Polymorphisms in the leptin gene and their role in adolescent obesity and metabolic health
The leptin (LEP) gene polymorphism is one of the most common polymorphisms and is believed to be associated with high circulating LEP levels that can occur in obese individuals. Thus, this study purposed to determine the relationship of LEP1 and LEP2 gene polymorphisms with overweight/obese and non-...
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Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology
2025-03-01
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| Series: | Journal of Advanced Biotechnology and Experimental Therapeutics |
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| Online Access: | https://www.bsmiab.org/jabet/?mno=204605 |
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| author | Raden Ayu Tanzila Irfannuddin Irfannuddin Yudianita Kesuma Iche Andriyani Liberty Miranti Dwi Hartanti Ni Made Elva Mayasari Wieke Anggraini Liza Chairani Thia Prameswarie Indri Ramayanti Wulan Aristi Delvi Larasari |
| author_facet | Raden Ayu Tanzila Irfannuddin Irfannuddin Yudianita Kesuma Iche Andriyani Liberty Miranti Dwi Hartanti Ni Made Elva Mayasari Wieke Anggraini Liza Chairani Thia Prameswarie Indri Ramayanti Wulan Aristi Delvi Larasari |
| author_sort | Raden Ayu Tanzila |
| collection | DOAJ |
| description | The leptin (LEP) gene polymorphism is one of the most common polymorphisms and is believed to be associated with high circulating LEP levels that can occur in obese individuals. Thus, this study purposed to determine the relationship of LEP1 and LEP2 gene polymorphisms with overweight/obese and non-obese adolescents in Palembang and their correlation with various metabolic and anthropometric parameters. This research was an analytical and observational study. A total of 50 blood samples from adolescents (aged 10-18 years) were analyzed in this study. Standard laboratory protocols for serum lipid measurements, deoxyribonucleic acid (DNA) extraction, allele analysis by polymerase chain reaction (PCR), and LEP and insulin analysis by enzyme-linked immunosorbent assay (ELISA) were employed. The study revealed the allelic frequencies for gene LEP1: non-obese (G = 0.45, A = 0.55) and obese (G = 0.87, A = 0.13), and gene LEP2: non-obese (G = 0.54, A = 0.45) and obese (G = 0.67, A = 0.33). The frequencies of alleles in the LEP1 gene showed a statistically significant association with nutritional status (p = 0.011), while there was no significant association between genotypes in the LEP gene and nutritional status. The level of LEP was 6.39 + 1.12 in non-obese and 11.57 + 1.68 in obese, with p 0.000; and the triglyceride level was 82.56 ± 43.22 in non-obese and 113.72 ± 54.03 in obese, with p 0.029. In conclusion, this study shows that LEP levels and the polymorphism in the LEP1 gene are associated with obese adolescents. [ J Adv Biotechnol Exp Ther 2025; 8(1.000): 32-43] |
| format | Article |
| id | doaj-art-579ec08280cd44568672438850510ff6 |
| institution | Kabale University |
| issn | 2616-4760 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology |
| record_format | Article |
| series | Journal of Advanced Biotechnology and Experimental Therapeutics |
| spelling | doaj-art-579ec08280cd44568672438850510ff62025-01-30T13:01:08ZengBangladesh Society for Microbiology, Immunology, and Advanced BiotechnologyJournal of Advanced Biotechnology and Experimental Therapeutics2616-47602025-03-0181324310.5455/jabet.2025.03204605Polymorphisms in the leptin gene and their role in adolescent obesity and metabolic healthRaden Ayu Tanzila0Irfannuddin Irfannuddin1Yudianita Kesuma2Iche Andriyani Liberty3Miranti Dwi Hartanti4Ni Made Elva Mayasari5Wieke Anggraini6Liza Chairani7Thia Prameswarie8Indri Ramayanti9Wulan Aristi10Delvi Larasari11Jalan KH. Balqi, Talang Banten, 13 Ulu, Seberang Ulu II, Palembang, South Sumatra, Indonesia