Effect of Platelet-activating Factor on in vitro and in vivo Interleukin-6 Production
The aim of the present study was to investigate the possible effect of platelet-activating factor (PAF), by comparison with interleukin-1β and polyriboinositic/polyribocytidylic (poly I–C) acid, on IL-6 production by L 929 mouse fibroblasts. At concentrations above 1 μM PAF, the production of IL-6 b...
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| Format: | Article |
| Language: | English |
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Wiley
1994-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935194000384 |
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| author | B. Pignol T. Maisonnet P. Guinot J. M. Mencia-Huertac P. Braquet |
| author_facet | B. Pignol T. Maisonnet P. Guinot J. M. Mencia-Huertac P. Braquet |
| author_sort | B. Pignol |
| collection | DOAJ |
| description | The aim of the present study was to investigate the possible effect
of platelet-activating factor (PAF), by comparison with
interleukin-1β and polyriboinositic/polyribocytidylic (poly
I–C) acid, on IL-6 production by L 929 mouse fibroblasts. At
concentrations above 1 μM PAF, the production of IL-6 by mouse
fibroblasts was enhanced in a dose dependent fashion. At 5 μM
PAF, the peak increase (60.1 ± 19.4 U/ml) was similar to
that induced by 50 μg/ml poly I–C (60.0 ± 35.0
U/ml) and higher than the one evoked by 100 U/ml IL-1β
(3.8 ± 1.8 U/ml). The increase of 11-6 activity induced
by 5 μM PAF was maximal after a 22 h incubation period with L 929
cells. Lyso-PAF (5 μM) also increased IL-6 activity from
fibroblasts to a similar extent compared with 5 μM PAF. In
addition, the IL-6 activity induced by 5 μM PAF was still
observed when the specific PAF antagonist, BN 52021 (10 μM), was
added to the incubation medium of L 929 cells. The result suggests
that the production of IL-6 by L 929 cells evoked by PAF in
vitro is not receptor mediated. The in vivo
effect of PAF on IL-6 production was also investigated in the rat.
Two hours after intravenous injection of PAF (2 to 4 μg/kg),
a dramatic increase of IL-6 activity in rat serum was observed, this
effect being dose dependent. The increase of IL-6 induced by 3
μg/kg PAF was not observed when the animals were treated with
the PAF antagonist, BN 52021 (1 to 60 mg/kg0. These results
demonstrate that PAF modulates IL-6 production and that the in
vivo effect is receptor mediated. |
| format | Article |
| id | doaj-art-57bd6ae4733c4eaaa0491be936eb1bc6 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1994-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-57bd6ae4733c4eaaa0491be936eb1bc62025-02-03T07:25:41ZengWileyMediators of Inflammation0962-93511466-18611994-01-013428128510.1155/S0962935194000384Effect of Platelet-activating Factor on in vitro and in vivo Interleukin-6 ProductionB. Pignol0T. Maisonnet1P. Guinot2J. M. Mencia-Huertac3P. Braquet4Institut Henri Beaufour, Department of Immunology, 1 avenue des Tropiques, Les ULIS, 91952, FranceInstitut Henri Beaufour, Department of Immunology, 1 avenue des Tropiques, Les ULIS, 91952, FranceInstitut Henri Beaufour, Department of Immunology, 1 avenue des Tropiques, Les ULIS, 91952, FranceInstitut Henri Beaufour, Department of Immunology, 1 avenue des Tropiques, Les ULIS, 91952, FranceInstitut Henri Beaufour, Department of Immunology, 1 avenue des Tropiques, Les ULIS, 91952, FranceThe aim of the present study was to investigate the possible effect of platelet-activating factor (PAF), by comparison with interleukin-1β and polyriboinositic/polyribocytidylic (poly I–C) acid, on IL-6 production by L 929 mouse fibroblasts. At concentrations above 1 μM PAF, the production of IL-6 by mouse fibroblasts was enhanced in a dose dependent fashion. At 5 μM PAF, the peak increase (60.1 ± 19.4 U/ml) was similar to that induced by 50 μg/ml poly I–C (60.0 ± 35.0 U/ml) and higher than the one evoked by 100 U/ml IL-1β (3.8 ± 1.8 U/ml). The increase of 11-6 activity induced by 5 μM PAF was maximal after a 22 h incubation period with L 929 cells. Lyso-PAF (5 μM) also increased IL-6 activity from fibroblasts to a similar extent compared with 5 μM PAF. In addition, the IL-6 activity induced by 5 μM PAF was still observed when the specific PAF antagonist, BN 52021 (10 μM), was added to the incubation medium of L 929 cells. The result suggests that the production of IL-6 by L 929 cells evoked by PAF in vitro is not receptor mediated. The in vivo effect of PAF on IL-6 production was also investigated in the rat. Two hours after intravenous injection of PAF (2 to 4 μg/kg), a dramatic increase of IL-6 activity in rat serum was observed, this effect being dose dependent. The increase of IL-6 induced by 3 μg/kg PAF was not observed when the animals were treated with the PAF antagonist, BN 52021 (1 to 60 mg/kg0. These results demonstrate that PAF modulates IL-6 production and that the in vivo effect is receptor mediated.http://dx.doi.org/10.1155/S0962935194000384 |
| spellingShingle | B. Pignol T. Maisonnet P. Guinot J. M. Mencia-Huertac P. Braquet Effect of Platelet-activating Factor on in vitro and in vivo Interleukin-6 Production Mediators of Inflammation |
| title | Effect of Platelet-activating Factor on in vitro and in vivo Interleukin-6 Production |
| title_full | Effect of Platelet-activating Factor on in vitro and in vivo Interleukin-6 Production |
| title_fullStr | Effect of Platelet-activating Factor on in vitro and in vivo Interleukin-6 Production |
| title_full_unstemmed | Effect of Platelet-activating Factor on in vitro and in vivo Interleukin-6 Production |
| title_short | Effect of Platelet-activating Factor on in vitro and in vivo Interleukin-6 Production |
| title_sort | effect of platelet activating factor on in vitro and in vivo interleukin 6 production |
| url | http://dx.doi.org/10.1155/S0962935194000384 |
| work_keys_str_mv | AT bpignol effectofplateletactivatingfactoroninvitroandinvivointerleukin6production AT tmaisonnet effectofplateletactivatingfactoroninvitroandinvivointerleukin6production AT pguinot effectofplateletactivatingfactoroninvitroandinvivointerleukin6production AT jmmenciahuertac effectofplateletactivatingfactoroninvitroandinvivointerleukin6production AT pbraquet effectofplateletactivatingfactoroninvitroandinvivointerleukin6production |