Gene metabolite relationships revealed metabolic adaptations of rice salt tolerance
Abstract Environmental stresses, particularly salinity, pose significant challenges to global crop production, notably impacting the growth and yield of rice. Integrating gene expression and metabolomics data offers valuable insights into the molecular mechanisms driving salt tolerance in plants. Th...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-025-86604-9 |
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| author | Mojdeh Akbarzadeh Lelekami Mohammad Hadi Pahlevani Khalil Zaynali Nezhad Keyvan Mahdavi Mashaki |
| author_facet | Mojdeh Akbarzadeh Lelekami Mohammad Hadi Pahlevani Khalil Zaynali Nezhad Keyvan Mahdavi Mashaki |
| author_sort | Mojdeh Akbarzadeh Lelekami |
| collection | DOAJ |
| description | Abstract Environmental stresses, particularly salinity, pose significant challenges to global crop production, notably impacting the growth and yield of rice. Integrating gene expression and metabolomics data offers valuable insights into the molecular mechanisms driving salt tolerance in plants. This study examined the effects of high salinity on the roots and shoots of rice genotypes with contrasting tolerances: CSR28 (tolerant) and IR28 (sensitive) at the seedling stage. Our phenotypic and physiological assessments indicated significant differences in response to prolonged salinity exposure between the two genotypes. Notably, osmoprotectants, including amino acids and sugars, exhibited increased accumulation, whereas most organic acids showed a decline. Linear regression analyses established significant correlations between the levels of proline, myoinositol, catalase (CAT), and superoxide dismutase (SOD) and their respective encoding genes: OsP5CS2, OsIMP, OsNCA1a, and OsSOD-Fe. Furthermore, a relationship was identified between H2O2 content and the expression of glycolate oxidase (GLO), highlighting its role in initiating defense mechanisms under salinity stress. Our findings indicated specific metabolites and genes associated with distinct organs, genotypes, and timepoints that can serve as biomarkers for the development of new salt-tolerant rice varieties. |
| format | Article |
| id | doaj-art-5751229cdba04294969c3c02c22d12a2 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-5751229cdba04294969c3c02c22d12a22025-01-19T12:24:20ZengNature PortfolioScientific Reports2045-23222025-01-0115111410.1038/s41598-025-86604-9Gene metabolite relationships revealed metabolic adaptations of rice salt toleranceMojdeh Akbarzadeh Lelekami0Mohammad Hadi Pahlevani1Khalil Zaynali Nezhad2Keyvan Mahdavi Mashaki3Plant Breeding and Biotechnology Department, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural ResourcesPlant Breeding and Biotechnology Department, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural ResourcesPlant Breeding and Biotechnology Department, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural ResourcesRice Research Institute of Iran, Mazandaran Branch, Agricultural Research, Education and Extension Organization (AREEO)Abstract Environmental stresses, particularly salinity, pose significant challenges to global crop production, notably impacting the growth and yield of rice. Integrating gene expression and metabolomics data offers valuable insights into the molecular mechanisms driving salt tolerance in plants. This study examined the effects of high salinity on the roots and shoots of rice genotypes with contrasting tolerances: CSR28 (tolerant) and IR28 (sensitive) at the seedling stage. Our phenotypic and physiological assessments indicated significant differences in response to prolonged salinity exposure between the two genotypes. Notably, osmoprotectants, including amino acids and sugars, exhibited increased accumulation, whereas most organic acids showed a decline. Linear regression analyses established significant correlations between the levels of proline, myoinositol, catalase (CAT), and superoxide dismutase (SOD) and their respective encoding genes: OsP5CS2, OsIMP, OsNCA1a, and OsSOD-Fe. Furthermore, a relationship was identified between H2O2 content and the expression of glycolate oxidase (GLO), highlighting its role in initiating defense mechanisms under salinity stress. Our findings indicated specific metabolites and genes associated with distinct organs, genotypes, and timepoints that can serve as biomarkers for the development of new salt-tolerant rice varieties.https://doi.org/10.1038/s41598-025-86604-9OsmolyteGlycolate oxidaseBiomarkerSalinity toleranceLinear regression |
| spellingShingle | Mojdeh Akbarzadeh Lelekami Mohammad Hadi Pahlevani Khalil Zaynali Nezhad Keyvan Mahdavi Mashaki Gene metabolite relationships revealed metabolic adaptations of rice salt tolerance Scientific Reports Osmolyte Glycolate oxidase Biomarker Salinity tolerance Linear regression |
| title | Gene metabolite relationships revealed metabolic adaptations of rice salt tolerance |
| title_full | Gene metabolite relationships revealed metabolic adaptations of rice salt tolerance |
| title_fullStr | Gene metabolite relationships revealed metabolic adaptations of rice salt tolerance |
| title_full_unstemmed | Gene metabolite relationships revealed metabolic adaptations of rice salt tolerance |
| title_short | Gene metabolite relationships revealed metabolic adaptations of rice salt tolerance |
| title_sort | gene metabolite relationships revealed metabolic adaptations of rice salt tolerance |
| topic | Osmolyte Glycolate oxidase Biomarker Salinity tolerance Linear regression |
| url | https://doi.org/10.1038/s41598-025-86604-9 |
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