Jalan Raya Palembang-Prabumulih, Km. 32, Indralaya, South Soumatra, Indonesia Jalan Raya Palembang-Prabumulih, Km. 32, Indralaya, South Sumatra, Indonesia Jalan Raya Palembang-Prabumulih, Km. 32, Indralaya, South Sumatra, Indonesia Jalan KH. Balqi, Talang Banten, 13 Ulu, Seberang Ulu II, Palembang, South Sumatra, Indonesia Jalan KH. Balqi, Talang Banten, 13 Ulu, Seberang Ulu II, Palembang, South Sumatra, Indonesia Jalan KH. Balqi, Talang Banten, 13 Ulu, Seberang Ulu II, Palembang, South Sumatra, Indonesia Jalan KH. Balqi, Talang Banten, 13 Ulu, Seberang Ulu II, Palembang, South Sumatra, Indonesia Jalan KH. Balqi, Talang Banten, 13 Ulu, Seberang Ulu II, Palembang, South Sumatra, Indonesia Jalan KH. Balqi, Talang Banten, 13 Ulu, Seberang Ulu II, Palembang, South Sumatra, Indonesia Jalan KH. Balqi, Talang Banten, 13 Ulu, Seberang Ulu II, Palembang, South Sumatra, Indonesia Jalan KH. Balqi, Talang Banten, 13 Ulu, Seberang Ulu II, Palembang, South Sumatra, IndonesiaThe leptin (LEP) gene polymorphism is one of the most common polymorphisms and is believed to be associated with high circulating LEP levels that can occur in obese individuals. Thus, this study purposed to determine the relationship of LEP1 and LEP2 gene polymorphisms with overweight/obese and non-obese adolescents in Palembang and their correlation with various metabolic and anthropometric parameters. This research was an analytical and observational study. A total of 50 blood samples from adolescents (aged 10-18 years) were analyzed in this study. Standard laboratory protocols for serum lipid measurements, deoxyribonucleic acid (DNA) extraction, allele analysis by polymerase chain reaction (PCR), and LEP and insulin analysis by enzyme-linked immunosorbent assay (ELISA) were employed. The study revealed the allelic frequencies for gene LEP1: non-obese (G = 0.45, A = 0.55) and obese (G = 0.87, A = 0.13), and gene LEP2: non-obese (G = 0.54, A = 0.45) and obese (G = 0.67, A = 0.33). The frequencies of alleles in the LEP1 gene showed a statistically significant association with nutritional status (p = 0.011), while there was no significant association between genotypes in the LEP gene and nutritional status. The level of LEP was 6.39 + 1.12 in non-obese and 11.57 + 1.68 in obese, with p 0.000; and the triglyceride level was 82.56 ± 43.22 in non-obese and 113.72 ± 54.03 in obese, with p 0.029. In conclusion, this study shows that LEP levels and the polymorphism in the LEP1 gene are associated with obese adolescents. [ J Adv Biotechnol Exp Ther 2025; 8(1.000): 32-43]https://www.bsmiab.org/jabet/?mno=204605leptinlep genemetabolicobesitypolymorphism |
| spellingShingle | Raden Ayu Tanzila Irfannuddin Irfannuddin Yudianita Kesuma Iche Andriyani Liberty Miranti Dwi Hartanti Ni Made Elva Mayasari Wieke Anggraini Liza Chairani Thia Prameswarie Indri Ramayanti Wulan Aristi Delvi Larasari Polymorphisms in the leptin gene and their role in adolescent obesity and metabolic health Journal of Advanced Biotechnology and Experimental Therapeutics leptin lep gene metabolic obesity polymorphism |
| title | Polymorphisms in the leptin gene and their role in adolescent obesity and metabolic health |
| title_full | Polymorphisms in the leptin gene and their role in adolescent obesity and metabolic health |
| title_fullStr | Polymorphisms in the leptin gene and their role in adolescent obesity and metabolic health |
| title_full_unstemmed | Polymorphisms in the leptin gene and their role in adolescent obesity and metabolic health |
| title_short | Polymorphisms in the leptin gene and their role in adolescent obesity and metabolic health |
| title_sort | polymorphisms in the leptin gene and their role in adolescent obesity and metabolic health |
| topic | leptin lep gene metabolic obesity polymorphism |
| url | https://www.bsmiab.org/jabet/?mno=204605 |
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