Synergistic effect of blocking cancer cell invasionrevealed by computer simulations
Invasion and metastasis are the main cause of death in cancer patients. The initial step of invasion is the degradation of extracellular matrix (ECM) by primary cancer cells in a tissue. Membranous metalloproteinase MT1-MMP and soluble metalloproteinase MMP-2 are thought to play an important role in...
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AIMS Press
2015-07-01
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| Series: | Mathematical Biosciences and Engineering |
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| Online Access: | https://www.aimspress.com/article/doi/10.3934/mbe.2015.12.1189 |
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| author | Kazuhisa Ichikawa |
| author_facet | Kazuhisa Ichikawa |
| author_sort | Kazuhisa Ichikawa |
| collection | DOAJ |
| description | Invasion and metastasis are the main cause of death in cancer patients. The initial step of invasion is the degradation of extracellular matrix (ECM) by primary cancer cells in a tissue. Membranous metalloproteinase MT1-MMP and soluble metalloproteinase MMP-2 are thought to play an important role in the degradation of ECM. In the previous report, we found that the repetitive insertion of MT1-MMP to invadopodia was crucial for the effective degradation of ECM (Hoshino, D., et al., PLoS Comp. Biol., 2012, e1002479). However, the role of MMP-2 and the effect of inhibitors for these ECM-degrading proteases were still obscure. Here we investigated these two problems by using the same model as in the previous report. First we tested the effect of MMP-2 and found that while MT1-MMP played a major role in the degradation of ECM, MMP-2 played only a marginal effect on the degradation of ECM. Based on these findings, we next tested the effect of a putative inhibitor for MT1-MMP and found that such inhibitor was ineffective in blocking ECM degradation. Then we tested combined strategy including inhibitor for MT1-MMP, reduction of its turnover and its content in vesicles. A synergistic effect of combined strategy was observed in the decrease in the efficacy of ECM degradation. Our simulation study suggests the importance of combined strategy in blocking cancer invasion and metastasis. |
| format | Article |
| id | doaj-art-575f98997c4f4c64ae0c9c87415af6b6 |
| institution | Kabale University |
| issn | 1551-0018 |
| language | English |
| publishDate | 2015-07-01 |
| publisher | AIMS Press |
| record_format | Article |
| series | Mathematical Biosciences and Engineering |
| spelling | doaj-art-575f98997c4f4c64ae0c9c87415af6b62025-01-24T02:33:57ZengAIMS PressMathematical Biosciences and Engineering1551-00182015-07-011261189120210.3934/mbe.2015.12.1189Synergistic effect of blocking cancer cell invasionrevealed by computer simulationsKazuhisa Ichikawa0Division of Mathematical Oncology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai Minato-ku, Tokyo, 108-8639Invasion and metastasis are the main cause of death in cancer patients. The initial step of invasion is the degradation of extracellular matrix (ECM) by primary cancer cells in a tissue. Membranous metalloproteinase MT1-MMP and soluble metalloproteinase MMP-2 are thought to play an important role in the degradation of ECM. In the previous report, we found that the repetitive insertion of MT1-MMP to invadopodia was crucial for the effective degradation of ECM (Hoshino, D., et al., PLoS Comp. Biol., 2012, e1002479). However, the role of MMP-2 and the effect of inhibitors for these ECM-degrading proteases were still obscure. Here we investigated these two problems by using the same model as in the previous report. First we tested the effect of MMP-2 and found that while MT1-MMP played a major role in the degradation of ECM, MMP-2 played only a marginal effect on the degradation of ECM. Based on these findings, we next tested the effect of a putative inhibitor for MT1-MMP and found that such inhibitor was ineffective in blocking ECM degradation. Then we tested combined strategy including inhibitor for MT1-MMP, reduction of its turnover and its content in vesicles. A synergistic effect of combined strategy was observed in the decrease in the efficacy of ECM degradation. Our simulation study suggests the importance of combined strategy in blocking cancer invasion and metastasis.https://www.aimspress.com/article/doi/10.3934/mbe.2015.12.1189invasionmt1-mmptherapeutic targets.mmp-2cancer |
| spellingShingle | Kazuhisa Ichikawa Synergistic effect of blocking cancer cell invasionrevealed by computer simulations Mathematical Biosciences and Engineering invasion mt1-mmp therapeutic targets. mmp-2 cancer |
| title | Synergistic effect of blocking cancer cell invasionrevealed by computer simulations |
| title_full | Synergistic effect of blocking cancer cell invasionrevealed by computer simulations |
| title_fullStr | Synergistic effect of blocking cancer cell invasionrevealed by computer simulations |
| title_full_unstemmed | Synergistic effect of blocking cancer cell invasionrevealed by computer simulations |
| title_short | Synergistic effect of blocking cancer cell invasionrevealed by computer simulations |
| title_sort | synergistic effect of blocking cancer cell invasionrevealed by computer simulations |
| topic | invasion mt1-mmp therapeutic targets. mmp-2 cancer |
| url | https://www.aimspress.com/article/doi/10.3934/mbe.2015.12.1189 |
| work_keys_str_mv | AT kazuhisaichikawa synergisticeffectofblockingcancercellinvasionrevealedbycomputersimulations |