Effect of Platelet-activating Factor on in vitro and in vivo Interleukin-6 Production
The aim of the present study was to investigate the possible effect of platelet-activating factor (PAF), by comparison with interleukin-1β and polyriboinositic/polyribocytidylic (poly I–C) acid, on IL-6 production by L 929 mouse fibroblasts. At concentrations above 1 μM PAF, the production of IL-6 b...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
1994-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935194000384 |
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| Summary: | The aim of the present study was to investigate the possible effect
of platelet-activating factor (PAF), by comparison with
interleukin-1β and polyriboinositic/polyribocytidylic (poly
I–C) acid, on IL-6 production by L 929 mouse fibroblasts. At
concentrations above 1 μM PAF, the production of IL-6 by mouse
fibroblasts was enhanced in a dose dependent fashion. At 5 μM
PAF, the peak increase (60.1 ± 19.4 U/ml) was similar to
that induced by 50 μg/ml poly I–C (60.0 ± 35.0
U/ml) and higher than the one evoked by 100 U/ml IL-1β
(3.8 ± 1.8 U/ml). The increase of 11-6 activity induced
by 5 μM PAF was maximal after a 22 h incubation period with L 929
cells. Lyso-PAF (5 μM) also increased IL-6 activity from
fibroblasts to a similar extent compared with 5 μM PAF. In
addition, the IL-6 activity induced by 5 μM PAF was still
observed when the specific PAF antagonist, BN 52021 (10 μM), was
added to the incubation medium of L 929 cells. The result suggests
that the production of IL-6 by L 929 cells evoked by PAF in
vitro is not receptor mediated. The in vivo
effect of PAF on IL-6 production was also investigated in the rat.
Two hours after intravenous injection of PAF (2 to 4 μg/kg),
a dramatic increase of IL-6 activity in rat serum was observed, this
effect being dose dependent. The increase of IL-6 induced by 3
μg/kg PAF was not observed when the animals were treated with
the PAF antagonist, BN 52021 (1 to 60 mg/kg0. These results
demonstrate that PAF modulates IL-6 production and that the in
vivo effect is receptor mediated. |
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| ISSN: | 0962-9351 1466-1861 |