. Precise identification and understanding of the genetic diversity of invasive mosquito populations allows us to develop appropriate control methods. Endosymbiotic bacterium Wolbachia pipientis has different effects on thei...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2018-08-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/1589 |
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| author | E. V. Shaikevich I. V. Patraman A. S. Bogacheva V. М. Rakova O. Р. Zelya L. A. Ganushkina |
| author_facet | E. V. Shaikevich I. V. Patraman A. S. Bogacheva V. М. Rakova O. Р. Zelya L. A. Ganushkina |
| author_sort | E. V. Shaikevich |
| collection | DOAJ |
| description | The area of invasive species Aedes albopictus and Aedes aegypti is expanding. Precise identification and understanding of the genetic diversity of invasive mosquito populations allows us to develop appropriate control methods. Endosymbiotic bacterium Wolbachia pipientis has different effects on their arthropod hosts and can influence the transmission and spread of the pathogens. The objective of the presented study was molecular-genetic identification of the Aedes mosquitoes collected in sampling sites on the Black Sea coast from 2007 to 2017; determination of genetic variability of Ae. aegypti, Ae. albopictus and their symbiotic bacteria Wolbachia; assessment of mosquitoes ability to be infected and to spread parasitic Dirofilaria. Another objective was obtaining the genetic characteristic of laboratory strain Ae. aegypti IMPITM. We investigated two markers of nuclear and mitochondrial DNA from Ae. albopictus and Ae. aegypti and compared them to DNA from Ae. cretinus and Ae. koreiсus sympatrically inhabiting the territory, as well as to one of Ae. aegypti from a laboratory line. The study of nuclear and mitochondrial DNA revealed a low level of variability in the invasive mosquitoes Ae. albopictus and Ae. aegypti collected at different collection sites and in different years. More than a half of Ae. albopictus were infected with Wolbachia, two strains of bacteria, wAlbA and wAlbB, occur in the Ae. albopictus population on the Black Sea coast. Total infection of Ae. aegypti and Ae. albopictus with dirofilariae was 1.8 %. Dirofilaria immitis was found only in mosquito abdomen, larvae of infective stage L3 were not found. D. repens larvae developed to the infective stage in the mosquitoes of both species. |
| format | Article |
| id | doaj-art-5838cd81c14f4fcdbcea23526dee24e3 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2018-08-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-5838cd81c14f4fcdbcea23526dee24e32025-02-01T09:58:06ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592018-08-0122557458510.18699/VJ18.397791Invasive mosquito species Aedes albopictus and Aedes aegypti on the Black Sea coast of the Caucasus: genetics (COI, ITS2), Wolbachia and Dirofilaria infectionsE. V. Shaikevich0I. V. Patraman1A. S. Bogacheva2V. М. Rakova3O. Р. Zelya4L. A. Ganushkina5Vavilov Institute of General Genetics, RASInstitute of Medical Parasitology and Tropical Medicine named after E. Martsinovskii, Sechenov First Moscow State Меdical UniversityMoscow State UniversityInstitute of Medical Parasitology and Tropical Medicine named after E. Martsinovskii, Sechenov First Moscow State Меdical UniversityInstitute of Medical Parasitology and Tropical Medicine named after E. Martsinovskii, Sechenov First Moscow State Меdical UniversityInstitute of Medical Parasitology and Tropical Medicine named after E. Martsinovskii, Sechenov First Moscow State Меdical UniversityThe area of invasive species Aedes albopictus and Aedes aegypti is expanding. Precise identification and understanding of the genetic diversity of invasive mosquito populations allows us to develop appropriate control methods. Endosymbiotic bacterium Wolbachia pipientis has different effects on their arthropod hosts and can influence the transmission and spread of the pathogens. The objective of the presented study was molecular-genetic identification of the Aedes mosquitoes collected in sampling sites on the Black Sea coast from 2007 to 2017; determination of genetic variability of Ae. aegypti, Ae. albopictus and their symbiotic bacteria Wolbachia; assessment of mosquitoes ability to be infected and to spread parasitic Dirofilaria. Another objective was obtaining the genetic characteristic of laboratory strain Ae. aegypti IMPITM. We investigated two markers of nuclear and mitochondrial DNA from Ae. albopictus and Ae. aegypti and compared them to DNA from Ae. cretinus and Ae. koreiсus sympatrically inhabiting the territory, as well as to one of Ae. aegypti from a laboratory line. The study of nuclear and mitochondrial DNA revealed a low level of variability in the invasive mosquitoes Ae. albopictus and Ae. aegypti collected at different collection sites and in different years. More than a half of Ae. albopictus were infected with Wolbachia, two strains of bacteria, wAlbA and wAlbB, occur in the Ae. albopictus population on the Black Sea coast. Total infection of Ae. aegypti and Ae. albopictus with dirofilariae was 1.8 %. Dirofilaria immitis was found only in mosquito abdomen, larvae of infective stage L3 were not found. D. repens larvae developed to the infective stage in the mosquitoes of both species.https://vavilov.elpub.ru/jour/article/view/1589blood-sucking mosquitoesaedes aegyptiaedes albopictusinvasionpopulationblack sea coastits2coiwolbachiadirofilaria |
| spellingShingle | E. V. Shaikevich I. V. Patraman A. S. Bogacheva V. М. Rakova O. Р. Zelya L. A. Ganushkina Invasive mosquito species Aedes albopictus and Aedes aegypti on the Black Sea coast of the Caucasus: genetics (COI, ITS2), Wolbachia and Dirofilaria infections Вавиловский журнал генетики и селекции blood-sucking mosquitoes aedes aegypti aedes albopictus invasion population black sea coast its2 coi wolbachia dirofilaria |
| title | Invasive mosquito species Aedes albopictus and Aedes aegypti on the Black Sea coast of the Caucasus: genetics (COI, ITS2), Wolbachia and Dirofilaria infections |
| title_full | Invasive mosquito species Aedes albopictus and Aedes aegypti on the Black Sea coast of the Caucasus: genetics (COI, ITS2), Wolbachia and Dirofilaria infections |
| title_fullStr | Invasive mosquito species Aedes albopictus and Aedes aegypti on the Black Sea coast of the Caucasus: genetics (COI, ITS2), Wolbachia and Dirofilaria infections |
| title_full_unstemmed | Invasive mosquito species Aedes albopictus and Aedes aegypti on the Black Sea coast of the Caucasus: genetics (COI, ITS2), Wolbachia and Dirofilaria infections |
| title_short | Invasive mosquito species Aedes albopictus and Aedes aegypti on the Black Sea coast of the Caucasus: genetics (COI, ITS2), Wolbachia and Dirofilaria infections |
| title_sort | invasive mosquito species aedes albopictus and aedes aegypti on the black sea coast of the caucasus genetics coi its2 wolbachia and dirofilaria infections |
| topic | blood-sucking mosquitoes aedes aegypti aedes albopictus invasion population black sea coast its2 coi wolbachia dirofilaria |
| url | https://vavilov.elpub.ru/jour/article/view/1589 |
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